LOCUS       sequence-405614-        2069 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Level 0 plasmid containing 3 consecutive stop codons with DE 
            overhangs used to build a level 1 constuct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_DE-3xStop
FEATURES             Location/Qualifiers
     source          1..2069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1207..1795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1287..1306)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1886..2069,1..608))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
       61 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
      121 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
      181 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
      241 tcgaagatac cagcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
      301 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
      361 agaatctcac tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
      421 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc
      481 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
      541 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
      601 tccctcatgc gaaacgatcc tcatcctgtc tcttgatcag atattgatcc cctgcgccat
      661 cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca
      721 gagggcgccc cagctggcaa ttccggttcg cttgctaagc ttgcatgcct gcaagtcgac
      781 tctagaggag cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
      841 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
      901 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
      961 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctcattact cgcatccatt
     1021 ctcaggctgt ctcgtctcgt ctcaggtctc caggttaatg ataggcttcg agaccaccag
     1081 gatacataga ttaccacaac tccgagccct tccacccaca gaatcagggg ataacgcagg
     1141 aaagaacatg tgagcaaaag gccagcaaaa ggcaaggaac cgtaaaaagg ccgcgttgct
     1201 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1261 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1321 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1381 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1441 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1501 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1561 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1621 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1681 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1741 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1801 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1861 tttggtcatg cattctaggt gattattatt tgccgactac cttggtgatc tcgcctttca
     1921 cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc
     1981 caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca
     2041 ttgcccagtc ggcagcgaca tccttcggc
//
