LOCUS       sequence-405836-       13669 bp    DNA     circular SYN 14-APR-2025
DEFINITION  FExpress phiC31 integrase under heat shock control. Insert marked 
            with 3XP3-EGFP, which expresses in eyes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13669)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 13669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT56
FEATURES             Location/Qualifiers
     source          1..13669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             126..917
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1954..1972
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1954..1971
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        2049..2469
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     protein_bind    2476..2599
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2622..2627,2628..2645,2646..2652)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        2695..2799
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2800..2868,2869..3660)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3018..3037)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     4181..4200
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4340..4463)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      4752..4761
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4758..4760,4761..4763,4764..5477)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4758..4760,4761..4763,4764..5474)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4803..4824)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5064..5083)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5411..5432
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5600..5721
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5637..5656)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5691..5710
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5748..5764
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10304..10427
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(10450..10455,10456..10473,10474..10480)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             10534..11193
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(10600..10619)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        11202..11647
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(11648..11734,11735..13069)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     protein_bind    complement(13108..13231)
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(13323..13342)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13324..13342)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
       61 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
      121 acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
      181 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
      241 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
      301 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
      361 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
      421 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
      481 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
      541 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
      601 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
      661 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
      721 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
      781 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
      841 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
      901 aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg
      961 cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg
     1021 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata
     1081 tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaaatcgt cacctgcagg aaggtttaaa cgcatttagg tgacactata gaagtgtgta
     1981 tcgctcgagg gatccgaatt cgaagtcttg gtacggaggg gatccgatcg acaagccgaa
     2041 catatgggtt ttttgctcac ctgtgattgc tcctactcaa atacaaaaac atcaaatttt
     2101 ctgtcaataa agcatattta tttatattta ttttacagga aagaattcct tttaaagtgt
     2161 attttaacct ataatgaaaa acgattaaaa aaaatacata aaataattcg aaaatttttg
     2221 aatagcccag gttgataaaa attcatttca tacgttttat aacttatgcc cctaagtatt
     2281 ttttgaccat agtgtttcaa ttctacatta attttacaga gtagaatgaa acgccaccta
     2341 ctcagccaag aggcgaaaag gttagctcgc caagcagaga gggcgccagt gctcactact
     2401 ttttataatt ctcaacttct ttttccagac tcagttcgta tatatagacc tattttcaat
     2461 ttaacgtcgg ggcctcaact ttgtataata aagttgaacg agaaacgtaa aatgatataa
     2521 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac
     2581 aacatatcca gtcactatgg gccgcattag gcaccccagg ctttacactt tatgcttccg
     2641 gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga gctacgcgga
     2701 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2761 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2821 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2881 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2941 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3001 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3061 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3121 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3181 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3241 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3301 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3361 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3421 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3481 tttattgctg ataaatctgg agccggtgag cgtgggtccc gcggtatcat tgcagcactg
     3541 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3601 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3661 tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
     3721 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag
     3781 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
     3841 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
     3901 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
     3961 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat
     4021 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat cgatacaccg
     4081 ggccgtcgca tggttattcc acttgcgtct gcgcgcttgc tctcagataa agtctcccgt
     4141 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     4201 gccagtgtgc cggtttccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     4261 gacatcaaaa acgccattaa cctgatgttc tggggaatat aatgtcaggc tcccttatac
     4321 acagccagtc tgcacgtcgc atagtgactg gatatgttgt gttttacagt attatgtagt
     4381 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     4441 ttcaactttt ctatacaaag ttgtccagct ggagcaatgg ttaattcgag ctcgcccggg
     4501 gatctaattc aattagagac taattcaatt agagctaatt caattaggat ccaagcttat
     4561 cgatttcgaa ccctcgaccg ccggagtata aatagaggcg cttcgtctac ggagcgacaa
     4621 ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc
     4681 gcagctgaac aagctaaaca atcggggtac cgctagagtc gacggtaccg cgggcccggg
     4741 atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     4801 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     4861 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     4921 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     4981 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     5041 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     5101 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     5161 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     5221 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     5281 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     5341 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     5401 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     5461 acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt
     5521 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     5581 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     5641 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5701 caaactcatc aatgtatctt aaagcttatc gatacgcgta cggcgcgcga ggtcgacggt
     5761 atcgataagc ttgatatcga attcctgcag ctggttgtag gtgcagttgc gcttcttgca
     5821 cttggcgcac ttgaggagat cggtcttggt gccctgcacg gtggccagct gggcatcgtt
     5881 gatggcctcc ttgacgaact tctcgcgcag cttcttcatc tcgtcgctgg ccatctcctc
     5941 cggcgtcatt ttggccagct gcttggcagt gacggcgccg cacataaagt tgccgcgcaa
     6001 tccaggattc ttggggtcct tcagattggc cacgcgtgac ctaatgcgat tcttgtactt
     6061 catatccgtg ttattgaact cggagtaaat ggcatcctcc agttcggcgg ccatttcctc
     6121 cggctcaccg catccctcgg gcacttcgcc aatcttcagt gcggtggcca gcatttcgcg
     6181 gcatttgatg cggaccgcat ctgtcatgcc accggaggga aacgaggtct gtgacgagga
     6241 tgtcgagcct ttcttctctt tatccttgga tgagctggat gaactggact tatccttgcc
     6301 ggagattgac gacgacgact tggcggcact ggtggacttc gaggcactgc tgttgttgga
     6361 ggagccctcc ttagcggagc tgttgttcgg tgtggttggc gccgggctgg ccaggaagcg
     6421 tttccagttc ttgatcagcg tcttggccag agcgatcacc tcgtcgtcct tgctactctt
     6481 gcgcagctcg tttacggtca tgccgatgcg cgttttggtc agaatgtcga gattgatgtt
     6541 aagcgtttgc agggccttca gcaggtccag agcctgatcc tgtccctgta cggtggatat
     6601 atgtatattt gtgacatgat gcgcggcacg ttagctaatc ccgatactta ctgtgccgtc
     6661 gctggccatc ttgctcatct tcttttggat tcgaaacact tcctcctcca cgctcatttt
     6721 ggcccactta atttgatcag aggttaggtt aactggcgtc ggctgtcggc tttaatcggt
     6781 gctgcgggtc tgcgacagtt gtttatgcgg aagccgggct atttcttttt ttccctccaa
     6841 taaatgttgt ttactatttt tttcgtttct gtattcgcag aacaagttga cagaacaccc
     6901 taacgcgttt tagtgttacc gggcggtggc ttgtcaagaa ataccttccc atggctaata
     6961 caattcccaa ctggaagtag tttgggtttc agaagtcaac agtgttaccg tattttaatg
     7021 tacatctggc ttttaagcga gtacacacac atagcattgc gatgtttttt aaacttgcac
     7081 tgcttgcaag caggggtcca ctgtatttca atgaaagaac agcgtgttgg gcgtttttga
     7141 attatactaa ttaccttcaa taacaattcc ctatatatca cttagtttta ataaataaat
     7201 cgtttgagtg tggtctagag atgatttatt atgatgaagt gcggttgttg gggacaagcg
     7261 ggatacgtct gcgggtcaaa tcggcggtgg gacatagtga gagggcaacg gcgaacaatc
     7321 accaacgcgc cctggtacgc gagaaatgtt gatatcgcac atgacctcag catccacctt
     7381 cagtttgaga acaaattaaa tcaggcacat accagacaac caggagaaca ctgaggaggc
     7441 gtcatcaaag atatctttgg acacgtggca taaacaagcc aaactattat ctaatcaata
     7501 tttttataca aattttgtat gtttccatgt tatcttcgtt gctcctgtta gttactacgt
     7561 ttcatagccc ttaaattggt tgcttaacgt aaaataaaat tatattgtat aaaaataaag
     7621 aggaattcac ctataacaaa cgaaaaagta aataaataag aacttcccca tgttaaaaat
     7681 gccaccacca tctttacata taagtatgta catatgaatg aatcacttag gttgcttgaa
     7741 tattacaatt tcattaatag ctaaatcaca tttgatgtgt tagtggaaaa cggctatata
     7801 tataaattat cgaaattgtg aatatcgaat tgcgatagca caatgggaaa ttccaccact
     7861 agatttttgg tacttttaac agatcctttt cggttttgcg ttgcgcgaag tgatctgaac
     7921 ttatcaaagt ttgtaaggta atacataaag tgaaaaagaa ttaatttgct cttgaaaggc
     7981 aggccaaatt aaaaaaaaaa tatcaatatg gacacgtatg ccggtgctta cgaccgtcag
     8041 agccgcgagc gggaaaattc cagcgcagca agtccagcca cacagcgtag cgccaacgag
     8101 gacaaggcgg ccgacttgca gcgagaagtc gagcgagatg gtggcaggtt caggttcgtc
     8161 gggcatttct ccgaagctcc aggcactagc gcgttcggaa cagccgagcg cccggagttc
     8221 gaacgcatcc tgaacgagtg ccgtgccggc aggctcaaca tgatcatagt ctatgacgtg
     8281 agtcgcttct cgcgcctgaa ggtcatggac gcgattccga ttgtgagcga attgctcgcc
     8341 ctgggtgtga cgatagtgtc cacgcaggaa ggcgtgtttc ggcagggaaa cgtcatggac
     8401 ctgatacacc tgattatgcg cttggacgct tcgcacaagg agtccagcct gaagagtgcg
     8461 aagattctcg acaccaagaa cctgcagcgc gaattgggcg gttatgtcgg cggtaaggct
     8521 ccctacggct tcgagcttgt ttcggagacg aaggagatca cgcgtaacgg ccgaatggtc
     8581 aatgtcgtga tcaacaagct tgcccactcg accacacccc ttacccgacc cttcgagttc
     8641 gagcccgacg ttatccgttg gtggtggcgt gagatcaaga cccacaagca ccttcccttc
     8701 aagccaggca gtcaagccgc cattcacccg ggcagcatta cggggctgtg taagcgcatg
     8761 gacgcagatg ctgtgccaac aaggggtgag acgattggca agaagaccgc ttcaagcgcc
     8821 tgggaccccg caaccgttat gcgaatcctt cgtgacccgc gtattgcagg ctttgccgct
     8881 gaggtgatct acaagaagaa gccggatgga acgccgacca cgaagataga gggttaccgc
     8941 attcagcgcg atccgatcac cttgcgcccc gtggagctcg attgcggacc catcatcgag
     9001 cccgctgagt ggtatgagct gcaggcgtgg ttggatggca ggggtcgcgg caagggtctt
     9061 tcccggggac aagccatact gagcgccatg gacaagctgt actgcgaatg tggcgccgtg
     9121 atgacttcga agcgcggcga ggaatcgatc aaggactcct accgctgccg tcgacggaag
     9181 gtggttgacc caagcgcacc tggtcagcat gagggcacgt gcaacgtgag catggcggca
     9241 ctcgacaagt tcgttgcgga acgcatcttc aacaagatca ggcacgctga aggcgacgaa
     9301 gagacgttgg cccttctgtg ggaagccgcg cgacgcttcg gcaagctcac tgaggcgcca
     9361 gagaagtccg gcgaacgggc gaaccttgtt gcggagcgag ccgatgcctt gaacgccctc
     9421 gaagagctgt acgaagaccg cgctgcagga gcttacgacg gacccgttgg caggaagcac
     9481 ttccggaagc aacaggcagc gctgacactc cggcagcaag gagcagaaga gcgacttgcc
     9541 gagcttgaag ccgccgaagc cccaaagctc cccttggacc aatggttccc cgaagacgcc
     9601 gacgctgacc caactggacc gaagtcgtgg tggggacgcg catcagtgga cgacaagcgc
     9661 gtgtttgtgg gcctcttcgt ggacaagatc gttgtgacca agagcactac cggcagggga
     9721 cagggaacac ccatcgagaa gcgcgcttcg atcacgtggg caaagccccc gaccgatgac
     9781 gatgaagatg acgcccagga tggcaccgaa gacgtagcgg cctagctcga gaatgtatgg
     9841 acatagattt caaataatta aatgtaatgc agtaattgat gtaattagtt aaataagtta
     9901 gatattaata acatattaat tatatgtatt ataacgcata taataataaa atgcatattt
     9961 aggatatgca agccattcga attttctatt ttaatttcct tttacaaaga aatgtataac
    10021 aaaatataat ttgaaaaaat gttctggctc taattcgatt tcttttaagt attttgtgaa
    10081 ctgcttttaa taacgagcgg ttgcaaaact taacagaaac gtgcactttg atcccaacta
    10141 ataccgtgta ctatcacgtg tttggtttta agtactcatc cttcgtgttt cgttgtttgt
    10201 tgtttgcctt gctgtacact tggctcttgc gctctctcgc tctccgttgg agccggcttt
    10261 ttgaatgcat gcctcgccct gctcgcgccc agtcctcctc catcaacttt gtatacaaaa
    10321 gttgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
    10381 aaacagacta cataatactg taaaacacaa catatccagt cactatgggc cgcattaggc
    10441 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
    10501 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
    10561 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
    10621 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
    10681 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
    10741 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
    10801 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
    10861 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
    10921 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtgtc agccaatccc
    10981 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
    11041 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
    11101 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
    11161 cagtactgcg atgagtggca gggcggggcg taaacgcgtg gcacatatac ctgccgttca
    11221 ctattattta gtgaaatgag atattatgat attttctgaa ttgtgattaa aaaggcaact
    11281 ttatgcccat gcaacagaaa ctataaaaaa tacagagaat gaaaagaaac agatagattt
    11341 tttagttctt taggcccgta gtctgcaaat ccttttatga ttttctatca aacaaaagag
    11401 gaaaatagac cagttgcaat ccaaacgaga gtctaataga atgaggtcga aaagtaaatc
    11461 gcgcgggttt gttactgata aagcaggcaa gacctaaaat gtgtaaaggg caaagtgtat
    11521 actttggcgt caccccttac atattttagg tcttttttta ttgtgcgtaa ctaacttgcc
    11581 atcttcaaac aggagggctg gaagaagcag accgctaaca cagtacataa aaaaggagac
    11641 atgaacgatg aacatcaaaa agtttgcaaa acaagcaaca gtattaacct ttactaccgc
    11701 actgctggca ggaggcgcaa ctcaagcgtt tgcgaaagaa acgaaccaaa agccatataa
    11761 ggaaacatac ggcatttccc atattacacg ccatgatatg ctgcaaatcc ctgaacagca
    11821 aaaaaatgaa aaatatcaag ttcctgaatt cgattcgtcc acaattaaaa atatctcttc
    11881 tgcaaaaggc ctggacgttt gggacagctg gccattacaa aacgctgacg gcactgtcgc
    11941 aaactatcac ggctaccaca tcgtctttgc attagccgga gatcctaaaa atgcggatga
    12001 cacatcgatt tacatgttct atcaaaaagt cggcgaaact tctattgaca gctggaaaaa
    12061 cgctggccgc gtctttaaag acagcgacaa attcgatgca aatgattcta tcctaaaaga
    12121 ccaaacacaa gaatggtcag gttcagccac atttacatct gacggaaaaa tccgtttatt
    12181 ctacactgat ttctccggta aacattacgg caaacaaaca ctgacaactg cacaagttaa
    12241 cgtatcagca tcagacagct ctttgaacat caacggtgta gaggattata aatcaatctt
    12301 tgacggtgac ggaaaaacgt atcaaaatgt acagcagttc atcgatgaag gcaactacag
    12361 ctcaggcgac aaccatacgc tgagagatcc tcactacgta gaagataaag gccacaaata
    12421 cttagtattt gaagcaaaca ctggaactga agatggctac caaggcgaag aatctttatt
    12481 taacaaagca tactatggca aaagcacatc attcttccgt caagaaagtc aaaaacttct
    12541 gcaaagcgat aaaaaacgca cggctgagtt agcaaacggc gctctcggta tgattgagct
    12601 aaacgatgat tacacactga aaaaagtgat gaaaccgctg attgcatcta acacagtaac
    12661 agatgaaatt gaacgcgcga acgtctttaa aatgaacggc aaatggtacc tgttcactga
    12721 ctcccgcgga tcaaaaatga cgattgacgg cattacgtct aacgatattt acatgcttgg
    12781 ttatgtttct aattctttaa ctggcccata caagccgctg aacaaaactg gccttgtgtt
    12841 aaaaatggat cttgatccta acgatgtaac ctttacttac tcacacttcg ctgtacctca
    12901 agcgaaagga aacaatgtcg tgattacaag ctatatgaca aacagaggat tctacgcaga
    12961 caaacaatca acgtttgcgc caagcttcct gctgaacatc aaaggcaaga aaacatctgt
    13021 tgtcaaagac agcatccttg aacaaggaca attaacagtt aacaaataaa tgtcaggctc
    13081 ccttatacac agccagtctg cacgtcgcat agtgactgga tatgttgtgt tttacagtat
    13141 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
    13201 gtttctcgtt caacttttta atacaaagtt gcctccggaa cataatggtg catctagatg
    13261 cccattcctg tagacttctt aattaagacg tcagaattct cgaggcggcc gcatgtgagt
    13321 ctccctatag tgagtcgtat taatttcgcg ggcgcgtcag cgggagagcc gttgtaaggc
    13381 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
    13441 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
    13501 tgcctgtgat caattcgggc acgaacccag tggacataag cctgttcggt tcgtaagctg
    13561 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
    13621 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttg
//
