LOCUS       sequence-405797-        5529 bp    DNA     circular SYN 15-APR-2025
DEFINITION  AAV plasmid expressing deltion and mutant TXNIP in retinal pigment 
            epithelium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Xue Y, Zhou Y, Cepko CL
  TITLE     Txnip deletions and missense alleles prolong the survival of cones 
            in a retinitis pigmentosa mouse model.
  JOURNAL   Elife. 2024 May 10;12:RP90749. doi: 10.7554/eLife.90749.
  PUBMED    38727583
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2024-05-10"; volume: "12:RP90749. doi"; pages: " 
            10.7554/eLife.90749"
COMMENT     Sequence Label: AAV-Best1-C.Txnip(C247S)
FEATURES             Location/Qualifiers
     source          1..5529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   259..388
                     /label=AAV2 ITR
     intron          1084..1216
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1199..1218
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(2040..2060)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2458..2469)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2536..2553)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2542..2749
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2837..2966
                     /label=AAV2 ITR
     primer_bind     complement(2985..3002)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2985..3001)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2994..3016)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3143..3598
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3230..3249)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3440..3461
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3624..3728
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3729..3797,3798..4589)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3947..3966)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4760..5348
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5249..5268
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      421 tctacgtagc catgctctag gaagatcgga attcgccctt aagctagctc cttttcagat
      481 aagggcactg aggctgagag aggagctgaa acctacccgg ggtcaccaca cacaggtggc
      541 aaggctggga ccagaaacca ggactgttga ctgcagcccg gtattcattc tttccatagc
      601 ccacagggct gtcaaagacc ccagggccta gtcagaggct cctccttcct ggagagttcc
      661 tggcacagaa gttgaagctc agcacagccc cctaaccccc aactctctct gcaaggcctc
      721 aggggtcaga acactggtgg agcagatcct ttagcctctg gattttaggg ccatggtaga
      781 gggggtgttg ccctaaattc cagccctggt ctcagcccaa caccctccaa gaagaaatta
      841 gaggggccat ggccaggctg tgctagccgt tgcttctgag cagattacaa gaagggacta
      901 agacaaggac tcctttgtgg aggtcctggc ttagggagtc aagtgacggc ggctcagcac
      961 tcacgtgggc agtgccagcc tctaagagtg ggcaggggca ctggccacag agtcccaggg
     1021 agtcccacca gcctagtcgc cagacccaga tcctgcagaa gttggtcgtg aggcactggg
     1081 caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg
     1141 agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt
     1201 gcctttctct ccacaggtgt ccaggcatgc tagtggatgt caatacccct gacttaatgg
     1261 caccagtgtc tgccaaaaag gagaagaaag tttcctgcat gttcattcct gatggacgtg
     1321 tgtcagtctc tgctcgaatt gaccgaaaag gattctgtga aggtgatgac atctccatcc
     1381 atgctgactt tgagaacacg tgttcccgaa tcgtggtccc caaagcggct attgtggccc
     1441 gacacactta ccttgccaat ggccagacca aagtgttcac tcagaagctg tcctcggtca
     1501 gaggcaatca cattatctca gggactagcg catcgtggcg tggcaagagc ctcagagtgc
     1561 agaagatcag accatccatc ctgggctgca acatcctcaa agtcgaatac tccttgctga
     1621 tctacgtcag tgtccctggc tccaagaaag tcatccttga tctgccccta gtgattggca
     1681 gcaggtctgg tctgagcagc cggacatcca gcatggccag ccggacgagc tctgagatga
     1741 gctggataga cctaaacatc ccagataccc cagaagctcc tccttgctat atggacatca
     1801 ttcctgaaga tcacagacta gagagcccca ccacccctct gctggacgat gtggacgact
     1861 ctcaagacag ccctatcttt atgtacgccc ctgagttcca gttcatgccc ccacccactt
     1921 acactgaggt ggatccgtgc gtccttaaca acaacaacaa caacaacaac gtgcagtagt
     1981 ggatccaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2041 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2101 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     2161 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     2221 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     2281 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     2341 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     2401 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     2461 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     2521 cgtcttcgag atctgcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     2581 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     2641 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     2701 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggac tcgagttaag
     2761 ggcgaattcc cgataaggat cttcctagag catggctacg tagataagta gcatggcggg
     2821 ttaatcatta actacaagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     2881 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     2941 gcctcagtga gcgagcgagc gcgcagcctt aattaaccta attcactggc cgtcgtttta
     3001 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     3061 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     3121 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     3181 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     3241 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     3301 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     3361 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     3421 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     3481 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     3541 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     3601 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3661 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3721 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     3781 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     3841 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     3901 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     3961 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4021 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4081 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4141 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4201 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4261 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4321 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4381 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4441 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4501 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4561 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4621 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4681 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4741 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4801 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4861 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     4921 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4981 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5041 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5101 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5161 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5221 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5281 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5341 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5401 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5461 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5521 aagcggaag
//
