LOCUS       sequence-404868-       11372 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Level 2 MoClo plasmid for A. baumannii with additional Tellurite 
            resistance cassette for multi-drug resistance strains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11372)
  AUTHORS   Brychcy M, Kokodynski A, Lloyd D, Godoy VG
  TITLE     AspFlex: Molecular Tools to Study Gene Expression and Regulation in 
            Acinetobacter baumannii.
  JOURNAL   ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167.
  PUBMED    37587063
REFERENCE   2  (bases 1 to 11372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167."
COMMENT     Sequence Label: pMB2-C-TelR
FEATURES             Location/Qualifiers
     source          1..11372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..64
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    240..261
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(276..281,282..299,300..306)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    314..330
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             348..359
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             366..1043
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      1088..1159
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1175..1202
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      5163..5220
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(5244..5261)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5415..6003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5495..5514)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(6186..6280)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             6384..7178
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      7196..7282
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(7299..7958)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7873..7892
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     8120..8138
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(8142..8185)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     protein_bind    8232..8279
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      8343..8374
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     RBS             11275..11297
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ggaatcaggg gatcttgaag ttcctattcc gaagttccta ttctctagaa agtataggaa
       61 cttcagagcg cttttgaagc taattcgagc tcggtacccg gggcggccgc ttctagaggt
      121 ctcaggacat ctagagacgc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      181 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
      241 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      301 atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat
      361 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
      421 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
      481 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
      541 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
      601 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
      661 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
      721 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
      781 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
      841 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
      901 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
      961 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
     1021 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc
     1081 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1141 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1201 cgtttatacg tctcattagt cgaagagacc ctgcagttgc aagacaatat cgaccgcttg
     1261 cagcttgata aagtgatcaa agagggacga gcagctacag cactgctaag ggagatcgga
     1321 gagccactgc cgccaccagt gaaagtgaaa acaggttggt ttagtgctga ttattacggc
     1381 tttgaggaca tgctcacacg ttgggataat gatcaatcta gcctttccat ttatcacaga
     1441 gagataaaag aggagcaaca ggaacagcaa cgaaaacaac agcagataga aagagaaact
     1501 aaacaaaaac aggaagatga caaaaagaaa cggcaggact atgaaagata tttatcaaaa
     1561 tacggctatg tcaaagatgc tgagtatgac catatcagcg accatgttgc ttcagacatt
     1621 tccatgttta cacgtcttca ggcgcaggca tgggcaagta acgatatgca agagtacata
     1681 aggtgttcaa agcaaaaata cgagcagatc agcgacagaa tagcctatga gcgtgacttg
     1741 aaaaaaatcg ggcaattgag ccgtatcctg gataaagaca gacaggcact tgctgatatt
     1801 ttaccgcagt tgatgcctta ctatgagcag atgcaacagg ctgttaataa acggaaaatt
     1861 ttacttgaga atgagcgaca gccatcattt aaaccacgtt atgaccaaga acagcctaag
     1921 ccgaaaaaag acaatgacct aactttttaa tgtgttacaa tagcattcaa atgtaacaca
     1981 ttggagtgaa aaacatgaca gaagcgactt ttacctttcg ggtagaccat gacctaaagc
     2041 aagaattttc aagccttgct aagactgttg atcggtcggg ggcgcagcta atccgtgact
     2101 tcatgcgtga ctttgttaag aagcaacagg aagccgcaga ctatgacaaa tggttcaagc
     2161 agcaggtaca gatcggctta aatgaagcca atgcaggcaa gttgattcca catgaggacg
     2221 taaaggcaga gtttgccgct agacgtgctg caacattggc gaaactggca gcgaagaatg
     2281 aagattgagt ggactgaaac agctcgccaa gatcgtagaa atatctatga ttatcttgaa
     2341 gaacgcaacc ctatagcagc tattgaaatt gatgatttaa ttgaagaaaa gacagattta
     2401 cttgttgata atcgactgat ggggcgcaca ggcagacaga aagatactag ggagttagtg
     2461 atacatccgc attatgtggt tgtatatgac atcactgata taatacggat actcagagtg
     2521 ctacacacat cgcaggagtg gtcatgactt actcatgtac tttggattat ttagtgttat
     2581 aaaatcctga tttataaatt ttttttgtta aaaaagataa aagccccttg caattgcttg
     2641 gggctttacc gtaatttatg gggtacagat cttcgatact gacatatcgg caatcgaaag
     2701 cattaaggtt tgacgaccgc taatgatttc accacagggg cttaatgtac ctgtcttaaa
     2761 ttctaaggtt ttaactcgct ttgtcaagca tagaccccaa aaatttagcc aatgtctgta
     2821 actcaatctg tccatgtgtg ggtgatgagg tacagtgacg ctagcacaca tcggaaaaac
     2881 gctattacta ggggaactga acagagtagc ggacgcaatg agtagtcatt taattggcgg
     2941 ttatgagcgt gttcaggcgg tgctatcaat cgtaatcata acagtggcag cttgatacag
     3001 tgatgtcatc cctgatgcga aagcgaccga ccgacggtac atcgaatggg aatactttag
     3061 ggtgattttt aagaatcgct ctagggtgag tatttcccat tcagctctgc tccctccctc
     3121 tggtacttta atcaaaagca ctactaaaca tatgttttta aataaaaaat attgatatag
     3181 agataatatt agtaagaata attaaacaat tgaatataga taaatcattg ttaaataaag
     3241 attaattatt aaaatgaatg tatacttata tataaatcaa tgatttaaaa tatttgataa
     3301 agaaaacttt tcaaaaaaaa tataattgag attgtgtcat ttcggtcaat tcttaatatg
     3361 ttccacgcaa gttttagcta tggtgctaaa cagaaatttg ctgaaaaaga acttttcact
     3421 gaactggtta aaatgtaagc agcctgagag ccgccaaaaa ttttaaaaac aaaccgcctt
     3481 aatcatcttc aaaaaatacc tctaaaacct caccatttgc gttttaagac ccatatttca
     3541 tcctgccctt atgttcccat gctgatagct ataaagtgtc tgtaatcgct tcctatgacg
     3601 ttctaggctg ttgataactt ttggaacaac gcaaaatgtt aaaatccgat cattttttaa
     3661 cctagttatt ttcgttacag ggtaactttg gtagtattat ttcaatattt agttgttaca
     3721 ggataacaaa atatatgtta cagggtaatt aatttagcgt tacaggataa ctatagttac
     3781 agggtaacgt taaatagtta tcctgtaatt taaaaatagt tgcaggatag cataaatttt
     3841 tacaaataaa ttttataaaa agttatcctg taactaaaaa caatgttatc ctgtaactat
     3901 gatatactat atgggagtta aagacatgaa agacccgaac gatcagaaaa cacaggacat
     3961 gctaaaagag ccgccaaaaa ccaatgccga acgacaaaaa gcatatcgtg aaaaacgcaa
     4021 aagccttgat agtcaacgtt tggaggtgtt tatagataag ggtgtgtcgg atatgcttgc
     4081 ggacatggtg ggcgcagcag gggagagcca aaaagccatt ttgaccgcat tgattgagaa
     4141 agagtataag cggctgtatg cagtgaaaaa tagcaagcta aaaaagtaca ttgattcaaa
     4201 aagtaatgca gataaaaaag aaaccctcga tttatgaggg ttttttataa ctactaatgc
     4261 ctatgaaaat aagtgcttat cattttctct aatgtaccca ataaaatcgg ggttttgttc
     4321 cacaagtttc ttgatgtcat tatcatgttt cttcagtaag taagccccga tcaggatctt
     4381 gcgttttgca tcgtctgacc ttgcctgttt ttgctctcga cctttcacca cagcgtcagc
     4441 tagatccttt cttgcctttt cctgacgttc caatttttcc ttttcttcat cgagcatttg
     4501 acccaattcc atagctgttt ttttgaccat ttttaatgcc tgtgtctcaa cctattttcc
     4561 gaagtatgaa ccatcatcaa tgtgaaaagt gttctacttc aaaaactcga cttacatcaa
     4621 gatccgagcg cagcgagcaa aatcaaaaaa caaagtcaaa gccttattgc tcttgctttt
     4681 gcttttagcc tcgcagagtt cccgaagggc gcacttacgc aaaatttttg ctacgccaaa
     4741 ttttgcaagt acggtcaggg aaaccccgac accccaaccg cccaaaactt ggggcggctg
     4801 ttaataaaca ggtgatgaaa atggcaattt accattgtga aatgcagaac atttcgaggt
     4861 cagatggtcg ctcaatcgtg gcatgtgcag cataccgagc aggcgaaaaa ttgtactgtg
     4921 atacgtacgg aaaagagcag gactacacca aaaaaacagg cattgaatac acccaaattt
     4981 ttgccccaac tggggcaagt cctgacatgt tagatcgtca aaccctatgg aatcgggtag
     5041 agcaatccga actaaaaaag aacggtgaca tcaaacagga ggcaagatta gcaaaggaag
     5101 tagagatagc attgccgcat gaactggata agacacagcg tcaggcaact tgttaactgc
     5161 agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga
     5221 ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5281 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5341 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5401 gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5461 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5521 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5581 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5641 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5701 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5761 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5821 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     5881 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5941 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6001 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6061 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6121 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg
     6181 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
     6241 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatattctc
     6301 gagaagctgg ggatccgttt gatttttaat ggataatgtg atataatctt taaatactgt
     6361 agaaaagagg aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac
     6421 tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata
     6481 agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga
     6541 ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg
     6601 ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg
     6661 ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg
     6721 agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata
     6781 cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac gatctggccg
     6841 atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg
     6901 attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag
     6961 acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg
     7021 gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg
     7081 gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga
     7141 aaataaaata ttatatttta ctggatgaat tgttttagga cgtcgccggc ggcatcaaat
     7201 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     7261 cgctctcctg agtaggacaa atcctcgaga atatcaaatt acgccccgcc ctgccactca
     7321 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     7381 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     7441 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     7501 aaactcaccc agggattggc tgacacgaaa aacatattct caataaaccc tttagggaaa
     7561 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     7621 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     7681 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     7741 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     7801 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     7861 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     7921 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     7981 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     8041 gaacctctta cgtgcccgat caactcgagt gccacctgac gtctaagaaa ccattattat
     8101 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc
     8161 cttagctttc gctaaggatg atttctggaa ttcgcggccg ccccgggtac cgagctcgaa
     8221 ttggggatct tgaagtacct attccgaagt tcctattctc tagaaagtat aggaacttca
     8281 gagcgctttt gaagctgatg tgcttaaaaa cttactcaat ggaattagct cctcgagcag
     8341 aaagtcaaaa gcctccgacc ggaggctttt gacttgaggg ggatcgatcc cttatggctc
     8401 tgcacccggc tccatcacca acaggtcgcg cacgcgcttc actcggttgc ggatcgacac
     8461 tgccagccca acaaagccgg ttgccgccgc cgccaggatc gcgccgatga tgccggccac
     8521 accggccatc gcccaccagg tcgccgcctt ccggttccat tcctgctggt actgcttcgc
     8581 aatgctggac ctcggctcac cataggctga ccgctcgatg gcgtatgccg cttctcccct
     8641 tggcgtaaaa cccagcgccg caggcggcat tgccatgctg cccgccgctt tcccgaccac
     8701 gacgcgcgca ccaggcttgc ggtccagacc ttcggccacg gcgagctgcg caaggacata
     8761 atcagccgcc gacttggctc cacgcgcctc gatcagctct tgcactcgcg cgaaatcctt
     8821 ggcctccacg gccgccatga atcgcgcacg cggcgaaggc tccgcagggc cggcgtcgtg
     8881 atcgccgccg agaatgccct tcaccaagtt cgacgacacg aaaatcatgc tgacggctat
     8941 caccatcatg cagacggatc gcacgaaccc gcagaactca cccccgaaca cgagcacggc
     9001 acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc
     9061 cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca cccaggccgc cgccctcact
     9121 gcccggcacc tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa tgcttccggg
     9181 cgtcgcgctc gggctgatcg cccatcccgt tactgccccg atcccggcaa tggcaaggac
     9241 tgccagcgcc gcgatgagga agcgggtgcc ccgcttcttc atcttcgcgc ctcgggcctc
     9301 caggccgcct acctgggcga aaacatcggt gtttgtggca ttcatacgga ctcctgttgg
     9361 gccagctcgc gcacgggctg gcgggtcagc ttggcttgaa gatcgccacg cattgcggcg
     9421 atctgcttct cggcatcctt gcgcttctgc acgccttcct gctggatgcg aataacgtcc
     9481 tcgacggtct tgatgagcgt cgtctgaacc tgcttgagcg tgtccacgtc gatcaccagg
     9541 cgttggttct ccttcgccgt ttcgacggac gtgcgatgca gcagggccgc attgcgcttc
     9601 atcaggtcgt tggtggtgtc gtcgatggcc gtggccagtt cgacggcgtt cttctgctcg
     9661 ttgaggctca aggccagcat gaattgccgc ttccacgccg gcacggtgat ttcgcggatg
     9721 gtgtggaatt tatcgaccag catctggttg ttggcctgga tcatgcggat ggtcggcagg
     9781 ctctgcatgg ccgaatgttg caaggcgatc aggtcgccga tgcgcttgtc caggttggca
     9841 accatcgcat cgaggtcggc cagctcctgc acgcggccag ggtcgttccc gacattgccg
     9901 cgcagaccct cggcctgctc gcgcagctcg gcaaggcgga ccttgccggc cgcgatgtgg
     9961 acgccaagaa ggcggtgttc ctcgcgcacg gctgcgaaca tttcgtcgag cgaggcattg
    10021 cgctgcgcga tgccttgctg ggtggtctgc acttcgctga ccaggtgttc gatctgctcg
    10081 cgggtcgtgt cgaagcgcgc catgaagccc gtcgaacgga cgcgggagcg gtcgatcagc
    10141 gggccaatca ggggcaggcg ggaacggttg tcggacaaag ggccgacgtt cagggaacgg
    10201 gccttggcga caacctgggt cagtttctcg cctgcttcgt ccaggtcgct gttgcgcacc
    10261 tggtccagca ggctatcggc gtagcgggac gtgtgctcgg ccacgtcgcg gccgaactcg
    10321 gcaacggtct gcggactgcc gacctcgatc cgctgcgcga ccgcatggac ttccggcacg
    10381 tcgctttcct gcaagcccag ctcgcgcagg gttgccgggg tcatgtcaaa ggcgacgata
    10441 ggggccttgg cgtcgtgcgt cgttttcagt gcgttcatag ggttctcccg ccgtgttatt
    10501 ggttgatgcc ttccaggctc tgcgaaaggc tccgcatgag cgcctggtga gctttggccg
    10561 cctcggcgac cattgccgga ttcatgttct tggtggtgat gagcgcgagg gtgtgctgac
    10621 gccagacggg caccaggacg gatgccgttt cagagaagcg gtccagcatg tccacggcct
    10681 gcgcccgcgt gagcttcatc tgagtgacgc tcatttcatg ggacgccatg agggttgcca
    10741 ggttggcgag cttgcgcgcg aagcgttcgc gcggcttgtc gaactcgatc acgccggcct
    10801 tggccgcgcc ggcctcgggg ttctcgtcca ggaactcgcg cccggcttga atgtaggctc
    10861 tgagccggtc tacctcggcc tcatgcgtat tgagcatgtc atccaaggcg cgcaacgtgt
    10921 cccgcacgcg ctgcgctacg ccctcggctt cgtccagcaa ctggtcgagc gtcttgcggg
    10981 cgacctgata cctcacctgg cgttcaacct cacggccaag catcttctcg aaccaggtag
    11041 gcttttccgc gatcttgcgg gggtccgcgt cgaccagctt cgccacgatc tggctgattt
    11101 tgtcggccag cgcggcaact gcgccgtgct ccatcagatt cgacagctcg ttgagggaat
    11161 ccgccccgtc gatgccggcc ccgtactcgc caatcgtcgc cggcgacgcg aagagggcgg
    11221 gcaaaacctc ccccttcaat cgcgccatgt tcacgcttgt tcttccatgg tatatctcct
    11281 tcttaaagtt aaacaaaatt attcggaacc cagcatgata ttccggaaat accaactaag
    11341 tcaacggctg atatccattg ctgttgacaa ag
//
