LOCUS       sequence-407431-        9003 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Express mEGFP-tagged fusion protein, NCOA4_RET from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9003)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_NCOA4_RET
FEATURES             Location/Qualifiers
     source          1..9003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(3764..4474,4475..4477,4478..4480))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3806..3827)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4155..4174
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4414..4435
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4477..4486
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4562..4765)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4591..4611)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4766..5069
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5326..5370)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5555..5788
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6148..6265
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6757..6882
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6929..7186
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7188..7567
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7598..7702
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7703..7771,7772..8563)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7921..7940)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8734..9003,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcagc
     1801 tgtcaaaagt gtccattaat tttgctgcgg acggactcaa catccaatta gcgtagacgc
     1861 tgtcattagg ataacgggga aaaccagtat ttgtgccatc tgcacgcgta agtggaacag
     1921 gagactcacc cggccagtta ggatcactca tcccatacaa cttattttcg atccaggttg
     1981 agggcagggc gcggggcaag ggagcattgt tgcagtctac cagtggcgtt tcttcctcgg
     2041 ataagccgtc atcgtagata aggctatcac ttggcgtgct ggctgctaaa tccaaataat
     2101 cacgacgttt caccatcatt ttttccaaat cctttgagat gtcagcgaag acaggacgtt
     2161 tgtcgggctc ctgtttccaa cactgcaaca ttaagcgata catttcttca gagcaattat
     2221 cggggcgctc catacggtgt cctgttttta aaaggttgaa caaacgctcg ggcggaatac
     2281 ctgggtaggg attgccacct aaagtcacaa tctcccaaag caagacaccg aatgaccaga
     2341 cgtcactctg cgttgtgtag atgtgatcaa acaaactctc aatagccatc cacttgactg
     2401 ggatacgtcc ctgactacgc ttaacataac tatcttcctc gtatacgtca cgtgacagtc
     2461 caaaatcgct gatcttcatt ttacgccctt cagctacaag gatattacgc gctgcaagat
     2521 cgcggtgtac cagcttcatt tctgccaggt attgcatacc ctgtgaaatc tgccacgcaa
     2581 aggaaatcag atcccccatt gtcaatgcgc gttcatccgg gtgatctaat gaactcgaat
     2641 tacgggaccc gcctgatccc aagtaaccag gcccaacctt acgagattcg cgtaagaacc
     2701 cgcgaagact gccgtatttg gcgtactcga cgattaacag aagcggtcca tcctgactac
     2761 atgcgccata taatttaatc acatgtggat ggttcacttg tttaagtaca ttaaactcgg
     2821 acagcaggtc acgcaattca gacgggctgg cgttttcttt caacattttt accgcgacag
     2881 ttgtatagcc tgcgcgacct ttaaggtgaa aagctgtagc tttcacgact ttgccaaact
     2941 ccccttcccc caatgtctta cctaacacaa ggtttttgcg aggaaattcc catttcggat
     3001 cttcctgcga attttctaac gtctgttttt gagtcagcca gtcctggggg tcggtactag
     3061 gaatgtaagg ggcttggtaa ccagaggccg gtttacttcc taaaagccac tctgaaaatg
     3121 gaacggcgac aatacccgaa gctgatttct gctccggcat tgagatacat ccgcgttttt
     3181 ccaggaaggg gccgatgttg gcactggagg cgtgtgccat caagtgctct ggaatttgga
     3241 tggtcttcaa cgagccaaaa gtggtgattg tttgacgcag cgtaatggta tccgcttcaa
     3301 acagaagtac agtcgaatct tctggtttca acgttaacga gcccaagcgc tctaagcaca
     3361 cgctaacctg attcgctaag tccttgttct gcgtgcattc aagttggtgt gttaagcaat
     3421 taaattgacc cagcaaagaa taaagttgct gggcttgctg ctgaagtgtt tcctctttaa
     3481 gttggtaaat aaggtccacc tgttcatata accacacttc acgactacgt aaacactcca
     3541 gatgacggct aatgcagcta tggatctgag ccttaacctc acgcagattg tccttgattt
     3601 gttgttctgc acgcaaaacc ccaccaatgg caagttccag gtcgcgacgt gcgtcagagc
     3661 aacgcagcaa tggttcgcga ttggagctac tacctgattg gtcttgaaaa gtattcattc
     3721 cccgggaagc ggccgcactt cccccggagc ctccgcttcc gcccttgtac agctcgtcca
     3781 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
     3841 cgttggggtc tttgctcagc ttggactggg tgctcaggta gtggttgtcg ggcagcagca
     3901 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
     3961 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
     4021 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
     4081 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
     4141 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
     4201 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
     4261 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
     4321 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
     4381 cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
     4441 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggtggcgacc ggtggatccc
     4501 gggcccgcgg taccgtcgac tgcagaattc ggttcactaa acgagcccgg ttcactaaac
     4561 gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
     4621 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
     4681 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
     4741 tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact
     4801 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     4861 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     4921 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     4981 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     5041 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     5101 accccgtaat tgattactat taataactag cacgcgttcg aagcttgagc tcggtcccct
     5161 cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac
     5221 tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa
     5281 taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt
     5341 atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa
     5401 ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg
     5461 attccaaatc tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt
     5521 gcaaatgagt tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct
     5581 ttccacagcc aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag
     5641 atgctgttgc gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa
     5701 taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc
     5761 tcccaagaac ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac
     5821 tcttctcttt gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt
     5881 atataattca cttctccaat tgtccctcat atctcctcct tatggtgttt tactaaactt
     5941 ttccatgttc taatcctcat cctgtctact tgccacacaa tcatcacctg ccatctgttt
     6001 tccataatcc ctaatgatct ttgcttttct tcttggcact acttttatgt cactattatc
     6061 ttgtattact actgcccctt cacctttcca gaggagcttt gctggtcctt tccaaagtgg
     6121 atttctgctg tccctgtaat aaacccgaaa attttgaatt tttgtaattt gtttttgtaa
     6181 ttctttagtt tgtatgtctg ttgctattat gtctactatt ctttcccctg cactgtaccc
     6241 cccaatcccc ccttttcttt taaaattgtg gatgaatact gccatttgta ctgctgtctt
     6301 aagatgttca gcctgatctc ttacctgtcc tataattttc tttaattctt tattcataga
     6361 ttctactact ccttgacttt ggggattgta gggaatttcc aggtctgaag atcctgctgc
     6421 ttgctgtgct tttttcttac ttttgttttg ctcttcctct atcttgtcta aagcttcctt
     6481 ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg tattatataa
     6541 tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca gtatttgtct
     6601 acagccttct gatgtttcta acaggccagt attaactgcg aatcgttcta gctccctgct
     6661 tgcccatact atatgtttta atttatattt tttctttccc cctggcctta accgaatttt
     6721 ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc catctctctc
     6781 cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc cctcgcctct
     6841 tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg gtttcccttt
     6901 cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt
     6961 ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact
     7021 caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag
     7081 gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg cttatatgca
     7141 ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctcccat ggtaatagcg
     7201 atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca
     7261 taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga
     7321 tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa
     7381 tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg
     7441 gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc
     7501 atatatgggc tatgaactaa tgaccccgta attgattact attaataact agtcaataat
     7561 caatgtcgac ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     7621 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     7681 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     7741 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     7801 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     7861 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     7921 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     7981 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     8041 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     8101 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     8161 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     8221 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     8281 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     8341 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     8401 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     8461 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     8521 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     8581 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     8641 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8701 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8761 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     8821 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8881 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     8941 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     9001 cgg
//
