LOCUS       sequence-405716-        9705 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of Sox13 with Sox2 DBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Price RM, Budzynski MA, Shen J, Mitchell JE, Kwan JZJ, Teves SS
  TITLE     Heat shock transcription factors demonstrate a distinct mode of 
            interaction with mitotic chromosomes.
  JOURNAL   Nucleic Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304.
  PUBMED    37114996
REFERENCE   2  (bases 1 to 9705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304."
COMMENT     Sequence Label: Halo-S13S2DBD
FEATURES             Location/Qualifiers
     source          1..9705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        110..321
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             334..602
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             634..699
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             709..1599
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             1612..1632
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     misc_feature    3499..4071
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3666..3683)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3893..3912
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4080..4874
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4134..4153)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4744..4763
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4934..4954)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    4996..5117
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5033..5052)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5087..5106
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5880..5914
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6405..6421)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6405..6421)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6418..6440)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6429..6445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6453..6459,6460..6477,6478..6483))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6498..6519
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6636..6653)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6807..7395)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6887..6906)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7566..8357,8358..8426))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8189..8208
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8427..8531)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8557..9012)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8694..8715)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8906..8925
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9139..9161
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9153..9170
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9154..9170
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   9300..9362
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg ggatacgggg
       61 aaaaggcctc cacggccaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
      121 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
      181 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
      241 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
      301 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
      361 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
      421 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
      481 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
      541 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
      601 tccaagctgt gaccggcgcc tactctagcc atggactaca aagaccatga cggtgattat
      661 aaagatcatg acatcgatta caaggatgac gatgacaagc ttggctccat ggcagaaatc
      721 ggtactggct ttccattcga cccccattat gtggaagtcc tgggcgagcg catgcactac
      781 gtcgatgttg gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa cccgacctcc
      841 tcctacgtgt ggcgcaacat catcccgcat gttgcaccga cccatcgctg cattgctcca
      901 gacctgatcg gtatgggcaa atccgacaaa ccagacctgg gttatttctt cgacgaccac
      961 gtccgcttca tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt
     1021 cacgactggg gctccgctct gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa
     1081 ggtattgcat ttatggagtt catccgccct atcccgacct gggacgaatg gccagaattt
     1141 gcccgcgaga ccttccaggc cttccgcacc accgacgtcg gccgcaagct gatcatcgat
     1201 cagaacgttt ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct gactgaagtc
     1261 gagatggacc attaccgcga gccgttcctg aatcctgttg accgcgagcc actgtggcgc
     1321 ttcccaaacg agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa
     1381 tacatggact ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg caccccaggc
     1441 gttctgatcc caccggccga agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct
     1501 gtggacatcg gcccgggtct gaatctgctg caagaagaca acccggacct gatcggcagc
     1561 gagatcgcgc gctggctgtc gacgctcgag atttccggcg agccaaccac tgaggatctg
     1621 tactttcaga gcgataactc tagatccatg cagagccccg tctctgtgca gctggcccca
     1681 gacagcgcta gcaccatggt gaactgcacc atcaagtctg aggagaagaa ggagccttgc
     1741 cacgaggctc cccaaggtgc agcccctgca gtggagaccc agcctggaga cccggccctg
     1801 gcctctcagg atgccaccaa cgctaaagct cctcctcagg actgtgcctc accagagagc
     1861 agtgggtccc cagaacccaa gagaccagca gcttccgagg ctgcttcggg aagccaggag
     1921 cgcctggact tcaaccgcaa cttacaggag gttgtgccag ccattgagaa gctgctgtcc
     1981 agtgactgga aggagaggtt tctgggaaga agcaatgtgg aagctaagga tgtcaaaggg
     2041 acccaagaga gcctggctga gaaggaactc cagcttttgg tcatgatcca tcagctctct
     2101 gcccttcggg accagctcct tacggcccac tctgagcaga agaacatggc cgccatgctg
     2161 tttgagaagc agcagcaaca gatggaactg gccaggcagc agcaggagca gatcgccaag
     2221 cagcagcagc agctgattca acagcagcac aagatcaacc tcctgcagca gcagatccag
     2281 caggttaaca tgccttacgt catgatccca gccttccccc caagccacca gcctttgcct
     2341 gtcactcctg actcccagct ggctttacct attcagccca ttccctgcaa gccagtggaa
     2401 tacccactgc agctgctgca cagcccgcct gcccctgtgg tgaagaggtc gggggtggcc
     2461 gcccaccacc ccctgcagga gcccccacaa ccactgaacc tcacagccaa gcccaaggta
     2521 cctgagctgc ccaacacctc cagctctccc agcctgaaga tgaacagttg tgggcctcgc
     2581 cccgccagcc atggggctcc cacaagggac ctgcaatcca gcccccccag cctgcctctg
     2641 ggcttcctcg gtgaagggga cgccgtcacc aaagccatcc aggatgcccg gcagctgctg
     2701 cacagccaca gtggggcttt ggaaaactcc cccaataccc cattccggaa ggacctcatc
     2761 agcctggact cctccccggc caaggagcgg ctggaggaga gctgtgtgca tcccctggag
     2821 gaagccatgc tgagctgtga catggatggc tcccgtcatt tttccgagtc tcggaacagc
     2881 agccacgtca agaggcccat gaacgccttc atggtatggt cccgggggca gcggcgtaag
     2941 atggcccagg agaaccccaa gatgcacaac tcggagatca gcaagcgcct gggcgcggag
     3001 tggaaacttt tgtctgagac cgagaagcgg ccgttcatcg acgaggccaa gcggctgcgc
     3061 gctctgcaca tgaaggagca cccggattat aaatacaagc caaggcctaa gcgtacctgt
     3121 gtcgtggagg gccggcggct gcgagtgggc gagtacaagg ccttgatgag gacacggcgc
     3181 cagggtgctc gccaaagcta caccatcccc ccgcaggctg gccaggcaca ggtgagctca
     3241 gatatcctgt tccctcgggc agcgggcttg cccctggcac gtccactggt ggagcactat
     3301 gacccccagg gcctggaccc caacatgcct gtcatcatca atacctgcag tctccgggag
     3361 gaaggggagg gcacagatga caggcactcg gtggccgatg gagagatgta ccggtacagc
     3421 gaggacgagg actcagaggg tgacgagaag agtgatgagg agctggtggt actcacagac
     3481 tgagcggccg cggccgcgcc cctctccctc ccccccccct aacgttactg gccgaagccg
     3541 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     3601 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     3661 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     3721 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     3781 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     3841 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     3901 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     3961 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     4021 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgataat acctccggaa
     4081 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     4141 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     4201 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     4261 aggacgaggc agcgcggcta tcgtggctgg ccgcgacggg cgttccttgc gcagctgtgc
     4321 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     4381 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     4441 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     4501 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     4561 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     4621 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     4681 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     4741 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     4801 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     4861 acgagttctt ctgagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     4921 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4981 atcatgcgtt aactaaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5041 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5101 ctcatcaatg tatcttatca tgtctggaat tgactcaaat gatgtcaatt agtctatcag
     5161 aagctcatct ggtctccctt ccgggggaca agacatccct gtttaatatt taaacagcag
     5221 tgttcccaaa ctgggttctt atatcccttg ctctggtcaa ccaggttgca gggtttcctg
     5281 tcctcacagg aacgaagtcc ctaaagaaac agtggcagcc aggtttagcc ccggaattga
     5341 ctggattcct tttttagggc ccattggtat ggctttttcc ccgtatcccc ccaggtgtct
     5401 gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg
     5461 tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag
     5521 cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg
     5581 ggggctttgg gggggggctg tccctgatat ctataacaag aaaatatata tataataagt
     5641 tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa atcttaaaag
     5701 tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt tcaaaatcag
     5761 tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga ctgagatgtc
     5821 ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc
     5881 atgcgtcaat tttacgcaga ctatctttct agggttaatc tagctgcatc aggatcatat
     5941 cgtcgggtct tttttccggc tcagtcatcg cccaagctgg cgctatctgg gcatcgggga
     6001 ggaagaagcc cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt ttgccgagga
     6061 tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt accatgatga ttcgggaagg
     6121 tgtggccatg cacgccttta acggtgaact gttcgttcag gccacctggg ataccagttc
     6181 gtcgcggctt ttccggacac agttccggat ggtcagcccg aagcgcatca gcaacccgaa
     6241 caataccggc gacagccgga actgccgtgc cggtgtgcag attaatgaca gcggtgcggc
     6301 gctgggatat tacgtcagcg aggacgggta tcctggctgg atgccgcaga aatggacatg
     6361 gataccccgt gagttacccg gcgggcgcgc ttggcgtaat catggtcata gctgtttcct
     6421 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6481 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6541 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6601 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6661 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6721 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6781 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6841 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6901 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6961 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7021 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7081 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7141 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7201 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7261 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7321 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7381 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7441 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7501 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7561 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7621 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7681 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7741 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7801 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7861 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7921 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7981 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8041 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8101 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8161 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8221 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8281 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8341 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8401 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8461 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8521 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     8581 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     8641 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     8701 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     8761 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     8821 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     8881 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     8941 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     9001 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     9061 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     9121 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcct
     9181 cgttcattca cgtttttgaa cccgtggagg acgggcagac tcgcggtgca aatgtgtttt
     9241 acagcgtgat ggagcagatg aagatgctcg acacgctgca gaacacgcag ctagattaac
     9301 cctagaaaga taatcatatt gtgacgtacg ttaaagataa tcatgtgtaa aattgacgca
     9361 tgtgttttat cggtctgtat atcgaggttt atttattaat ttgaatagat attaagtttt
     9421 attatattta cacttacata ctaataataa attcaacaaa caatttattt atgtttattt
     9481 atttattaaa aaaaacaaaa actcaaaatt tcttctataa agtaacaaaa cttttatgag
     9541 ggacagcccc cccccaaagc ccccagggat gtaattacgt ccctcccccg ctagggggca
     9601 gcagcgagcc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc
     9661 agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcg
//
