LOCUS       sequence-406768-       12639 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding
            Site motif in domain II and a mutation (W391A) in the ?1-interacting
            domain (AID) in the I-II loop that prevents Beta binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12639)
  AUTHORS   Cassidy JS, Ferron L, Kadurin I, Pratt WS, Dolphin AC
  TITLE     Functional exofacially tagged N-type calcium channels elucidate the 
            interaction with auxiliary alpha2delta-1 subunits.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8979-84. doi: 
            10.1073/pnas.1403731111. Epub 2014 Jun 2.
  PUBMED    24889613
REFERENCE   2  (bases 1 to 12639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1403731111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-06-17- 17"; volume: "111"; issue: "24"; pages: "8979-84"
COMMENT     Sequence Label: Cav2.2 BBS W391A pcDNA3
FEATURES             Location/Qualifiers
     source          1..12639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(799..817)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(800..817)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(837..854)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    843..1067
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1113..1541
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1200..1219)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1410..1431
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1550..1570)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1555..1884
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1735..1870
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1797..1816
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1951..2745
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2005..2024)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2615..2634
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2919..3040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2956..2975)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3010..3029
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3089..3105)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3089..3105)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3102..3124)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3113..3129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3137..3143,3144..3161,3162..3167))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3182..3203
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3320..3337)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3491..4079)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3571..3590)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4250..5041,5042..5110))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4873..4892
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5111..5215)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5290..5309)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5481..5860
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5861..6064
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6015..6035
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6109..6128
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6109..6127
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcgggagcg caggcaggag cggggccggt cccaggagcg gaggcagccc tcgtcctcct
       61 cctcggagaa gcatcgcttc tactcctgtg accgcttcgg gggccgcgaa cccccacagc
      121 ccaagccctc cctcagtagt caccccacgt cgccgacggc cgggcaggag ccgggacccc
      181 acccgcaggg gagtggttcg gttcacggga gccccctgct gtccacatct ggtgctagca
      241 ccccgggccg ggggcggagg cagctccccc agaccccctt gaccccccgc cccagcgtca
      301 cctacaagac ggccaactcc tcgcctgtcc acttcgccgg ggcaccgagc ggcctccccg
      361 ccttctcccc cggccggctc agccggggcc tctcagaaca caacgccctg ctgcagaggg
      421 acccgctcag ccggcccctg gcgcccggct cgcgcatcgg ctccgacccc tacctggggc
      481 agcggctgga cagcgaggcg cccgcccgtg ccctgcccga ggacgcgccc gcctttgagg
      541 agaccgcggc cagcaactcg ggccgctcct cccggacctc gtacgtgtcc tcactgacct
      601 cgcagccccc gccgctgcgc cgcgtgccca acggctacca ctgcactctg gggctgggcg
      661 ggggcggccg ggcgcggcgc ggctgccacc accccgaccg cgaccgccgg tgctagacta
      721 gttctagcaa gcttcgacga attctgcaga tatccatcac actggcggcc gctcgagcat
      781 gcatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc
      841 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
      901 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
      961 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     1021 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     1081 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     1141 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     1201 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     1261 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     1321 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     1381 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     1441 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     1501 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     1561 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     1621 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     1681 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     1741 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     1801 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     1861 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     1921 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     1981 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     2041 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     2101 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     2161 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     2221 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     2281 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     2341 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     2401 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     2461 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     2521 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     2581 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     2641 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     2701 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     2761 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     2821 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     2881 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     2941 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     3001 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     3061 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     3121 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     3181 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     3241 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     3301 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3361 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3421 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3481 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3541 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3601 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3661 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3721 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3781 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3841 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3901 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3961 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4021 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4081 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4141 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4201 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4261 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4321 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4381 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4441 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4501 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4561 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4621 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4681 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4741 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4801 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4861 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4921 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4981 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5041 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5101 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5161 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5221 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     5281 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     5341 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     5401 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     5461 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
     5521 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     5581 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     5641 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     5701 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     5761 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     5821 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     5881 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     5941 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     6001 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     6061 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
     6121 ctatagggag acccaagctt ggtaccgagc tcggatccac tagtaacggc cgccagtgtg
     6181 ctggaattcc tcgagggtac cgggcccccc ctcgaggtcg aagcttgatc atggtccgct
     6241 tcggggacga gctgggcggc cgctatgggg gcgcgggcgg cgcggagcgg gcccggggcg
     6301 gcggggccgg cggggctggc ggcccgggcc ccggggggct gccgccgggc cagcgggtcc
     6361 tctacaaaca gtcgatcgcg cagcgcgcgc ggaccatggc gctgtacaac cccatcccgg
     6421 tcaagcagaa ctgcttcacc gtcaaccgct cgctcttcgt cttcagcgag gacaacgtcg
     6481 tccgcaaata cgccaagcgc atcaccgagt ggcctccgtt cgagtacatg atcctggcca
     6541 ccatcatcgc caactgcatc gtgctggccc tggagcagca cctccctgac ggggacaaga
     6601 cgcctatgtc cgagcgcctg gatgacaccg agccctactt cattgggatc ttctgcttcg
     6661 aggcggggat caagatcctg gccctgggct ttgttctgca caagggctcc tatctgcgga
     6721 acggctggaa cgtcatggac ttcgtggtag tcctcacggg gattcttgcc acagccggaa
     6781 ccgacttcga cctgcgaacg ctgagggctg tgcgtgtgct aaggcccctg aagctggtgt
     6841 ctgggattcc gagcttgcag gtggtgctca agtccatcat gaaggccatg gtcccgctgc
     6901 tgcagatcgg gctgctgctc ttcttcgcca tcctcatgtt tgccatcatc ggcctcgagt
     6961 tctacatggg caagttccac aaggcctgct tccccaacag cacagaccca gatcccgtgg
     7021 gtgacttccc ctgtggcaag gaggccccgg cccggctctg tgagggagac acggagtgcc
     7081 gggagtactg ggccggaccc aactttggta tcaccaactt tgacaacatc ctgtttgcca
     7141 tcttgaccgt gttccagtgc atcaccatgg agggctggac tgacatcctc tacaatacaa
     7201 acgatgcagc cggcaacacg tggaactggc tctacttcat ccctctcatc atcatcggct
     7261 ccttcttcat gctcaacctg gtgctgggtg tgctctcagg agagtttgct aaggagcgcg
     7321 agagagtgga gaaccgccgc gccttcctga agctccggcg gcagcagcag attgagcggg
     7381 agctcaacgg gtacctggag gcgatcttca aggctgagga agtcatgctg gctgaggagg
     7441 acaggaacgc agaggagaag tcccctctgg atgcagtgtt gaagagagca gctgccaaga
     7501 agagcagaag cgacctgatc caggccgagg agggggaggg ccggctcaca ggcctctgtg
     7561 ctcccggttc cccgttcgcc cgtgccagcc tgaaaagcgg gaagacggag agctcgtcgt
     7621 acttccggag gaaggagaag atgttccgct tcttcatccg gcgcatggtg aaggcacaga
     7681 gcttctactg gacggtgctg tgtgtggtgg cactgaacac actgtgcgtg gccatggtgc
     7741 actacaacca gccgcagcgg ctcaccacgg cattgtactt tgcagagttt gttttcctgg
     7801 gtctcttcct cacagagatg tccctgaaga tgtatggcct ggggcccagg agctacttcc
     7861 ggtcctcctt caactgtttt gactttgggg tcatcgtggg cagtatcttt gaagtggtct
     7921 gggctgctgg ttggagatac tacgagagct ccctggagcc ctaccctgac ggcgtcgacg
     7981 gatggagata ctacgagagc tccctggagc cctaccctga cagatctgtc gacgtcaagc
     8041 cagggacctc cttcgggatc agtgttctgc gggctctgcg cttactgaga atcttcaagg
     8101 tcaccaaata ctggaactcc ctacggaacc tggtggtgtc tctgctcaac tccatgaagt
     8161 ccatcatcag cctcctcttc ctgctcttcc tgttcattgt tgtctttgct ctgctgggga
     8221 tgcagctgtt tggaggccag ttcaacttta aagacgagac cccaacgacc aactttgaca
     8281 ccttccctgc tgctatcctc accgtcttcc agatcctgac cggagaggac tggaatgcgg
     8341 tcatgtacca cgggatcgag tcgcaaggtg gcgtcagcag aggaatgttc tcctccttct
     8401 acttcattgt cctcactctg tttgggaact acaccctgct gaatgtcttc ctggccattg
     8461 ctgtggacaa ccttgccaat gctcaggagc tgaccaagga tgaagaggag atggaagaag
     8521 cagccaatca gaaactcgcc ttgcagaagg ccaaggaagt ggctgaagtc agccccatgt
     8581 ctgctgcgaa catctccatt gccgccaggc agcagaactc ggccaaagcg cgctcggtgt
     8641 gggagcagcg agccagccag ctgcggctgc agaacctgag ggccagctgc gaggcgctgt
     8701 acagcgagat ggaccccgag gagaggctgc gctacgccac cgcgcgccac ctgcggcccg
     8761 acgtgaagac gcacctggac cggccgctgg tggttgagcc cggccgcgac gctccgcggg
     8821 gccccccggg ggggaagtcc cggccggatg gctcggaggc ccccgagggt gcggatcccc
     8881 cgcgcaggca tcaccggcac cgagacaagg acaaggctcc cgccacggtc ccctcggcgg
     8941 gggaacagga cagagccgaa gctctgaggg cagagggcgg ggagctgggg ccccgcgagg
     9001 agcgggggcg gccacgccgc agccgcagca aggaggcccc gggcgccccg gaggtgcgca
     9061 gcgatcgcgg ccgtggcccg tgtcctgaag gtggtcggcg ccaccaccgg cgcgggtcac
     9121 cggaggaggc agctgagagg gagccccgcc gccaccgcgc gcaccggcac gggcccgacc
     9181 cgggcaagga gggcccagcg tccgggacca ggggcgagcg gcgcgcacga caccgcacgg
     9241 gcccccgggc gtgcccccgg gaggctgaga gcagcgagga gcctgcgcgg cggcaccggg
     9301 ctcggcacaa ggcgccgccc acccaggaga ccgcggagaa ggacaaggag gctgccgaga
     9361 aggggggcga ggccacggag gccgagaagg acaaggaggc tcggaaccac cagcccaagg
     9421 agctgccctg tgacctggag gccatcggca tgctgggagt gggcgctgtg catacgctgc
     9481 ccagcacctg tctgcagaag gtggaggagc agccagagga tgcggacaac cagcggaacg
     9541 tcacccgcat gggtagtcag cccccggaca ccagcaccac tgtgcacatt cctgtgacgc
     9601 tgaccggccc ccccggggag accacggtag tgcccagtgg aaacgtggac ctggaaagtc
     9661 aagcagaggg gaagaaggag gtggaaacca gcgatgtgat gaggagcggc cctcggccca
     9721 ttgtcccgta cagttccatg ttctgtttaa gcccaaccaa cctgctgcgc cgctgctgcc
     9781 actacattgt gaccatgcga tacttcgaga tggtcatcct cgtggtcatc gccctgagca
     9841 gcatcgccct ggctgccgag gaccccgtga gaacagactc tcccaggaat aatgctctga
     9901 agtacatgga ctacatcttc acaggcgtct tcaccttcga gatggtgata aagatgatcg
     9961 atttgggact gctgctacac cctggagcct atttccggga tctgtggaac attctggact
    10021 tcattgtggt cagtggggcc ctggtggcat ttgctttctc aggatccaaa gggaaagaca
    10081 tcagcaccat caaatccctg agagtcctgc gtgtgctgcg gccactcaag accatcaagc
    10141 ggctgcccaa gctcaaggcc gtgtttgact gtgtggtgaa ctcactgaaa aacgtcctca
    10201 acatcctgat cgtctacatg ctcttcatgt tcatctttgc cgtcattgcc gtgcagctct
    10261 tcaaagggaa gttcttctac tgcacggacg aatccaagga gctggagcgt gactgcaggg
    10321 gtcagtattt ggattatgag aaggaggaag tggaagctca accgaggcag tggaagaaat
    10381 atgacttcca ctatgacaac gtgctctggg ctctgctaac gctcttcacg gtgtccacag
    10441 gggaagggtg gcccatggtg ctgaaacact ctgtggatgc cacctacgag gagcagggcc
    10501 cgagccccgg gtaccgcatg gagctctcca tcttctacgt ggtctacttt gtggtctttc
    10561 ccttcttctt tgtcaacatc ttcgtggcct tgatcatcat caccttccag gagcaggggg
    10621 acaaggtgat gtccgagtgc agcctggaga agaacgagag ggcttgcatc gactttgcca
    10681 tcagcgccag acccctgacg cggtacatgc cccagaacaa gcagtcgttc cagtataaga
    10741 cgtggacgtt cgtggtgtcc ccaccttttg agtacttcat catggctatg atcgccctca
    10801 acactgtggt gctgatgatg aagttctacg atgcgccgta cgagtatgag ctaatgctga
    10861 aatgcctgaa catcgtgttc acgtccatgt tctccatgga gtgtgtgctg aagatcatcg
    10921 ccttcggggt gctgaactat ttcagagatg cctggaatgt ctttgacttt gtcacggtgt
    10981 tgggaagtat tactgatatt ttggtaacag agattgcgaa caacttcatc aacctcagct
    11041 tcctgcgcct gttccgggcc gcgcggctca tcaagctgct gcgccagggc tacaccatcc
    11101 gcatcctgct ttggaccttc gtgcagtcct tcaaggcgct gccctacgtg tgtctcctca
    11161 tcgccatgct cttcttcatc tacgccatca ttggcatgca ggtttttggg aacatcgccc
    11221 tggacgatga caccagcatc aacaggcaca ataacttccg gacgtttctg caggccttga
    11281 tgttgctgtt caggagcgcc acgggggagg cctggcacga gataatgctg tcgtgcctca
    11341 gcagccgggc ctgcgacgag cactccaatg ccagcgagtg cggcagcgac tttgcctact
    11401 tttacttcgt ctccttcatc ttcctctgct cctttctgat gttgaacctc tttgtggctg
    11461 tgatcatgga caattttgag taccttacac gggactcttc catcttaggg cctcaccacc
    11521 tggatgagtt catccgggtc tgggctgaat acgacccggc cgcgtgtggg cgcatcagtt
    11581 acagtgacat gtttgagatg ctgaagcaca tgtccccacc cctggggctg gggaagaagt
    11641 gccctgctcg agtcgcgtac aagcgcctgg tccgcatgaa catgcccatc tccagtgagg
    11701 acatgacggt gcacttcaca tccacgctga tggctctcat tcggacagcg ctggacatca
    11761 agctggcacc agctgggacc aagcagcacc agtgcgacgc agagctgagg aaggagattt
    11821 cctgtgtgtg ggccaatctg ccccagaaga ccctggactt actggtacca ccccataagc
    11881 ctgacgagat gacggtgggc aaagtctatg cagctctgat gatcttcgac ttctacaaac
    11941 agaacaaaac cagcagagac cagactcagc aagcccccgg aggcctgtcc cagctgggcc
    12001 ccgtgtccct gttccacccg ctgaaggcca cgctggagca gactcagccg gcgctgcgtg
    12061 gagcccgggc tttcctgcgg cagaagagtt ctgcctccct gagcaacggc ggggccgtac
    12121 aaacccagga gagcggcatc aaggaatcgg tttcttgggg cactcagagg acccaggatg
    12181 tgctttgcga ggccagggca cccctggagc ggggccactc agctgagatc ccagtcgggc
    12241 agcctgggac actggctgta gatgtccaga tgcagaacat gactctgagt ggccctgacg
    12301 ccgagccgca gccagggctg gagagccagg gccgagccgc ctccatgccc cgcctggccg
    12361 cagaaacaca gcccgcgcct gacgccagcc ccatgaagcg ctccatctcc acgctggccc
    12421 cgcgccccca cacggcccgg ctcggcagca ccgcgctgga ccgcccagcc cccagccagg
    12481 ccccgcacca tcaccaccac cgctgccacc gccgcaggga caggaagcag cggtccctgg
    12541 agaagggtcc cagcctgtct gctgacacag atggggcccc cgacagcacg gtggggccag
    12601 gactgcccac gggggagggc ccccccggct gcaggcggg
//
