LOCUS       sequence-405799-        5463 bp    DNA     circular SYN 15-APR-2025
DEFINITION  AAV plasmid expressing deltion TXNIP in retinal pigment epithelium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Xue Y, Zhou Y, Cepko CL
  TITLE     Txnip deletions and missense alleles prolong the survival of cones 
            in a retinitis pigmentosa mouse model.
  JOURNAL   Elife. 2024 May 10;12:RP90749. doi: 10.7554/eLife.90749.
  PUBMED    38727583
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2024-05-10"; volume: "12:RP90749. doi"; pages: " 
            10.7554/eLife.90749"
COMMENT     Sequence Label: AAV-Best1-N.Txnip
FEATURES             Location/Qualifiers
     source          1..5463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   259..388
                     /label=AAV2 ITR
     intron          1084..1216
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1199..1218
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(1974..1994)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2392..2403)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2470..2487)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2476..2683
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2771..2900
                     /label=AAV2 ITR
     primer_bind     complement(2919..2936)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2919..2935)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2928..2950)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3077..3532
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3164..3183)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3374..3395
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3558..3662
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3663..3731,3732..4523)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3881..3900)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4694..5282
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5183..5202
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      421 tctacgtagc catgctctag gaagatcgga attcgccctt aagctagctc cttttcagat
      481 aagggcactg aggctgagag aggagctgaa acctacccgg ggtcaccaca cacaggtggc
      541 aaggctggga ccagaaacca ggactgttga ctgcagcccg gtattcattc tttccatagc
      601 ccacagggct gtcaaagacc ccagggccta gtcagaggct cctccttcct ggagagttcc
      661 tggcacagaa gttgaagctc agcacagccc cctaaccccc aactctctct gcaaggcctc
      721 aggggtcaga acactggtgg agcagatcct ttagcctctg gattttaggg ccatggtaga
      781 gggggtgttg ccctaaattc cagccctggt ctcagcccaa caccctccaa gaagaaatta
      841 gaggggccat ggccaggctg tgctagccgt tgcttctgag cagattacaa gaagggacta
      901 agacaaggac tcctttgtgg aggtcctggc ttagggagtc aagtgacggc ggctcagcac
      961 tcacgtgggc agtgccagcc tctaagagtg ggcaggggca ctggccacag agtcccaggg
     1021 agtcccacca gcctagtcgc cagacccaga tcctgcagaa gttggtcgtg aggcactggg
     1081 caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg
     1141 agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt
     1201 gcctttctct ccacaggtgt ccaggcatgg tgatgttcaa gaagatcaag tcttttgagg
     1261 tggtcttcaa cgaccccgag aaagtgtacg gcagcgggga gaaggtggcc ggacgggtaa
     1321 ttgtggaagt gtgtgaagtt acccgagtca aagctgtcag gatcctggct tgcggcgtgg
     1381 ccaaggtcct gtggatgcaa gggtctcagc agtgcaaaca gactttggac tacttgcgct
     1441 atgaagacac acttctccta gaagagcagc ctacagcagg tgagaacgag atggtgatca
     1501 tgaggcctgg aaacaaatat gagtacaagt tcggcttcga gcttcctcaa gggcccctgg
     1561 gaacatcctt taaaggaaaa tatggttgcg tagactactg ggtgaaggct tttctcgatc
     1621 gccccagcca gccaactcaa gaggcaaaga aaaacttcga agtgatggat ctagtggatg
     1681 tcaatacccc tgacttaatg gcaccagtgt ctgccaaaaa ggagaagaaa gtttcctgca
     1741 tgttcattcc tgatggacgt gtgtcagtct ctgctcgaat tgaccgaaaa ggattctgtg
     1801 aaggtgatga catctccatc catgctgact ttgagaacac gtgttcccga atcgtggtcc
     1861 ccaaagcggc tattgtggcc cgacacactt accttgccaa tggccagacc taatggatcc
     1921 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     1981 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     2041 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     2101 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     2161 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     2221 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     2281 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     2341 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     2401 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     2461 cgagatctgc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     2521 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     2581 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     2641 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggactcgagt taagggcgaa
     2701 ttcccgataa ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc
     2761 attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     2821 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     2881 gtgagcgagc gagcgcgcag ccttaattaa cctaattcac tggccgtcgt tttacaacgt
     2941 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3001 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     3061 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3121 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3181 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3241 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3301 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3361 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     3421 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3481 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     3541 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3601 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3661 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     3721 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     3781 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     3841 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     3901 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     3961 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4021 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4081 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4141 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4201 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4261 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     4321 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     4381 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4441 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     4501 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     4561 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4621 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4681 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4741 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4801 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     4861 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4921 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4981 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5041 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5101 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5161 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5221 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5281 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5341 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5401 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5461 aag
//
