LOCUS       sequence-405977-        3312 bp    DNA     circular SYN 15-APR-2025
DEFINITION  3' downstream region of pyrG gene in P. digitatum..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB361
FEATURES             Location/Qualifiers
     source          1..3312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    682..703
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      706..749
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(753..771)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             933..1592
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(999..1018)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1613..1707
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1890..2478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2379..2398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2632..2649
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2673..2730)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 ttacttggat agaatcgagg ccgctagaac gaacgggcac attgattgtg ccagtccgtt
       61 tttgatgggc tatctgaatg ggaatggttg cacgtgatgg gattagcgtg tcgcatttta
      121 ttgatgtagc ttactcagcc cgctcatccc tacactcagt gagcactcag tgatcaaatc
      181 agagtgctca gatgatgctc agtgagcaga tcagttgatt atgtaatgac ctgtgatcaa
      241 gccaggatac tcagggacgt gctcggtttt actcagtgat caaaaagcgg caaggggtga
      301 gcactaaaac ggaaagggaa agatctcgag tgccaggcat tatccggtct atatgtcgac
      361 atgcttacgc ttctagttct gctggttctg atgaggttga cacataatta cagtattact
      421 ctgatctgtt gtccggtatc tcgacatctg aagtttggag ccgccttatc aagctactcc
      481 caacattcga atattcagag attcgcgttt gatttgatga ttcaatgaag ggggagtgaa
      541 ccatatcgtc gagcacacgt aagacttgaa attgcccttg catcgtttac acatgcaggg
      601 aagccctttt agagccctgg aaagactcac taataatctg aggcaagaaa gtaccgggct
      661 ggcattcgag accactcatc gctctagaag cggccgcgaa ttccagaaat catccttagc
      721 gaaagctaag gatttttttt atctgaaatt ctgcctcgtg atacgcctat ttttataggt
      781 taatgtcatg ataataatgg tttcttagac gtcaggtggc actcgagttg atcgggcacg
      841 taagaggttc caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt
      901 tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
      961 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
     1021 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
     1081 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
     1141 atccggaatt tcgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
     1201 cttgttacac cgttttccat gagcaaactg aaacgttttc atcactctgg agtgaatacc
     1261 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
     1321 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtatca gccaatccct
     1381 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
     1441 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
     1501 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
     1561 agtactgcga cgagtggcag ggcggggcgt aatttgatat cgagctcgct tggactcctg
     1621 ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc
     1681 cgccgggcgt tttttattgg tgagaatcca agcctcgagc tgtcagacca agtttactca
     1741 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1801 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1861 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1921 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1981 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     2041 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2101 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2161 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2221 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2281 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2341 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2401 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2461 gggcggagcc tgtggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2521 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2581 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2641 gtgagcgagg aagcctgcat aacgcgaagt aatcttttcg gttttaaaga aaaagggcag
     2701 ggtggtgaca ccttgccctt ttttgccgga ctgcagcggc cgctactagt cgcgatgagt
     2761 ggtctcaaag caggcagagg aatgatgaag accattgttt ccgagcaagt ggctagatac
     2821 atatcccttt tggaatggca tcgctttagg cagaaatcag acaggctagg ggtggggggc
     2881 aatgtttaga gttcctagga ttcaaacgaa atagctatgg gcccctatga cactggtaat
     2941 acagcttagt gggctcatgg gaatgcatgg caggctccca ggagctcatc gggtattatt
     3001 attgatggtt tgtcttcggc ttacagatta cttagcaatg gcgtgaaaga catgacgtta
     3061 tctgcccagg agatcctttg ggtcgtgcgc tcattggcga cagaagtggc tcatcatcgt
     3121 cacttactga acactaattt ctcctgttgc cgacacaggg aatgattcta gatcttcaag
     3181 gaaactcatt caatcgccct cgccctggta gtagcccaaa gcccgattag ggtattccgt
     3241 gaggtattcc caggagcata ggctgaaatg agaagatggt aggtcaatta atagccccac
     3301 tgtggggctt tt
//
