LOCUS       sequence-405854-       10393 bp    DNA     circular SYN 14-APR-2025
DEFINITION  For generating targeting inversions. C-terminal split GFP, expressed
            in abdomen and mouthparts..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10393)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 10393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT25
FEATURES             Location/Qualifiers
     source          1..10393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             127..918
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1164..1752
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1653..1672
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1906..1923
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1955..1973
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1955..1972
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        2050..2470
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     protein_bind    2477..2600
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2623..2628,2629..2646,2647..2653)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        2696..2800
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2801..2869,2870..3661)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3019..3038)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     4182..4201
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4341..4464)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     enhancer        4516..4998
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        5002..5593
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             6718..6765
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     polyA_signal    6876..6997
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6913..6932)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6967..6986
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    7029..7152
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7175..7180,7181..7198,7199..7205)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7259..7918
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7325..7344)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        7927..8372
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(8373..8459,8460..9794)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     protein_bind    complement(9833..9956)
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(10048..10067)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10049..10067)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
       61 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
      121 aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
      181 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
      241 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
      301 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
      361 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
      421 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
      481 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
      541 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
      601 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
      661 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
      721 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
      781 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
      841 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
      901 aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc
      961 gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag
     1021 gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat
     1081 atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1141 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1201 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1261 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     1321 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1381 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1441 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1501 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1561 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1621 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1681 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1741 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1801 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1861 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1921 gaggaaatcg tcacctgcag gaaggtttaa acgcatttag gtgacactat agaagtgtgt
     1981 atcgctcgag ggatccgaat tcgaagtctt ggtacggagg ggatccgatc gacaagccga
     2041 acatatgggt tttttgctca cctgtgattg ctcctactca aatacaaaaa catcaaattt
     2101 tctgtcaata aagcatattt atttatattt attttacagg aaagaattcc ttttaaagtg
     2161 tattttaacc tataatgaaa aacgattaaa aaaaatacat aaaataattc gaaaattttt
     2221 gaatagccca ggttgataaa aattcatttc atacgtttta taacttatgc ccctaagtat
     2281 tttttgacca tagtgtttca attctacatt aattttacag agtagaatga aacgccacct
     2341 actcagccaa gaggcgaaaa ggttagctcg ccaagcagag agggcgccag tgctcactac
     2401 tttttataat tctcaacttc tttttccaga ctcagttcgt atatatagac ctattttcaa
     2461 tttaacgtcg gggcctcaac tttgtataat aaagttgaac gagaaacgta aaatgatata
     2521 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
     2581 caacatatcc agtcactatg ggccgcatta ggcaccccag gctttacact ttatgcttcc
     2641 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctacgcgg
     2701 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2761 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     2821 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     2881 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     2941 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3001 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3061 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3121 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3181 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3241 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3301 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     3361 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3421 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     3481 gtttattgct gataaatctg gagccggtga gcgtgggtcc cgcggtatca ttgcagcact
     3541 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     3601 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     3661 atggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg
     3721 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa
     3781 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat
     3841 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
     3901 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
     3961 aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta
     4021 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta tcgatacacc
     4081 gggccgtcgc atggttattc cacttgcgtc tgcgcgcttg ctctcagata aagtctcccg
     4141 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
     4201 ggccagtgtg ccggtttccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
     4261 tgacatcaaa aacgccatta acctgatgtt ctggggaata taatgtcagg ctcccttata
     4321 cacagccagt ctgcacgtcg catagtgact ggatatgttg tgttttacag tattatgtag
     4381 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     4441 gttcaacttt tctatacaaa gttgtccagc tggagcgaga ccggagacta gttgttcgcc
     4501 gagctcttac gcgcccgcgt aaaacacaat caagtatgag tcataagctg atgtcatgtt
     4561 ttgcacacgg ctcataaccg aactggcttt acgagtagaa ttctacttgt aacgcacgat
     4621 cagtggatga tgtcatttgt ttttcaaatc gagatgatgt catgttttgc acacggctca
     4681 taaactcgct ttacgagtag aattctacgt gtaacgcacg atcgattgat gagtcatttg
     4741 ttttgcaata tgatatcata caatatgact catttgtttt tcaaaaccga acttgattta
     4801 cgggtagaat tctacttgta aagcacaatc aaaaagatga tgtcatttgt ttttcaaaac
     4861 tgaactcgct ttacgagtag aattctacgt gtaaaacaca atcaagaaat gatgtcattt
     4921 gttataaaaa taaaagctga tgtcatgttt tgcacatggc tcataactaa actcgcttta
     4981 cgggtagaat tctacgcgcg tcgatgtctt tgtgatgcgc gcgacatttt tgtaggttat
     5041 tgataaaatg aacggatacg ttgcccgaca ttatcattaa atccttggcg tagaatttgt
     5101 cgggtccatt gtccgtgtgc gctagcatgc ccgtaacgga cctcgtactt ttggcttcaa
     5161 aggttttgcg cacagacaaa atgtgccaca cttgcagctc tgcatgtgtg cgcgttacca
     5221 caaatcccaa cggcgcagtg tacttgttgt atgcaaataa atctcgataa aggcgcggcg
     5281 cgcgaatgca gctgatcacg tacgctcctc gtgttccgtt caaggacggt gttatcgacc
     5341 tcagattaat gtttatcggc cgactgtttt cgtatccgct caccaaacgc gtttttgcat
     5401 taacattgta tgtcggcgga tgttctatat ctaatttgaa taaataaacg ataaccgcgt
     5461 tggttttaga gggcataata aaagaaatat tgttatcgtg ttcgccatta gggcagtata
     5521 aattgacgtt catgttggat attgtttcag ttgcaagttg acactggcgg cgacaagatc
     5581 gtgaacaacc aagtgaccat gaagatcgag gagggcaagt tggtcatctg gattaatgga
     5641 gataagggct ataatggact ggcagaggtt ggaaaaaaat ttgagaagga tacaggcatt
     5701 aaggtcacag ttgagcatcc agataagttg gaggagaagt tcccgcaggt ggctgcgacg
     5761 ggtgacggac ccgacatcat attctgggct catgaccgtt tcggaggata cgcccagtcc
     5821 ggcttgttgg cggaaattac ccctgataaa gcttttcagg acaaactcta ccccttcacc
     5881 tgggatgctg tccgatacaa cggcaagctc attgcatacc ccattgcagt tgaagcactg
     5941 agtctgattt acaataaaga cctgttgccg aatcctccga agacctggga ggagattccc
     6001 gctctggaca aggagctgaa ggctaagggc aagtccgccc tgatgttcaa cctgcaagag
     6061 ccgtacttta cttggcccct gatcgcagcc gacggcggat acgccttcaa atacgagaac
     6121 ggaaagtacg atataaagga tgtcggagtc gacaacgccg gcgctaaagc aggcctgact
     6181 ttcctggtgg acctcataaa gaacaagcat atgaatgccg acaccgatta cagtatagcg
     6241 gaagccgcgt tcaacaaggg cgagactgcc atgacaataa acggaccctg ggcatggtcc
     6301 aacattgaca cgagcaaggt gaactacggt gtaacggtgt tgcctacgtt caagggacag
     6361 ccgagtaaac cattcgttgg cgttttgtcg gctggaatta atgctgctag tcccaataag
     6421 gagttggcta aggagttctt ggagaactac ttgctgacag acgagggctt ggaggcagta
     6481 aacaaggata agcccctggg tgcggttgcc ctcaagtcct atgaagaaga gctcgtaaaa
     6541 gacccgcgta ttgctgcgac catggagaat gcccagaagg gtgagattat gcccaatata
     6601 ccccagatgt ccgcgttttg gtatgcagtt cgcaccgcgg ttataaacgc agcctcggga
     6661 cggcagaccg tggatgagga cttgaaagac gcccagacac gcataaccaa acagtttcgc
     6721 gaccatatgg ttctgcatga atatgtcaac gcagcaggca ttacttaaag atcataatca
     6781 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     6841 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     6901 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6961 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaaag cttatcgata cgcccatggt
     7021 ctcaccatca actttgtata caaaagttga acgagaaacg taaaatgata taaatatcaa
     7081 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
     7141 ccagtcacta tgggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7201 taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat
     7261 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
     7321 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
     7381 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
     7441 cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga
     7501 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
     7561 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
     7621 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
     7681 tatgtttttc gtgtcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
     7741 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
     7801 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt
     7861 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaac
     7921 gcgtggcaca tatacctgcc gttcactatt atttagtgaa atgagatatt atgatatttt
     7981 ctgaattgtg attaaaaagg caactttatg cccatgcaac agaaactata aaaaatacag
     8041 agaatgaaaa gaaacagata gattttttag ttctttaggc ccgtagtctg caaatccttt
     8101 tatgattttc tatcaaacaa aagaggaaaa tagaccagtt gcaatccaaa cgagagtcta
     8161 atagaatgag gtcgaaaagt aaatcgcgcg ggtttgttac tgataaagca ggcaagacct
     8221 aaaatgtgta aagggcaaag tgtatacttt ggcgtcaccc cttacatatt ttaggtcttt
     8281 ttttattgtg cgtaactaac ttgccatctt caaacaggag ggctggaaga agcagaccgc
     8341 taacacagta cataaaaaag gagacatgaa cgatgaacat caaaaagttt gcaaaacaag
     8401 caacagtatt aacctttact accgcactgc tggcaggagg cgcaactcaa gcgtttgcga
     8461 aagaaacgaa ccaaaagcca tataaggaaa catacggcat ttcccatatt acacgccatg
     8521 atatgctgca aatccctgaa cagcaaaaaa atgaaaaata tcaagttcct gaattcgatt
     8581 cgtccacaat taaaaatatc tcttctgcaa aaggcctgga cgtttgggac agctggccat
     8641 tacaaaacgc tgacggcact gtcgcaaact atcacggcta ccacatcgtc tttgcattag
     8701 ccggagatcc taaaaatgcg gatgacacat cgatttacat gttctatcaa aaagtcggcg
     8761 aaacttctat tgacagctgg aaaaacgctg gccgcgtctt taaagacagc gacaaattcg
     8821 atgcaaatga ttctatccta aaagaccaaa cacaagaatg gtcaggttca gccacattta
     8881 catctgacgg aaaaatccgt ttattctaca ctgatttctc cggtaaacat tacggcaaac
     8941 aaacactgac aactgcacaa gttaacgtat cagcatcaga cagctctttg aacatcaacg
     9001 gtgtagagga ttataaatca atctttgacg gtgacggaaa aacgtatcaa aatgtacagc
     9061 agttcatcga tgaaggcaac tacagctcag gcgacaacca tacgctgaga gatcctcact
     9121 acgtagaaga taaaggccac aaatacttag tatttgaagc aaacactgga actgaagatg
     9181 gctaccaagg cgaagaatct ttatttaaca aagcatacta tggcaaaagc acatcattct
     9241 tccgtcaaga aagtcaaaaa cttctgcaaa gcgataaaaa acgcacggct gagttagcaa
     9301 acggcgctct cggtatgatt gagctaaacg atgattacac actgaaaaaa gtgatgaaac
     9361 cgctgattgc atctaacaca gtaacagatg aaattgaacg cgcgaacgtc tttaaaatga
     9421 acggcaaatg gtacctgttc actgactccc gcggatcaaa aatgacgatt gacggcatta
     9481 cgtctaacga tatttacatg cttggttatg tttctaattc tttaactggc ccatacaagc
     9541 cgctgaacaa aactggcctt gtgttaaaaa tggatcttga tcctaacgat gtaaccttta
     9601 cttactcaca cttcgctgta cctcaagcga aaggaaacaa tgtcgtgatt acaagctata
     9661 tgacaaacag aggattctac gcagacaaac aatcaacgtt tgcgccaagc ttcctgctga
     9721 acatcaaagg caagaaaaca tctgttgtca aagacagcat ccttgaacaa ggacaattaa
     9781 cagttaacaa ataaatgtca ggctccctta tacacagcca gtctgcacgt cgcatagtga
     9841 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa
     9901 tatattgata tttatatcat tttacgtttc tcgttcaact ttttaataca aagttgcctc
     9961 cggaacataa tggtgcatct agatgcccat tcctgtagac ttcttaatta agacgtcaga
    10021 attctcgagg cggccgcatg tgagtctccc tatagtgagt cgtattaatt tcgcgggcgc
    10081 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
    10141 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
    10201 gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac
    10261 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
    10321 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
    10381 tgactgtttt ttt
//
