LOCUS       sequence-406585-       13176 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Express mEGFP-tagged fusion protein, BCR_ABL1_p210 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13176)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 13176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_BCR_ABL1
FEATURES             Location/Qualifiers
     source          1..13176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(7937..8647,8648..8650,8651..8653))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7979..8000)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8328..8347
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8587..8608
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8650..8659
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8735..8938)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8764..8784)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8939..9242
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(9499..9543)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9728..9961
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10321..10438
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    10930..11055
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             11102..11359
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        11361..11740
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11771..11875
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11876..11944,11945..12736)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12094..12113)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(12907..13176,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggtctacc
     1801 tctgcactat gtcactgatt tccttcaccg aactgaggag cttgctgaag tcctgagtgg
     1861 ccgctggacc actgcctgct gtcgccgggc agatctgaag ctcccggaga ttattctcca
     1921 gtttgttgat ggcctctcgg aaggcaaact tgttcctcat ttgctggatg gaatccacat
     1981 agctcacgca gaacgagtag aggtttttgc cggcctccag cactgcgctg tggctggcca
     2041 tctgctcgga gttcctagag atggcgaggc acagcgcctc ggtgctgtcc aggaccacgc
     2101 ccttggtgat ggcgccgctg gcgatccgct ctggaggctg gcgggttttc cgaagagaca
     2161 ctcgggttga tatgagaggg atgaaggcgg tggaagacgg ctggtcccct gccagggccg
     2221 aggatgctga tggcaacgtg gagggaacgg gggctgggct gatgggggtc cccgacggct
     2281 tggcggactg tggctttgga gtggccggga gcacgggctt tttgaggccc tctcccggct
     2341 ggctgggctt ggcagcgtca ctgttcacag catcaaccag actcgtggct tttgtctttg
     2401 cgcccaggac tgcctccccg gccgcctcct ggctcgggct ctgcgagggc tttcctccag
     2461 ccttccctgc agaggctgct ggtgggggcg gcggggcagg tttgagcctg gacaatttcc
     2521 ccttgtccct ccctggcgac tcagaggagt gcttgtgcct cctcactctg gactcctcgg
     2581 cggggccctt gctggtgccc cctggtgcac ctgagcctgc tttgctggtg ggggtcactg
     2641 gctcagctgc agcaggggtc cctaaggcac tgccctttcc agcttcttcc ttgtggggga
     2701 ggcccgaggc aggggccacg gtgacctgcc gccggagggg ttttggagtc aggttgggcg
     2761 ggctggagcc cgggctggac tccatgatgt ctttgaagac ctcatcagca gcttcctcat
     2821 tctttttcac cagcctgggg ggaggcgtta ctgtgcctcg ggtcacctgg tcagacctgt
     2881 tctcccctgc cctcttccga ggcagagccg gcttctcact tttgtgccct ccaaatgtgg
     2941 acgagtcaaa ctgtcttccc gtggactgca agtcccgagg cagcgtgact gacctccact
     3001 ccgtgtcctt ggccccatgg ggaacgcagg aggcggagca agagcgcagg aagcgcttgc
     3061 tggagctgcc accgccctcc tcctcgccgg tggctaggcg gctgctggtc agcgtgctgg
     3121 acttcttcca caggtgggga gaccggaagc ctgagccccc ggactcccgg agggctccat
     3181 tggggacccc agccccattg ctgggctttg gggacttggc tgggtcagct gtgtccaagg
     3241 gggtgaaagc cagtgccccg ttgctgatgt ctcggccctc ttcctcgccg gcccctctgc
     3301 gctccggctg gccgtccatc tcccggaagg agctgctgcg tttgggaggg gttggggctg
     3361 tcttcttctt cttcttgatc aaggcgctga acaagttggt ctttttgtct ttggggagaa
     3421 ggcgctcatc ttcattcagg ccgccctccg ggggacctcg ctcttttcga gggagcaatg
     3481 gagacacggc aggctcatgg tccagaggat cgctctctcc ctggcccttg gagtgaggca
     3541 tctcaggcac gtcagtggtg tctctgtgct ctgcagctct cctggaggtc ctcgtcttgg
     3601 tgggcagctc tggggcctgc agcaaggtac tcacagcccc acggacgcct tgtttcccca
     3661 gctccttttc cacttcgtct gagatactgg attcctggaa cattgtttca aaggcttggt
     3721 ggatttcagc aaaggagggc cggtcagagg gattccactg ccaacatgct cgcatgagtt
     3781 catagacctt ctctgggcag ccttctgggc gctccatgcg gtagtccttc tctagcagct
     3841 catacacctg ggacaggtca attcccgggt aaggggacat gccataggta gcaatttccc
     3901 aaagcaatac tccaaatgcc cagacgtcgg acttgatgga gaacttgttg taggccaggc
     3961 tctcgggtgc agtccatttg atggggaact tggctccagc atgggctgtg taggtgtccc
     4021 ctgtcatcaa cctgctcagg ccaaaatcag ctaccttcac caagtggttc tcccctacca
     4081 ggcagtttcg ggcagcaaga tctctgtgga tgaagttttt cttctccagg tactccatgg
     4141 ctgacgagat ctgagtggcc atgtacagca gcaccacggc gttcacctcc tgccggttgc
     4201 actccctcag gtagtccagg aggttcccgt aggtcatgaa ctcagtgatg atatagaacg
     4261 ggggctcccg ggtgcagacc ccaaggagct gcaccaggtt agggtgtttg atctctttca
     4321 tgactgcagc ttctttcaag aactcttcca cctccatggt gtcctccttc aaggtcttca
     4381 cggccaccgt caggctgtat ttcttccaca cgccctcgta cacctccccg tactggcccc
     4441 cgcccagctt gtgcttcatg gtgatgtccg tgcgttccat ctcccacttg tcgtagttgg
     4501 gggacacacc atagacagtg ggcttgttgc gctttggggc tggataatgg agcgtggtga
     4561 tgagcccgtc ggccaccgtt gaatgatgat gaaccaactc ggccagggtg ttgaagcggc
     4621 tctcggagga gacgtagagc ttgccatcag aagcagtgtt gatcctgtaa tggtacaccc
     4681 tcccttcgta tctcagcgag atggacctct ggccaggact gctctcactc tcacgcacca
     4741 agaagctgcc attgatcccg ctgctcagca gatactcagc ggcattgcgg gacacaggcc
     4801 catggtacca ggagtgtttc tccagactgt tgactggcgt gatgtagttg cttgggaccc
     4861 agccttggcc atttttggtt tgggcttcac accattcccc attgtgatta tagcctaaga
     4921 cccggagctt ttcaccttta gttatgctta gagtgttatc tccactggcc acaaaatcat
     4981 acagtgcaac gaaaaggttg gggtcatttt cactgggtcc agcgagaagg ttttccttgg
     5041 agttccaacg agcggcttca ctcagaccct gaggctcaaa gtcagatgct actggccgct
     5101 gaagggcttt tgaactctgc ttaaatccag tggctgagtg gacgatgaca ttcagaaacc
     5161 catagagccc cggagactca tcatcttcct tattgatggt cagcggaatg ctgtggacag
     5221 tctggagttt cacacacgag ttggtcagca tctgcagctc cacggatgtc agggagaagc
     5281 ttctgaaaca cttcttctgc tgctcccgga tgttctccct ccactctgca cgctcatagt
     5341 cagaggagat caggaacgtg taactcttgc cgttgcggct gtgcaccctg aaggccatgc
     5401 tgggagacat aagcagcagc agtgactcct gctccgacag cttcttcttc agcctctccg
     5461 tagccttgct gcccttgttc gccctcttct ctctctggat gtcactcttg atctgggaga
     5521 tcttgatctt caaagcgtcc agctcctcat cgggcaccag ggggatgttg ggcactgcct
     5581 ccagttcatc caccatctgg aagctgagat ccgtgagcgg aatgtaccat ttgcagtcat
     5641 actgctgcgt tttgcctccg ctctgcttct tgagcttggt gcagagaagc aggtcggtga
     5701 acaggaagac gtggcgcagc ttgcgggccc cctccaccag ctccaccatg aagctgtcct
     5761 tcagcagctg ccggtgctct cccttcttca ccgtcatgga ctgccgtcgg ggtgtgatct
     5821 cctcattgat gctggacagg aagttctgtg agatgcggag ggcgtcctgc agcaaggggt
     5881 ggtcagggtg gctggcagga gtgtgcttca gcaagtcatg gaggaccagc gtgctcctcg
     5941 tcacacggtc cacaggcttg tagagcagag tttccagaga gttcttggtc gttggatcct
     6001 tggcatcttt gttgcttctg gctctcaggt tctcggagat ttctgcaaac tgagcattgg
     6061 cctgacagca cttctcagcc atttccatgg caactccgta gttgtccacg aaggcccggt
     6121 acacacccag ctggctggcc agcttctgga agaggtcgcc cacccgctgc tggtggctcc
     6181 actgctgcac gcgggggaag agcccatcat agaactcctt gtggatctcg tagagctcag
     6241 gcactttgaa gaagatggtc tcgatctgct gactcgtcag caccggctga gaggtggtgg
     6301 cagcggcttt caaaggcttc atgggcagca gcagtgcctc caggtggctc aggtaagtct
     6361 cctcgctagc caggattccc gacaggaccc attttctcat ctccaagccc ttttccaagt
     6421 ccagctcact cgctttagtg gactccaggg ctcccgagac cagcgcatcc cggctgcccc
     6481 tgcccttgct gctgaggtgg ggcgatgagg agggcgagtc atcaatgtag ggcagcccat
     6541 cttggtgccg gcgctgctcc tctgcccggt ctgcagcgca gccgtatcca ggtggtgttc
     6601 cgaacgagcc atctgcgtct ccatggaagg cgccctcgcc atcgttgggc cagatctgcc
     6661 cggtcttgcg gacgcccacg atggtggcct cggacacgac aaccgggcag tgccggtgcc
     6721 gcttatggca ctgcggcgtg gggggactgc tgctgtcgaa ggactgttgc gagttctgcg
     6781 agggagagcg gcttttgtcc cggaacatgc ggtaggtggt ggggcttggg gacacgcggc
     6841 tggactggcc agaggagaag tcctcctcgc tggaggtgag gttctcattg gagctgcagt
     6901 ccggggtata gccgcctccg caatcctcaa aactccgggg ggagtaggac ctgcggggcc
     6961 aggtaaggcg cttctcctgc tcagaggtgc tctggctgcg caggagcggg cccttgccct
     7021 ccccttccat catgcccccg acgtagatgc tctggtaggg ctggtactcc aggggcggcc
     7081 aagggggcct gctaccgcca ttggcgtcga tcaggttgtc cttcaggaag cgggggttca
     7141 actcggcgtc ctcgtagtcg ccgtcgacgc cgcagctgct ctccgaggag cgtccccggt
     7201 aagggggcct ggatgcatcc cccacgctcg agcccgcgcc gtgctgggac tttttgcgct
     7261 ccatctgcat ggcctggctg cccagggagc tgatgcggtc cgacacctct ttgtcgttga
     7321 ccttcaccag gccgcgctcg tggtgaaact cgacgttcac gtagaagggc ttctcggcgt
     7381 ccgccccggg ctggccatgg cccttgcgga tccgctcgaa gttggacctg agcgccgcca
     7441 cgctggcggg gggtccccgg tcgtcccgtt cccccgacgc ggctgccccg ggcctgcggg
     7501 cggtcccggg cctggcctta cccggagaac cctcgccgtc gggccgggcc tcgggctcct
     7561 cggcgggcgg cgggtcggct ccgtcggcgg gcgctggctg cgggcgcgac gcggacgctc
     7621 ggggctcgga ggcgccgtcg ggggcctgcg ccgcgcgccg gaagccccat cgctgccggt
     7681 catagctctt cttttccttg gccagcaacg tctgcaggta gatcatgcgg aagcgctcct
     7741 ggttcacctc ctgctccagg cgccgaatgg aggccttgca gcgctccagc tcctgctcga
     7801 tgtcgcccac tgagcgcagc tccatgcgcg ggggctctga gtccgggaac tgcgccttcc
     7861 acgcctccgc gaagcccacc gggtccacca ttccccggga agcggccgca cttcccccgg
     7921 agcctccgct tccgcccttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca
     7981 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agcttggact
     8041 gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct
     8101 gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt
     8161 tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt
     8221 tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct
     8281 cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc
     8341 cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga
     8401 agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccgtaggtca
     8461 gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca
     8521 gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc
     8581 cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc
     8641 ccttgctcac catggtggcg accggtggat cccgggcccg cggtaccgtc gactgcagaa
     8701 ttcggttcac taaacgagcc cggttcacta aacgagctct gcttatatag acctcccacc
     8761 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
     8821 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
     8881 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca
     8941 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
     9001 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     9061 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
     9121 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
     9181 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
     9241 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
     9301 tagcacgcgt tcgaagcttg agctcggtcc cctcggggtt gggaggtggg tctgaaacga
     9361 taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct
     9421 taaacctacc aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt
     9481 tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt
     9541 cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt
     9601 aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt
     9661 actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca
     9721 aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag
     9781 ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag
     9841 caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta ccgtcagcgt
     9901 cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga acaaagctcc
     9961 tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg tgggtgctac
    10021 tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc aattgtccct
    10081 catatctcct ccttatggtg ttttactaaa cttttccatg ttctaatcct catcctgtct
    10141 acttgccaca caatcatcac ctgccatctg ttttccataa tccctaatga tctttgcttt
    10201 tcttcttggc actactttta tgtcactatt atcttgtatt actactgccc cttcaccttt
    10261 ccagaggagc tttgctggtc ctttccaaag tggatttctg ctgtccctgt aataaacccg
    10321 aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat
    10381 tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt
    10441 gtggatgaat actgccattt gtactgctgt cttaagatgt tcagcctgat ctcttacctg
    10501 tcctataatt ttctttaatt ctttattcat agattctact actccttgac tttggggatt
    10561 gtagggaatt tccaggtctg aagatcctgc tgcttgctgt gcttttttct tacttttgtt
    10621 ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta tcctttgatg
    10681 cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg
    10741 aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc
    10801 agtattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata
    10861 ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg
    10921 cttaatactg acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt
    10981 tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt
    11041 cctgcgtcga gagagctcct ctggtttccc tttcgctttc aagtccctgt tcgggcgcca
    11101 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
    11161 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc
    11221 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
    11281 ccagtacagg caaaaagcag ctgcttatat gcaggatctg agggctcgcc actccccagt
    11341 cccgcccagg ccacgcctcc catggtaata gcgatgacta atacgtagat gtactgccaa
    11401 gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca
    11461 ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca
    11521 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg
    11581 ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg
    11641 ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc
    11701 gtaattgatt actattaata actagtcaat aatcaatgtc gacccaggtg gcacttttcg
    11761 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    11821 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
    11881 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    11941 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    12001 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    12061 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
    12121 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    12181 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    12241 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    12301 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    12361 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    12421 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    12481 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    12541 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    12601 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    12661 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    12721 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    12781 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    12841 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    12901 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    12961 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    13021 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
    13081 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    13141 ggctgctgcc agtggcgata agtcgtgtct taccgg
//
