LOCUS       sequence-405979-        3313 bp    DNA     circular SYN 15-APR-2025
DEFINITION  5' upstream region of pyrG gene in P. digitatum..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB359
FEATURES             Location/Qualifiers
     source          1..3313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(443..537)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(558..1217)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1132..1151
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1379..1397
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1401..1444)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1447..1468
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2733..2790
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2814..2831)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2985..3313,1..260))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3065..3084)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
       61 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
      121 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
      181 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
      241 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
      301 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
      361 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
      421 ggtctgacag ctcgaggctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg
      481 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
      541 gcgagctcga tatcaaatta cgccccgccc tgccactcgt cgcagtactg ttgtaattca
      601 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc
      661 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag
      721 aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca gggattggct
      781 gatacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa
      841 cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc
      901 cagagtgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta
      961 tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc
     1021 aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc
     1081 tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac
     1141 tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca
     1201 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat
     1261 acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc
     1321 aactcgagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     1381 taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga
     1441 tttctggaat tcgcggccgc ttctagagcg atgagtggtc tcgggaggat gaaggatcca
     1501 gaaaatcctt caatctgaag tcatcctcaa tgctccgaaa ggccaactat atcacaacag
     1561 tagacaagaa aaccttggaa gaccccggat cggctaaatc gattgcaccg acgggacgtc
     1621 ggaacctgtg agccccgaac ctccaaatat gtataaagga gcccaggcat caggctaaat
     1681 cacaacacaa agccgctctc tcacttcatt tccccggtta atcttctcat aatggtcgca
     1741 ttcattgttc ctggcaacta cgggtaggtt atgccactcc cggcttaacc ttcgcgtttc
     1801 ctgtgaaatc cggggctaac ataacttccc agagccgtca ttgccgtggc cctcggtgcc
     1861 atccctgttt tgggctttat tcacggcaat gtcactggta gcctgcgcaa ggcggccaag
     1921 gtgccctacc cccacagcta tgcctccatg gagctatgca aggaaaacgt gcgtagaaat
     1981 tttttttcca tctgtcggcc ttcaaaagcc cttcagttca agggaaacat gtgtaatctc
     2041 taatgaacac cccattaaac ctgctaatct gattataggc aaaagctgag cagttcaact
     2101 gcgcccagcg cgcccatgcc aacttcctcg agaacgcctc gcagacaatg ctctttacct
     2161 tggtcgccgg tctaaagtat ccggagtggg ccgctggtct gggcgctctc tgggttttct
     2221 tccgcgttct cttcctctac ggctacgttt actcgggcaa gccgcagggt aagggccgct
     2281 tcatgggcgg gttcttctgg cttgtgcagg gtgctctttg gggattgagc atctacggtg
     2341 ttggaaggcc tctgctttct ttctaagagc ttgaagcctg gggatcctct aaaatgtctt
     2401 gaaattgaag atgtactctg tatttataag tccaatgcaa gcaacgtatt agttatgcgc
     2461 cacagttcca gcctcggaag gttatgttgt aggcctgcgg ggcccaccgt ggcagaaaaa
     2521 gttccccgat ggtcttcaaa tcgaacttgc tcctcatctt ctgctttttg gaccccaaaa
     2581 agcctatagt tctctgaagg catttgtcgt ttgattcatt tccatccttt cgacccctca
     2641 aaacacaagc taaatacaca acaatcgcct cttcaccccg ccagccccgc caatgtgaga
     2701 ccactcatcg cgactagtag cggccgctgc agtccggcaa aaaagggcaa ggtgtcacca
     2761 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc
     2821 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2881 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2941 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc
     3001 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3061 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3121 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3181 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3241 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3301 aacccggtaa gac
//
