LOCUS       sequence-406770-        9129 bp    DNA     circular SYN 15-APR-2025
DEFINITION  expression of rat calcium channel alpha2delta-1 with a single HA tag
            inserted after aa549 and a D491A mutation that prevents Gabapentin 
            binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Gumerov VM, Andrianova EP, Matilla MA, Page KM, Monteagudo-Cascales 
            E, Dolphin AC, Krell T, Zhulin IB
  TITLE     Amino acid sensor conserved from bacteria to humans.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Mar 8;119(10):e2110415119. doi: 
            10.1073/pnas.2110415119. Epub 2022 Mar 1.
  PUBMED    35238638
REFERENCE   2  (bases 1 to 9129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-03-8"; volume: "119(10):e2110415119. 
            doi"; pages: " 10.1073/pnas.2110415119. Epub 2022 Mar 1"
COMMENT     Sequence Label: cacna2d1 HA D491A pcDNA3
FEATURES             Location/Qualifiers
     source          1..9129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(110..130)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        115..444
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      295..430
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     357..376
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             511..1305
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(565..584)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1175..1194
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1479..1600
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1516..1535)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1570..1589
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1649..1665)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1649..1665)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1662..1684)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1673..1689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1697..1703,1704..1721,1722..1727))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1742..1763
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1880..1897)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2051..2639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2131..2150)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2810..3601,3602..3670))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3433..3452
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3671..3775)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3850..3869)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4041..4420
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4421..4624
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4575..4595
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4669..4688
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4669..4687
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6411..6437
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(8488..8506)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8489..8506)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8526..8543)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8532..8756
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(8802..9129,1..101)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8889..8908)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9099..9120
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
       61 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
      121 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      181 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      241 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
      301 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
      361 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
      421 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
      481 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
      541 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
      601 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
      661 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
      721 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
      781 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
      841 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
      901 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
      961 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     1021 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     1081 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     1141 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     1201 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     1261 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     1321 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     1381 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     1441 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     1501 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     1561 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     1621 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     1681 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     1741 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     1801 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1861 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1921 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1981 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2041 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2101 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2161 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2221 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2281 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2341 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2401 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2461 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2521 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2581 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2641 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2701 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2761 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2821 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2881 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2941 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3001 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3061 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3121 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3181 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3241 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3301 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3361 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3421 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3481 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3541 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3601 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3661 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3721 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3781 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     3841 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     3901 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     3961 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     4021 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
     4081 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     4141 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     4201 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     4261 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     4321 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     4381 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     4441 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     4501 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     4561 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     4621 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
     4681 ctatagggag acccaagctt ggtaccgagc tcggatccac tagtaacggc cgccagtgtg
     4741 ctggaattct gcagatcgcg aagatggctg ctggctgcct gctggccttg actctgacac
     4801 ttttccaatc ttggctgatc ggcccctcga gcgaggagcc cttcccttcg cccgtcacta
     4861 tcaagtcatg ggtggataaa atgcaggaag accttgtcac actggcaaag acagcaagtg
     4921 gagtgactca gcttgctgac atttatgaaa aataccaaga tttgtatact gtggagccaa
     4981 ataatgcacg ccaactggtt gaaattgcag ctagagacat tgagaaactt ctgagcaaca
     5041 gatctaaagc cctggtgcgc ctggctatgg aagcagagaa agtccaagcc gcccaccaat
     5101 ggagggaaga ttttgcaagc aacgaagttg tctactacaa cgctaaagac gatcttgatc
     5161 ctgaaagaaa tgagagtgag tcaggcagcc aacggattaa acctgttttc atcgaagacg
     5221 ctaattttgg acgccagata tcctatcaac atgcagcggt ccatattccc acggacatct
     5281 atgagggctc aaccatagtg ttaaacgaac tcaactggac aagtgcctta gatgaagttt
     5341 tcaaaagaaa tcgagatgaa gaccctacat tgctgtggca agtgtttggc agcgccaccg
     5401 gcctggcccg atattatcca gcttctccgt gggtggataa cagcagaact ccaaacaaga
     5461 tcgatctcta cgatgtacgc agaagaccat ggtacatcca gggagctgca tcccccaaag
     5521 acatgctcat tctggtggac gtgagtggaa gtgtgagcgg attgactctg aaactcatcc
     5581 ggacatctgt ctctgagatg ttagaaaccc tttccgatga tgatttcgtg aatgtagctt
     5641 catttaacag caatgctcag gatgtgagtt gtttccagca cctggttcaa gcgaatgtaa
     5701 ggaacaagaa ggtgctgaag gatgcggtga ataacatcac agcaaagggg ataacagatt
     5761 acaagaaagg ctttagcttc gcgtttgaac agctacttaa ttataatgtt tccagagcca
     5821 attgtaataa gatcatcatg ttattcacgg atggaggaga agagagagcc caggagatat
     5881 ttgccaagta caataaagac aaaaaagtcc gcgtgtttac attttctgtt ggtcaacata
     5941 attatgacag aggacctatt cagtggatgg cttgtgaaaa caaaggttat tattatgaga
     6001 ttccctctat tggtgcaata agaatcaata ctcaggaata tctagatgtt ctgggaagac
     6061 caatggtttt agccggggac aaagcaaagc aagtccagtg gacaaatgtg tatttggatg
     6121 ctctggagct gggacttgtc attactggaa ctctaccggt cttcaacgtc actggccagt
     6181 ctgaaaataa gactaacttg aagaaccagt tgattcttgg tgtgatgggc gttgctgtgt
     6241 ctctggaaga tattaagaga ttgacaccac gttttacact gtgccccaac ggttactatt
     6301 ttgcaatcga tcctaatggt tatgtcttac tgcatccaaa tcttcagcca aagaacccca
     6361 aatctcagga gccggtcaca ctggattttc tcgatgctga gttagagaat tacccttacg
     6421 acgtccctga ctacgccgat attaaagtgg agattcgaaa taaaatgata gacggagaaa
     6481 gtggagaaaa aacattcaga actctggtca aatctcaaga tgagagatat attgacaaag
     6541 gaaatcgaac atacacatgg acacctgtca atggcacaga ttacagtttg gccttggtat
     6601 tgccaaccta cagtttttac tatataaaag ccaaaataga agagacaata actcaggcca
     6661 gatcaaaaaa ggggaaaatg aaggattcag aaaccctgaa gccagacaat tttgaagaat
     6721 ctggctacac tttcatagca ccaagggaat actgcaatga cctgaaacct tcagataata
     6781 acactgaatt tcttttaaat ttcaatgaat ttattgatag gaaaactcca aacaaccctt
     6841 cctgtaatac agatttgatt aatagaatct tgctggatgc gggttttaca aatgaacttg
     6901 tccaaaatta ttggagtaaa cagaaaaata tcaaaggagt gaaggcacgc tttgtggtga
     6961 ctgatggcgg aattacaaga gtttacccca aagaggctgg agaaaactgg caagagaacc
     7021 cagagacgta tgaggacagc ttctacaaac ggagcctaga taacgataac tacgttttca
     7081 ctgcgcccta cttcaacaaa agtggacctg gtgcctatga atctggaatt atggtaagca
     7141 aagctgtaga actgtacatc caaggaaaac ttcttaagcc tgcagttgtg ggaattaaaa
     7201 ttgacgtaaa ctcctggata gaaaatttta ccaaaacttc aatcagggat ccgtgtgctg
     7261 gtccagtttg tgactgcaaa agaaacagtg atgtaatgga ctgtgtcatt ctagatgatg
     7321 gtggatttct tctgatggcc aatcatgatg attacactaa tcagattgga cgtttttttg
     7381 gagagattga cccgagcatg atgagacacc tggttaatat atcactttat gcattcaata
     7441 aatcatatga ctatcagtct gtgtgtgatc caggggcagc accaaagcaa ggagcaggac
     7501 atcgctcagc ctatgtgcca tcaattacag acatactcca gattggctgg tgggccactg
     7561 ctgccgcctg gtctattctc cagcagctac tcttgagttt gacatttcca cggctccttg
     7621 aggcagttga aatggaagag gatgacttca cagcttccct gtctaagcag agctgcatca
     7681 cagaacaaac tcagtacttc ttcaagaacg atactaaatc attcagtggt ttactggact
     7741 gtggaaactg ttccaggatc tttcatgttg agaagcttat gaacaccaac ctagtgttca
     7801 taatggtgga gagcaaggga acatgtccgt gtgacacgcg gctgctcatg caagcagaac
     7861 agacttctga tggtccagat ccttgcgaca tggttaagca gcccagatac cgaaaaggac
     7921 ctgatgtctg ctttgataac aatgtgctgg aggattatac cgactgtggt ggtgtttctg
     7981 gattaaaccc ttccttatgg tctatctttg gactccaatt tatactcctt tggctggtat
     8041 ctggcagcag acactatcta tggtgaccct ctaaacccat gatctgcaac ttaattccat
     8101 gccctgccaa aacatgagcc ctcagttaca ttaaaataga gcccgttgaa gaagctccac
     8161 agaaccttag ctgggcctct accctgatgt aactgtgagg cttagattcc tgaccatcgc
     8221 tggctgcatg tcagggtgtt ggaaactgaa actcgtgtga acatgctgca tcatctgtgt
     8281 ccaacatcaa agcaaagctc tactcattct ctgtgggagg tttgggagtt cgctgttgca
     8341 ctgttggaga ttacgtggaa aaggctcccc cacgcaggcc ttatgaaaag aagcagtctt
     8401 gactttgacc tagcatttca acctgctgca gttttaccaa gccgaattcg catgcggccg
     8461 ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg
     8521 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     8581 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     8641 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     8701 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     8761 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     8821 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     8881 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     8941 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     9001 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     9061 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     9121 aacaacact
//
