LOCUS       sequence-404964-       11367 bp    DNA     circular SYN 15-APR-2025
DEFINITION  For Agrobacterium transformation. tagRFP flanked with an N-terminal 
            plastid transit peptide and a C-terminal FLAG tag under the control 
            of the cauliflower mosaic virus (CaMV) 35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11367)
  AUTHORS   Ishikawa K, Xie X, Osaki Y, Miyawaki A, Numata K, Kodama Y
  TITLE     Bilirubin is produced nonenzymatically in plants to maintain 
            chloroplast redox status.
  JOURNAL   Sci Adv. 2023 Jun 9;9(23):eadh4787. doi: 10.1126/sciadv.adh4787. 
            Epub 2023 Jun 7.
  PUBMED    37285441
REFERENCE   2  (bases 1 to 11367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-06-9"; volume: "9(23):eadh4787. doi"; pages: " 
            10.1126/sciadv.adh4787. Epub 2023 Jun 7"
COMMENT     Sequence Label: pGWB511-TP-tagRFP
FEATURES             Location/Qualifiers
     source          1..11367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1464..2174
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             2175..2198
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(2209..2233)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2244..2267
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2574..2590)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2574..2590)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2587..2609)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2598..2614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2622..2628,2629..2646,2647..2652))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2667..2688
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(2724..2976)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3127..4152)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(4204..4383)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    4889..4913
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5434..6225
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6471..7059
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6960..6979
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7213..7230
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7245..7385
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7311..7330)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7471..7493
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7729..7923
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7989..9062)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9491..10120)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagcag
     1201 gctttaaagg aaccaattca gtcgacatgg cttcctctat gctctcttcc gctactatgg
     1261 ttgcctctcc ggctcaggcc actatggtcg ctcctttcaa cggacttaag tcctccgctg
     1321 ccttcccagc cacccgcaag gctaacaacg acattacttc catcacaagc aacggcggaa
     1381 gagttaactg catgcaggtg tggcctccga ttggaaagaa gaagtttgag actctctctt
     1441 accttcctga ccttaccgat tccatggtgt ctaagggcga agagctgatt aaggagaaca
     1501 tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg
     1561 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
     1621 gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc agcagaacct
     1681 tcatcaacca cacccagggc atccccgact tctttaagca gtccttccct gagggcttca
     1741 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
     1801 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcccatcca
     1861 acggccctgt gatgcagaag aaaacactcg gctgggaggc caacaccgag atgctgtacc
     1921 ccgctgacgg cggcctggaa ggcagaaccg acatggccct gaagctcgtg ggcgggggcc
     1981 acctgatctg caacttcaag accacataca gatccaagaa acccgctaag aacctcaaga
     2041 tgcccggcgt ctactatgtg gaccacagac tggaaagaat caaggaggcc gacaaagaga
     2101 cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg
     2161 ggcacaaact taatgactac aaggatgacg atgacaaggg atatctagac ccagctttct
     2221 tgtacaaagt ggttgataac agcgactaca aggatgacga tgacaaggct tagagctcga
     2281 atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     2341 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     2401 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     2461 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     2521 tgtcatctat gttrytmsrk mrssaattgg ttccggaacc aattcgtaat catgtcatag
     2581 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2641 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattagg ctgaattagg
     2701 cgcgcctatt tctattaatt cccgatctag taacatagat gacaccgcgc gcgataattt
     2761 atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac
     2821 tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg
     2881 cttaacgtaa ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa
     2941 tcttaagaaa ctttattgcc aaatgtttga acgatcgggg aaattcgggg gatcgatccc
     3001 gcgaactgtg gacgagaact gtgccaccaa gcgtaaggcc gttctctcgc atttgccttg
     3061 ctaggctcgc gcgagttgct ggctgaggcg ttctcgaaat cagctcttgt tcggtcggca
     3121 tctactctat tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag
     3181 tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc
     3241 gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc
     3301 atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata
     3361 cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg
     3421 ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga
     3481 atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag
     3541 gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc
     3601 ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac
     3661 agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt
     3721 gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc
     3781 cgcagcgatc gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc
     3841 aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc
     3901 gctgaatgcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg
     3961 ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc
     4021 acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag
     4081 gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc
     4141 aggctttttc atatcttatt gccccccggg atcagatctg gattgagagt gaatatgaga
     4201 ctctaattgg ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat
     4261 atttgctagc tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt
     4321 atgtgcttag ctcattaaac tccagaaacc cgcggctgag tggctccttc aacgttgcgg
     4381 ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg
     4441 actcccttaa ttctccgctc atgatcttga tcccctgcgc catcagatcc ttggcggcaa
     4501 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     4561 caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc
     4621 actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta
     4681 gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt
     4741 cctttagcag cccttgcgcc ctgagtgctt gcggcagcgt gaagctgatc cgtcgagatt
     4801 aatagaaata aattcagcct aattcggcgt taattcagta cattaaaaac gtccgcaatg
     4861 tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca
     4921 gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat
     4981 cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac
     5041 cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg
     5101 gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aattcgggca
     5161 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     5221 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     5281 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     5341 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     5401 cagggcagtc gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta
     5461 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     5521 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat
     5581 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     5641 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     5701 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     5761 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     5821 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     5881 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     5941 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     6001 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     6061 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     6121 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     6181 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat
     6241 tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca
     6301 cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca
     6361 taataagccc tacacaaatt gggagatata tcatgcatga ccaaaatccc ttaacgtgag
     6421 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6481 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6541 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6601 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     6661 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6721 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6781 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6841 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6901 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6961 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7021 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7081 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7141 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7201 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     7261 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     7321 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     7381 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     7441 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     7501 cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc
     7561 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
     7621 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
     7681 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
     7741 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
     7801 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
     7861 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
     7921 acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
     7981 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
     8041 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
     8101 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
     8161 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
     8221 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
     8281 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
     8341 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
     8401 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
     8461 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
     8521 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
     8581 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
     8641 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
     8701 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
     8761 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
     8821 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct
     8881 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
     8941 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
     9001 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
     9061 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc
     9121 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
     9181 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
     9241 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
     9301 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
     9361 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
     9421 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
     9481 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
     9541 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
     9601 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
     9661 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
     9721 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
     9781 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
     9841 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
     9901 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
     9961 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
    10021 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
    10081 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
    10141 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
    10201 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
    10261 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac
    10321 gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt
    10381 aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt
    10441 tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg
    10501 cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc
    10561 gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc
    10621 agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg
    10681 cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg
    10741 aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg
    10801 gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg
    10861 tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg
    10921 ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg
    10981 atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc
    11041 aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg
    11101 ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac
    11161 gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg
    11221 tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag
    11281 cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca
    11341 catacaaatg gacgaacgga taaacct
//
