LOCUS       sequence-404821-        3253 bp    DNA     circular SYN 15-APR-2025
DEFINITION  DAT promoter and native 5'UTR (approximately 1 kb upstream of start 
            codon) from C. roseus in a MoClo compatible L0 plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Cole-Osborn LF, McCallan SA, Prifti O, Abu R, Sjoelund V, 
            Lee-Parsons CWT
  TITLE     The role of the Golden2-like (GLK) transcription factor in 
            regulating terpenoid indole alkaloid biosynthesis in Catharanthus 
            roseus.
  JOURNAL   Plant Cell Rep. 2024 May 14;43(6):141. doi: 
            10.1007/s00299-024-03208-9.
  PUBMED    38743349
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2024-05-14"; volume: "43(6):141. doi"; pages: " 
            10.1007/s00299-024-03208-9"
COMMENT     Sequence Label: pUN1215 - pL0_pDAT (pro + 5U)
FEATURES             Location/Qualifiers
     source          1..3253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     93..110
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1230..1248)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1316..1420
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1642..2433
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2604..3192
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3093..3112
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
       61 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
      121 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg
      181 gtctcaggag tgttcaaact atcaaattcc aagtgtttgt attgtcctct caaactcatc
      241 ataagcaaaa agctaagcat ccaaccataa aacattaccc tcttcaccta gaaactttca
      301 tgaacggata ttgcttacaa aatgtgtctt gaattagaac cttccaattc agaaacatta
      361 taggtgtatc ttcccactta taatattaga cataaaaata agaaatatat aaatgttatg
      421 aatttgatga acgcgtcata attgaatatt tagaataata atacaatata aggtagaata
      481 ataaagtgaa gagaaaacga agtagagaga aagtaaaact aaaaaatcaa atctggatca
      541 tattattaat atttttcgta agacatattt atagatttaa gatacaatag attacaaaac
      601 cataaaagaa atgtaatttg tcattagaag gacaaaatcc aattagaatt gaactttgag
      661 agttctagtt ccctttaaat aatatatttg aacatctatt atgtgtacat taattcaata
      721 acattccctt tttgtattta ttatgctaat ttcttgctgt gaatgttcct tttttcttta
      781 tgagtttaac aaagatttag ataattatga gagattttaa agtatttttt caatttttaa
      841 cattgaaata atttagaaat gaaatataat tattagtaat tctcacactt tgaagagaat
      901 atttttattg atttatgtaa aatttatttc caatactttc tcaactaata ttcattacat
      961 gactacacat ctactttatc tttgactttt agttattata tatatatata tatatattta
     1021 atactcttca aaagaaaaaa caactgattt atgtcatagg aactaaaacc aaaaccattc
     1081 tggccacaaa aaaaatgaaa gtatcataag aacgttgggt gggatgggca actggatagc
     1141 ctatatataa gtgttgtgtg ttggggtctg aatatataac agcaagcaaa aatgtgagac
     1201 cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     1261 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     1321 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1381 accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc
     1441 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     1501 tgactgtttt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     1561 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     1621 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     1681 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     1741 tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg
     1801 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     1861 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     1921 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     1981 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     2041 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     2101 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     2161 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     2221 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     2281 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     2341 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgta
     2401 aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag accaagttta ctcatatata
     2461 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2521 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2581 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2641 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2701 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2761 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2821 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2881 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2941 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3001 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3061 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3121 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3181 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3241 tttgctcaca tgt
//
