LOCUS       sequence-404855-        2714 bp    DNA     circular SYN 15-APR-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT1G18700
FEATURES             Location/Qualifiers
     source          1..2714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      273..861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     762..781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1015..1032
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1613..1631)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1699..1803
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2025..2714,1..102)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
       61 cattacgtaa aaggcgagat caccaaggta gtcggcaaat aactgtcaga ccaagtttac
      121 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
      181 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
      241 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
      301 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      361 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
      421 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      481 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      541 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      601 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      661 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      721 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      781 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
      841 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
      901 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      961 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     1021 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     1081 ccgattcatt aatcactctg tggtctcagg agttttattt tgtttatttc acaatatgtg
     1141 ttccattcag gtattaaata aacttttgca caaaacaaaa tggtagtaat aatgtattta
     1201 ccatggaaat agatagttga tcatttgtat tttatttaaa aattataaag gttttggcaa
     1261 ttttatctgt aaattcgtaa tactatctaa tattgtttgg tagtcgtcac atattaatct
     1321 ttttttttgt ttttgttaaa aataacatat taatcttaaa ccataattat atttaatttt
     1381 agaaaaagag agatttagta tgctgtgatc tgataagata cgattttaac cataataaaa
     1441 gagtataatt acttacttaa tgctaagagt cgatcccttt ttttatttat taagaaaaac
     1501 gcggaaaaat aaccgaagga gatttacact tatcacatcg agactctttg atcggagttt
     1561 cccaaaatcc ccgaatgtga gaccacgaag tggctcttca gtggacgaaa gggcctcgtg
     1621 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     1681 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     1741 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1801 agtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     1861 gtggcggttt tcatggcttg ttatgactgt ttttttttgg ggtacagtct atgcctcggg
     1921 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     1981 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga
     2041 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     2101 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     2161 gcgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     2221 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     2281 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     2341 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     2401 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     2461 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     2521 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     2581 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     2641 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     2701 aagctagaca ggct
//
