LOCUS       sequence-405700-        2472 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 41316 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0_EF-acc2
FEATURES             Location/Qualifiers
     source          1..2472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             792..1583
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1674..2262
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2163..2182
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atttcaatgt ggtgcgagta ggggatatgg gaatgggtga ccctctaaga caactgtcgg
       61 aatcgccccc ttaacatcaa cagtaaagga acgttcttgc gtcgactctg gtatgtattg
      121 gctgtgaagc acgcgaacaa ccttttccta cgccgcttcg tcaacgacgg cttagtgtgc
      181 tggttttgag gaaagatccg tttctgttcc agacttgatt cacgttctgt gaacacaaac
      241 atcgctcttg cgtttaaatg gcattgagaa acaacctctg attaaacaag ccactgactg
      301 taaaataata tttaagacgt agttggtccc ggggggttat gcttaagcgg agacctgaga
      361 cgagacgaga cagcctgaga atggatgcga gtaatgagct cggcacaaaa tcaccactcg
      421 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga
      481 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac
      541 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg
      601 tgatcaccgc tcctctagag tcgacttgca ggcatgcaag cttagcaagc gaaccggaat
      661 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
      721 tcttgccgcc aaggatctga tggcgcaggg gatcaatatc tgatcaagag acaggatgag
      781 gatcgtttcg catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
      841 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
      901 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
      961 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     1021 ctggagagag tgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     1081 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     1141 ttcttgctgg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     1201 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     1261 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     1321 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     1381 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     1441 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     1501 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     1561 tcaccaaggt agtcggcaaa taataatcac ctagaatgca tgaccaaaat cccttaacgt
     1621 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1681 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1741 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     1801 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     1861 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1921 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1981 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2041 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2101 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2161 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2221 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2281 tttttacggt tccttgcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2341 cctgattctg tgggtggaag ggctcggagt tgtggtaatc tatgtatcct ggtggtctcg
     2401 agcgtccact tgacggaaac cgtatcaacg cgtctgccta ttcatcgctg agcaaggaga
     2461 catggaactt tc
//
