LOCUS       sequence-404608-       11086 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1288
FEATURES             Location/Qualifiers
     source          1..11086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6943..7531
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6996..7016)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7414..7425)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7603..7836
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7914..8035
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7951..7970)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8005..8024
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8075..8210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8230..8249)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8231..8249)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8259..8276)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8259..8275)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8268..8290)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8417..8872
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8504..8523)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8714..8735
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8898..9002
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9003..9071,9072..9863)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9221..9240)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10034..10622
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10523..10542
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10776..10793
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10910..10931
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10946..10951,10952..10969,10970..10976)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10984..11000
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10989..11011
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11008..11024
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11008..11024
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11043..11063
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11045..11063
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg cacgaatccg agaggagctc
     4141 tcgtctcggg gtgccctgcg gggagccggc agagctcgga ggtgatgcta gcgaggagga
     4201 tcacccccaa gtctgtgcca agtgctgcgc acaattcact gacccaactg aattcctcgc
     4261 ccaccagaac gcatgttcta ctgaccctcc tgtaatggtg ataattgggg gccaggagaa
     4321 ccccaacaac tcttcggcct cctctgaacc ccggcctgag ggtcacaata atcctcaggt
     4381 catggacaca gagcatagca accccccaga ttctgggtcc tccgtgccca cggatcccac
     4441 ctggggccca gagaggagag gagaggagtc ttcagggcat ttcctggtcg ctgccacagg
     4501 ggcagaaacg cccaagcagg ccttcttcca cctttaccac ccactggggt cacagcatcc
     4561 tttctctgct ggaggggttg ggcgaagcca caaacccacc cctgcccctt ccccagcctt
     4621 gccaggcagc acagatcagc tgattgcctc gcctcatctg gcattcccaa gcaccacggg
     4681 actactggca gcacagtgtc ttggggcagc ccgaggcctt gaggccactg cctccccagg
     4741 gctcctgaag ccaaagaatg gaagtggtga gctgagctac ggagaagtga tgggtccctt
     4801 ggagaagcct ggtggaaggc acaaatgccg cttctgtgcc aaagtatttg gcagtgacag
     4861 tgccctgcag atccaccttc gttcccacac gggtgagagg ccctataagt gcaatgtctg
     4921 tggaaaccgt tttaccaccc gtggcaacct caaagtgcat ttccaccggc atcgtgagaa
     4981 gtacccacat gtgcagatga acccacaccc agtaccagag cacctagact atgtcattac
     5041 cagcagtggc ttgccttatg gtatgtccgt gccaccagag aaggccgagg aggaggcagc
     5101 cactccaggt ggaggggttg agcgcaagcc tctggtggcc tccacaacag cactcagtgc
     5161 cacagagagc ctgactctgc tctccaccag tgcaggcaca gccacggctc caggactccc
     5221 tgctttcaat aagtttgtgc tcatgaaagc agtggaaccc aagaataaag ctgatgaaaa
     5281 caccccccca gggagtgagg gctcagccat cagtggagtg gcagaaagta gcacggcaac
     5341 tcgcatgcaa ctaagtaagt tggtgacttc actaccaagc tgggcactgc ttaccaacca
     5401 cttcaagtcc actggcagct tccccttccc ctatgtgcta gagcccttgg gggcctcacc
     5461 ctctgagaca tcaaagctgc agcaactggt agaaaagatt gaccggcaag gagctgtggc
     5521 ggtgacctca gctgcctcag gagcccccac cacctctgcc cctgcacctt catcctcagc
     5581 ctcttctgga cctaaccagt gtgtcatctg tctccgagtg cttagctgtc ctcgggccct
     5641 acgccttcat tatggccaac atggaggtga gaggcccttc aaatgcaaag tgtgtggcag
     5701 agccttctcc accaggggta atctgcgtgc acatttcgtg ggccacaagg ccagtccagc
     5761 tgcccgggca cagaattcct gccccatctg ccagaagaag ttcaccaatg ctgtcactct
     5821 gcagcagcat gtccggatgc acctgggggg ccagatcccc aacggtggta ctgcactccc
     5881 tgaaggtgga ggagctgctc aggagaatgg ctccgagcaa tctacagtct ccggggcacg
     5941 gagtttcccc cagcagcagt cccagcagcc atcaccggaa gaggagttgt ctgaggagga
     6001 ggaagaggag gatgaggaag aagaggaaga tgtgactgat gaagattccc tggcagggag
     6061 aggctcagag agtggaggtg agaaggcaat atcagtgaga ggtgattcag aagaggcatc
     6121 tggggcagag gaggaggtgg ggacagtggc ggcagcagcc acagctggga aggagatgga
     6181 cagtaatgag aaaactactc aacagtcttc tttgccacca ccaccaccac ctgacagcct
     6241 ggatcagcct cagccaatgg agcagggaag cagtggtgtt ttaggaggca aggaagaggg
     6301 gggcaaaccg gagagaagct caagtccggc atcagcactc accccagaag gggaagccac
     6361 cagcgtgacc ttggtagagg agctgagcct gcaggaggca atgagaaagg agccaggaga
     6421 gagcagcagc agaaaggcct gcgaagtgtg tggccaggcc tttccctccc aggcagctct
     6481 ggaggagcat cagaagaccc accccaagga ggggccgctc ttcacttgtg ttttctgcag
     6541 gcagggcttt cttgagcggg ctaccctcaa gaagcatatg ctcctggcac accaccagaa
     6601 ccagtatgtg gcattcctgt caaatggcct gcccatgaag ccctggaatt ccagctccac
     6661 ctccactacc actccaagcc tggccccacc agtgctgttt ggcctaggaa ctgtggctgg
     6721 gaaggtgcct ccaacaatgg gatccaggga agccaaggag aagacagccc ccctcctatt
     6781 tcagcctcct gcacccaagg cagtgcctga gaagcccatc atagacaaga agtagtaatg
     6841 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     6901 cgattatgct gaacaataat gtctgccacg cgttaagtcg acaatcaacc tctggattac
     6961 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     7021 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     7081 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     7141 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     7201 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     7261 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     7321 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     7381 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7441 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     7501 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     7561 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     7621 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     7681 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7741 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7801 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     7861 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     7921 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7981 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8041 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8101 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8161 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     8221 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     8281 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     8341 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     8401 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     8461 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     8521 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     8581 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     8641 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     8701 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     8761 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     8821 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     8881 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     8941 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9001 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     9061 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     9121 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     9181 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     9241 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     9301 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     9361 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     9421 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     9481 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     9541 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     9601 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     9661 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     9721 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     9781 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     9841 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     9901 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     9961 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    10021 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    10081 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    10141 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    10201 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    10261 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10321 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    10381 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    10441 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    10501 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    10561 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    10621 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    10681 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    10741 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    10801 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    10861 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    10921 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    10981 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    11041 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagc
//
