LOCUS       sequence-405994-        2872 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Promoter of Isopenicillin N synthase from P. rubens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB411
FEATURES             Location/Qualifiers
     source          1..2872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      809..866
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(890..907)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1061..1649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1141..1160)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1832..1926)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1947..2606)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2521..2540
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2768..2786
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2790..2833)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2836..2857
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggagggtcga ctacatgtat ctgcatgttg catcgggaaa tcccaccaca gggacagcca
       61 agcggccccg cgacttggca gtgggcaaac tacgcccgat tctggtgcca agaaccgaga
      121 agaatgagac agacccacgt tgcactctaa ccggatgcta tcgacttacg gtggctgaag
      181 attcaacacg ctgcaacgag agccaaggtg gtccggacat tttctacgtg ccggtttacc
      241 ttggaacatc gccgtcgttg agtgcacgtt gcctactctc tcgtggcttg gctgggccca
      301 cgagcccgat tgactcgacg gtgttacttg ggtatctatg gccccgtttt ctggcacggt
      361 aatgataagt acttactagt cttcgagcga gcgggggagt gttgctctgc ccgagcatca
      421 acgattggcc tgatcgcacc gtctgcaaat gccacggtgc ggaccgactg aaatctcaga
      481 ccaccaaaga ccctccgact tcgagatacg gttactaatt ttacactggc tccagcggcc
      541 ccatccagta agcatctggg ctgcaagcgt ataatgtctc caggttgtct cagcataaac
      601 accccgcccc cgctcaggca cacaggaaga gagctcaggt cgtttccatt gcgtccatac
      661 tcttcactca ttgtcatctg caggagaact tcccctgtcc ctttgccaag ccctctcttc
      721 gtcgttgtcc acgccttcaa gttttcacca ttatttttct agacaccaat gtgagaccac
      781 tcatcgcgac tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct
      841 gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg
      901 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      961 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     1021 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc
     1081 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1141 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1201 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1261 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1321 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1381 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1441 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1501 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1561 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1621 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1681 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     1741 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1801 agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc
     1861 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     1921 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcgtc gcagtactgt
     1981 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     2041 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     2101 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     2161 ggattggctg atacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     2221 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     2281 tattcactcc agagtgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     2341 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
     2401 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     2461 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     2521 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     2581 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     2641 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     2701 tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     2761 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
     2821 taaggatgat ttctggaatt cgcggccgct tctagagcga tgagtggtct cg
//
