LOCUS       sequence-404885-       11378 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Level 2 MoClo plasmid for A. baumannii with additional Tellurite 
            resistance cassette for multi-drug resistance strains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11378)
  AUTHORS   Brychcy M, Kokodynski A, Lloyd D, Godoy VG
  TITLE     AspFlex: Molecular Tools to Study Gene Expression and Regulation in 
            Acinetobacter baumannii.
  JOURNAL   ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167.
  PUBMED    37587063
REFERENCE   2  (bases 1 to 11378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167."
COMMENT     Sequence Label: pMB2a-TelR
FEATURES             Location/Qualifiers
     source          1..11378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    123..144
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(159..164,165..182,183..189)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    197..213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             231..242
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             249..926
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      971..1042
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1058..1085
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      5046..5103
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(5127..5144)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5298..5886)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5378..5397)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(6069..6163)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             6267..7061
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      7079..7165
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(7182..7841)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7756..7775
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     8003..8021
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(8025..8068)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     RBS             8268..8290
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      complement(11191..11222)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     protein_bind    complement(11286..11333)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 ggtctcacta tatctagaga cgcaatacgc aaaccgcctc tccccgcgcg ttggccgatt
       61 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      121 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      181 cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagag aaagaggaga
      241 aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta
      301 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt
      361 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt
      421 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg
      481 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga
      541 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt
      601 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga
      661 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga
      721 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta
      781 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca
      841 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg
      901 aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag
      961 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1021 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1081 ctgcgtttat acgtctcacc gggtacagag accctgcagt taacaagttg cctgacgctg
     1141 tgtcttatcc agttcatgcg gcaatgctat ctctacttcc tttgctaatc ttgcctcctg
     1201 tttgatgtca ccgttctttt ttagttcgga ttgctctacc cgattccata gggtttgacg
     1261 atctaacatg tcaggacttg ccccagttgg ggcaaaaatt tgggtgtatt caatgcctgt
     1321 ttttttggtg tagtcctgct cttttccgta cgtatcacag tacaattttt cgcctgctcg
     1381 gtatgctgca catgccacga ttgagcgacc atctgacctc gaaatgttct gcatttcaca
     1441 atggtaaatt gccattttca tcacctgttt attaacagcc gccccaagtt ttgggcggtt
     1501 ggggtgtcgg ggtttccctg accgtacttg caaaatttgg cgtagcaaaa attttgcgta
     1561 agtgcgccct tcgggaactc tgcgaggcta aaagcaaaag caagagcaat aaggctttga
     1621 ctttgttttt tgattttgct cgctgcgctc ggatcttgat gtaagtcgag tttttgaagt
     1681 agaacacttt tcacattgat gatggttcat acttcggaaa ataggttgag acacaggcat
     1741 taaaaatggt caaaaaaaca gctatggaat tgggtcaaat gctcgatgaa gaaaaggaaa
     1801 aattggaacg tcaggaaaag gcaagaaagg atctagctga cgctgtggtg aaaggtcgag
     1861 agcaaaaaca ggcaaggtca gacgatgcaa aacgcaagat cctgatcggg gcttacttac
     1921 tgaagaaaca tgataatgac atcaagaaac ttgtggaaca aaaccccgat tttattgggt
     1981 acattagaga aaatgataag cacttatttt cataggcatt agtagttata aaaaaccctc
     2041 ataaatcgag ggtttctttt ttatctgcat tactttttga atcaatgtac ttttttagct
     2101 tgctattttt cactgcatac agccgcttat actctttctc aatcaatgcg gtcaaaatgg
     2161 ctttttggct ctcccctgct gcgcccacca tgtccgcaag catatccgac acacccttat
     2221 ctataaacac ctccaaacgt tgactatcaa ggcttttgcg tttttcacga tatgcttttt
     2281 gtcgttcggc attggttttt ggcggctctt ttagcatgtc ctgtgttttc tgatcgttcg
     2341 ggtctttcat gtctttaact cccatatagt atatcatagt tacaggataa cattgttttt
     2401 agttacagga taacttttta taaaatttat ttgtaaaaat ttatgctatc ctgcaactat
     2461 ttttaaatta caggataact atttaacgtt accctgtaac tatagttatc ctgtaacgct
     2521 aaattaatta ccctgtaaca tatattttgt tatcctgtaa caactaaata ttgaaataat
     2581 actaccaaag ttaccctgta acgaaaataa ctaggttaaa aaatgatcgg attttaacat
     2641 tttgcgttgt tccaaaagtt atcaacagcc tagaacgtca taggaagcga ttacagacac
     2701 tttatagcta tcagcatggg aacataaggg caggatgaaa tatgggtctt aaaacgcaaa
     2761 tggtgaggtt ttagaggtat tttttgaaga tgattaaggc ggtttgtttt taaaattttt
     2821 ggcggctctc aggctgctta cattttaacc agttcagtga aaagttcttt ttcagcaaat
     2881 ttctgtttag caccatagct aaaacttgcg tggaacatat taagaattga ccgaaatgac
     2941 acaatctcaa ttatattttt tttgaaaagt tttctttatc aaatatttta aatcattgat
     3001 ttatatataa gtatacattc attttaataa ttaatcttta tttaacaatg atttatctat
     3061 attcaattgt ttaattattc ttactaatat tatctctata tcaatatttt ttatttaaaa
     3121 acatatgttt agtagtgctt ttgattaaag taccagaggg agggagcaga gctgaatggg
     3181 aaatactcac cctagagcga ttcttaaaaa tcaccctaaa gtattcccat tcgatgtacc
     3241 gtcggtcggt cgctttcgca tcagggatga catcactgta tcaagctgcc actgttatga
     3301 ttacgattga tagcaccgcc tgaacacgct cataaccgcc aattaaatga ctactcattg
     3361 cgtccgctac tctgttcagt tcccctagta atagcgtttt tccgatgtgt gctagcgtca
     3421 ctgtacctca tcacccacac atggacagat tgagttacag acattggcta aatttttggg
     3481 gtctatgctt gacaaagcga gttaaaacct tagaatttaa gacaggtaca ttaagcccct
     3541 gtggtgaaat cattagcggt cgtcaaacct taatgctttc gattgccgat atgtcagtat
     3601 cgaagatctg taccccataa attacggtaa agccccaagc aattgcaagg ggcttttatc
     3661 ttttttaaca aaaaaaattt ataaatcagg attttataac actaaataat ccaaagtaca
     3721 tgagtaagtc atgaccactc ctgcgatgtg tgtagcactc tgagtatccg tattatatca
     3781 gtgatgtcat atacaaccac ataatgcgga tgtatcacta actccctagt atctttctgt
     3841 ctgcctgtgc gccccatcag tcgattatca acaagtaaat ctgtcttttc ttcaattaaa
     3901 tcatcaattt caatagctgc tatagggttg cgttcttcaa gataatcata gatatttcta
     3961 cgatcttggc gagctgtttc agtccactca atcttcattc ttcgctgcca gtttcgccaa
     4021 tgttgcagca cgtctagcgg caaactctgc ctttacgtcc tcatgtggaa tcaacttgcc
     4081 tgcattggct tcatttaagc cgatctgtac ctgctgcttg aaccatttgt catagtctgc
     4141 ggcttcctgt tgcttcttaa caaagtcacg catgaagtca cggattagct gcgcccccga
     4201 ccgatcaaca gtcttagcaa ggcttgaaaa ttcttgcttt aggtcatggt ctacccgaaa
     4261 ggtaaaagtc gcttctgtca tgtttttcac tccaatgtgt tacatttgaa tgctattgta
     4321 acacattaaa aagttaggtc attgtctttt ttcggcttag gctgttcttg gtcataacgt
     4381 ggtttaaatg atggctgtcg ctcattctca agtaaaattt tccgtttatt aacagcctgt
     4441 tgcatctgct catagtaagg catcaactgc ggtaaaatat cagcaagtgc ctgtctgtct
     4501 ttatccagga tacggctcaa ttgcccgatt tttttcaagt cacgctcata ggctattctg
     4561 tcgctgatct gctcgtattt ttgctttgaa caccttatgt actcttgcat atcgttactt
     4621 gcccatgcct gcgcctgaag acgtgtaaac atggaaatgt ctgaagcaac atggtcgctg
     4681 atatggtcat actcagcatc tttgacatag ccgtattttg ataaatatct ttcatagtcc
     4741 tgccgtttct ttttgtcatc ttcctgtttt tgtttagttt ctctttctat ctgctgttgt
     4801 tttcgttgct gttcctgttg ctcctctttt atctctctgt gataaatgga aaggctagat
     4861 tgatcattat cccaacgtgt gagcatgtcc tcaaagccgt aataatcagc actaaaccaa
     4921 cctgttttca ctttcactgg tggcggcagt ggctctccga tctcccttag cagtgctgta
     4981 gctgctcgtc cctctttgat cactttatca agctgcaagc ggtcgatatt gtcttgcaac
     5041 tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa
     5101 agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5161 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5221 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5281 gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga
     5341 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5401 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5461 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5521 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5581 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5641 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5701 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     5761 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5821 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5881 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5941 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6001 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg
     6061 aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
     6121 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatt
     6181 ctcgagaagc tggggatccg tttgattttt aatggataat gtgatataat ctttaaatac
     6241 tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg gaattgaaaa
     6301 aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat
     6361 ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagt cggtataaag
     6421 ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc
     6481 ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg
     6541 aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta
     6601 tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct
     6661 atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg
     6721 ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg cgcgagctgt
     6781 atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg
     6841 gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa
     6901 gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc agggaggata
     6961 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
     7021 agaaaataaa atattatatt ttactggatg aattgtttta ggacgtcgcc ggcggcatca
     7081 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     7141 gaacgctctc ctgagtagga caaatcctcg agaatatcaa attacgcccc gccctgccac
     7201 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
     7261 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     7321 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     7381 gtgaaactca cccagggatt ggctgacacg aaaaacatat tctcaataaa ccctttaggg
     7441 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     7501 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     7561 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     7621 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     7681 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     7741 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     7801 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     7861 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     7921 ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat
     7981 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
     8041 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgccccggg taccgagctc
     8101 gaattgggga tcttgaagta cctattccga agttcctatt ctctagarag wataggaact
     8161 tcggaatagg aacttcaaga tcccctgatt ccctttgtca acagcaatgg atatcagccg
     8221 ttgacttagt tggtatttcc ggaatatcat gctgggttcc gaataatttt gtttaacttt
     8281 aagaaggaga tataccatgg aagaacaagc gtgaacatgg cgcgattgaa gggggaggtt
     8341 ttgcccgccc tcttcgcgtc gccggcgacg attggcgagt acggggccgg catcgacggg
     8401 gcggattccc tcaacgagct gtcgaatctg atggagcacg gcgcagttgc cgcgctggcc
     8461 gacaaaatca gccagatcgt ggcgaagctg gtcgacgcgg acccccgcaa gatcgcggaa
     8521 aagcctacct ggttcgagaa gatgcttggc cgtgaggttg aacgccaggt gaggtatcag
     8581 gtcgcccgca agacgctcga ccagttgctg gacgaagccg agggcgtagc gcagcgcgtg
     8641 cgggacacgt tgcgcgcctt ggatgacatg ctcaatacgc atgaggccga ggtagaccgg
     8701 ctcagagcct acattcaagc cgggcgcgag ttcctggacg agaaccccga ggccggcgcg
     8761 gccaaggccg gcgtgatcga gttcgacaag ccgcgcgaac gcttcgcgcg caagctcgcc
     8821 aacctggcaa ccctcatggc gtcccatgaa atgagcgtca ctcagatgaa gctcacgcgg
     8881 gcgcaggccg tggacatgct ggaccgcttc tctgaaacgg catccgtcct ggtgcccgtc
     8941 tggcgtcagc acaccctcgc gctcatcacc accaagaaca tgaatccggc aatggtcgcc
     9001 gaggcggcca aagctcacca ggcgctcatg cggagccttt cgcagagcct ggaaggcatc
     9061 aaccaataac acggcgggag aaccctatga acgcactgaa aacgacgcac gacgccaagg
     9121 cccctatcgt cgcctttgac atgaccccgg caaccctgcg cgagctgggc ttgcaggaaa
     9181 gcgacgtgcc ggaagtccat gcggtcgcgc agcggatcga ggtcggcagt ccgcagaccg
     9241 ttgccgagtt cggccgcgac gtggccgagc acacgtcccg ctacgccgat agcctgctgg
     9301 accaggtgcg caacagcgac ctggacgaag caggcgagaa actgacccag gttgtcgcca
     9361 aggcccgttc cctgaacgtc ggccctttgt ccgacaaccg ttcccgcctg cccctgattg
     9421 gcccgctgat cgaccgctcc cgcgtccgtt cgacgggctt catggcgcgc ttcgacacga
     9481 cccgcgagca gatcgaacac ctggtcagcg aagtgcagac cacccagcaa ggcatcgcgc
     9541 agcgcaatgc ctcgctcgac gaaatgttcg cagccgtgcg cgaggaacac cgccttcttg
     9601 gcgtccacat cgcggccggc aaggtccgcc ttgccgagct gcgcgagcag gccgagggtc
     9661 tgcgcggcaa tgtcgggaac gaccctggcc gcgtgcagga gctggccgac ctcgatgcga
     9721 tggttgccaa cctggacaag cgcatcggcg acctgatcgc cttgcaacat tcggccatgc
     9781 agagcctgcc gaccatccgc atgatccagg ccaacaacca gatgctggtc gataaattcc
     9841 acaccatccg cgaaatcacc gtgccggcgt ggaagcggca attcatgctg gccttgagcc
     9901 tcaacgagca gaagaacgcc gtcgaactgg ccacggccat cgacgacacc accaacgacc
     9961 tgatgaagcg caatgcggcc ctgctgcatc gcacgtccgt cgaaacggcg aaggagaacc
    10021 aacgcctggt gatcgacgtg gacacgctca agcaggttca gacgacgctc atcaagaccg
    10081 tcgaggacgt tattcgcatc cagcaggaag gcgtgcagaa gcgcaaggat gccgagaagc
    10141 agatcgccgc aatgcgtggc gatcttcaag ccaagctgac ccgccagccc gtgcgcgagc
    10201 tggcccaaca ggagtccgta tgaatgccac aaacaccgat gttttcgccc aggtaggcgg
    10261 cctggaggcc cgaggcgcga agatgaagaa gcggggcacc cgcttcctca tcgcggcgct
    10321 ggcagtcctt gccattgccg ggatcggggc agtaacggga tgggcgatca gcccgagcgc
    10381 gacgcccgga agcattgacg tgccgcaggt gctggcatcg acattcagcg accaggtgcc
    10441 gggcagtgag ggcggcggcc tgggtggcgg cctgcccttc acttcggccg tcggggcatt
    10501 cacggacttc atggcggggc cggcaatttt taccttgggc attcttggca tagtggtcgc
    10561 gggtgccgtg ctcgtgttcg ggggtgagtt ctgcgggttc gtgcgatccg tctgcatgat
    10621 ggtgatagcc gtcagcatga ttttcgtgtc gtcgaacttg gtgaagggca ttctcggcgg
    10681 cgatcacgac gccggccctg cggagccttc gccgcgtgcg cgattcatgg cggccgtgga
    10741 ggccaaggat ttcgcgcgag tgcaagagct gatcgaggcg cgtggagcca agtcggcggc
    10801 tgattatgtc cttgcgcagc tcgccgtggc cgaaggtctg gaccgcaagc ctggtgcgcg
    10861 cgtcgtggtc gggaaagcgg cgggcagcat ggcaatgccg cctgcggcgc tgggttttac
    10921 gccaagggga gaagcggcat acgccatcga gcggtcagcc tatggtgagc cgaggtccag
    10981 cattgcgaag cagtaccagc aggaatggaa ccggaaggcg gcgacctggt gggcgatggc
    11041 cggtgtggcc ggcatcatcg gcgcgatcct ggcggcggcg gcaaccggct ttgttgggct
    11101 ggcagtgtcg atccgcaacc gagtgaagcg cgtgcgcgac ctgttggtga tggagccggg
    11161 tgcagagcca taagggatcg atccccctca agtcaaaagc ctccggtcgg aggcttttga
    11221 ctttctgctc gaggagctaa ttccattgag taagttttta agcacatcag cttcaaaagc
    11281 gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggtac ttcaagatcc
    11341 ccaattcgag ctcggtaccc ggggcggccg cttctaga
//
