LOCUS       sequence-405616-        2735 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Level 0 plasmid containing a RFP1 fluorescent protein with DE 
            overhangs used to build a level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_DE-RFP1
FEATURES             Location/Qualifiers
     source          1..2735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             577..1251
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     rep_origin      complement(1394..1982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1474..1493)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2073..2735,1..129))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag
       61 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc
      121 ttccctcatg cgaaacgatc ctcatcctgt ctcttgatca gatattgatc ccctgcgcca
      181 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc
      241 agagggcgcc ccagctggca attccggttc gcttgctaag cttgcatgcc tgcaagtcga
      301 ctctagagga gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
      361 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
      421 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
      481 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctcattac tcgcatccat
      541 tctcaggctg tctcgtctcg tctcaggtct caaggtgctt cctccgaaga cgttatcaaa
      601 gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc
      661 gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc
      721 aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc
      781 aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa
      841 ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag
      901 gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc
      961 ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt
     1021 atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac
     1081 ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg
     1141 ccgggtgctt acaaaaccga catcaaactg gacataacct cccacaacga agactacacc
     1201 atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta agcttagaga
     1261 ccaccaggat acatagatta ccacaactcc gagcccttcc acccacagaa tcaggggata
     1321 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1381 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1441 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1501 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1561 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1621 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1681 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1741 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1801 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1861 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1921 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1981 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2041 aagggatttt ggtcatgcat tctaggtgat tattatttgc cgactacctt ggtgatctcg
     2101 cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct
     2161 tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg
     2221 cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg
     2281 taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc
     2341 gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga
     2401 tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc
     2461 agcaagatag ccagatcaat gtcgatcgtg gctggctcga agataccagc aagaatgtca
     2521 ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg
     2581 tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcactctc tccaggggaa
     2641 gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg
     2701 gtcaccgtaa ccagcaaatc aatatcactg tgtgg
//
