LOCUS       sequence-407425-        6219 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Mammalian expression of Junin virus GPC protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_JUNV_GPC
FEATURES             Location/Qualifiers
     source          1..6219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1419..1428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2924..2943)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2989..3044
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3043..3062)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3402..3418)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3402..3418)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3415..3437)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3426..3442
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3450..3456,3457..3474,3475..3480))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3495..3516
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3633..3650)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3804..4392)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3884..3903)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4563..5354,5355..5423))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5186..5205
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5424..5528)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5554..6009)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5691..5712)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5903..5922
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6136..6158
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6150..6167
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6151..6167
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6177..6196
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6177..6195
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatggga cagttcatca
     1441 gctttatgca agagattccc acatttctgc aagaggctct gaatattgcc ctcgtcgccg
     1501 tgtctctgat tgccatcatc aagggcatcg tcaatctgta caagagcgga ctgtttcagt
     1561 tctttgtgtt tctggccctc gccggaagaa gctgtaccga ggaagccttc aaaatcggac
     1621 tgcataccga gttccagacc gtcagcttct ccatggtcgg cctcttcagc aacaatcctc
     1681 acgacctccc tctgctgtgt acactgaaca agagccacct ctacatcaag ggaggcaacg
     1741 cctccttcca gatcagcttt gacgacattg ccgtgctgct gcctcaatac gacgtcatca
     1801 ttcaacaccc cgccgatatg agctggtgct ccaaaagcga cgaccagatt tggctctccc
     1861 agtggttcat gaacgctgtc ggccacgatt ggcatctgga tccccctttt ctgtgcagaa
     1921 atagaaccaa gaccgagggc ttcatcttcc aagtgaacac cagcaagacc ggcgtgaacg
     1981 aaaactatgc caagaagttc aagaccggaa tgcaccatct gtacagagaa taccccgaca
     2041 gctgccccaa tggcaagctg tgtctgatga aggctcagcc caccagctgg cctctgcagt
     2101 gtcctctgga tcacgtgaat acactgcact tcctcacaag aggaaagaac attcagctgc
     2161 ctaggaggtc tctgaaggcc tttttcagct ggtctctgac agacagcagc ggaaaagaca
     2221 cccccggcgg ctattgtctc gaggagtgga tgctggtcgc cgctaagatg aaatgcttcg
     2281 gcaataccgc cgtggccaaa tgtaatctga accacgactc cgagttctgt gatatgctga
     2341 ggctgttcga ctacaacaag aacgctatca agacactgaa cgacgagacc aagaagcaag
     2401 tgaatctgat gggccaaacc attaacgctc tgatcagcga taatctgctc atgaaaaata
     2461 agattagaga gctcatgagc gtgccttatt gcaactacac caaattctgg tacgtgaacc
     2521 acacactgag cggacaacac tctctgccta ggtgctggct gatcaagaac aactcctatc
     2581 tcaatattag cgactttaga aacgactgga tcctcgagag cgactttctg atcagcgaga
     2641 tgctcagcaa ggagtatagc gataggcaag gcaagacacc tctcacactg gtggacatct
     2701 gcttctggtc caccgtgttc ttcacagcca gcctctttct gcatctggtg ggcatcccca
     2761 cacatagaca tattagagga gaagcttgcc ctctgcctca tagactcaac tctctgggag
     2821 gatgtagatg cggaaaatac cccaatctga aaaagcccac cgtgtggaga agaggacatt
     2881 gatgtcttcc tggagctcca gtcattgact ataacgtctc agatcttttt ccctctgcca
     2941 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
     3001 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     3061 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     3121 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
     3181 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     3241 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     3301 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
     3361 tatggagatc cctcgacgga tcggccgcaa ttcgtaatca tgtcatagct gtttcctgtg
     3421 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     3481 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     3541 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     3601 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3661 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3721 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3781 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3841 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3901 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3961 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4021 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4081 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4141 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4201 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4261 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4321 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4381 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4441 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4501 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4561 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4621 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4681 cccagtgctg caatgatacc gcgagaccca cgctcaccgg caccagattt atcagcaata
     4741 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4801 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4861 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4921 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     4981 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5041 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5101 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5161 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5221 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5281 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5341 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5401 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     5461 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5521 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     5581 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     5641 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     5701 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     5761 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     5821 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     5881 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     5941 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     6001 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     6061 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     6121 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     6181 acgactcact atagggcgaa ttggagctcc accgcggtg
//
