LOCUS       sequence-407453-        9420 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Express mEGFP-tagged fusion protein, RUNX1_RUNX1T1 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_RUNX1_RUNX1T1
FEATURES             Location/Qualifiers
     source          1..9420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4181..4891,4892..4894,4895..4897))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4223..4244)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4572..4591
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4831..4852
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4894..4903
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4979..5182)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5008..5028)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5183..5486
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5743..5787)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5972..6205
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6565..6682
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7174..7299
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7346..7603
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7605..7984
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8015..8119
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8120..8188,8189..8980)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8338..8357)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9151..9420,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcaac
     1801 ggggggttgt ctcgatagtc gaaggcgtcc ctggggttgt actacggggt gttgccgccg
     1861 gtggagtatc cattgggctc ccagcgccgg aattcggggt gacactagaa ctgacagcgg
     1921 gagtatctcc ttgctgttga gcctgcaatg tttgtccgca gatgtggtgg tgcttttccc
     1981 agtccttgtg ttgacagaag ctcccgcaat aacgtgccgt attgcagccg ctgcaagtct
     2041 cacttgcctt acgtccgcaa ttccagcaac tctcacttga atcttcttgc tgattaatta
     2101 cagccaaagc atcctcggcc gcctggcgct tcgcctccgc gactgtgcgt tccatcttag
     2161 cgcgctccgt tgtaatcata tcatgtgctt tgcgttcagc ctcactaact gctttttgta
     2221 attcggtcat agcctggcgt ttaacctcgt tgactgcctc ttctgctttc ttccaaatct
     2281 cctcaggaac atacccacta gcagggcggt gcagaaactc acggtgcgca tctaaggcga
     2341 ccgggtcggg attaacggga gattgctgac gggagtggct tgaggagcta ccaccaccct
     2401 tctttaaatc ctccgcatct gagtagcgac gaatccaata gttcagctct tcacggtccg
     2461 cttcttgaca gcggcgcagc acggttaagg aacggcgcgt tttttccacc atatccataa
     2521 tgcagttaag cagatgatca agatgcttcc attcttctgc ccattcacgg tctgtcaaac
     2581 gatggtcgat catctcttct tgacgtgttc cgtgcaatcc catcggacga ttacgatcac
     2641 gaagatcacg atgggatgga tggcgatagg agtcgcggta atggtgcgcg attgccatgt
     2701 cgtccaaacg ataatgttgg ggcggcggtg gtgtaggatg aggaagtcca ttgggctggt
     2761 aactaagacc attattcggt gagtaacgct ggcctgggct gatggtacac ggacgtttcg
     2821 aaggatgttc agagtgcaat ggttcacgat caaagccgtt ctcttttgtg cgatcgggag
     2881 tgcggcgttt accgttttcg tttacgtcca aaagtaactc actactgtct actggagaag
     2941 ttgtactagc gtccaacaaa agctgctcgt gctgcgcaag atattgagcg ggattctgtt
     3001 tcgctaaacg tgcacaatgc aacagttcac gttgcaacag tggcaagttc gcctttaaga
     3061 atggaataac aaaaggacgt aaagggaaat tcgttgcttc ttgcagttta gaatggaact
     3121 cttcgatcgt aagggtagaa ttcaccaaac ccaaaaccaa tgtgcgaaca cgctctccaa
     3181 tttcgggact gatatcatta ccgaattgct gtaatgtcgt aaggaaacgc ttcagcttgc
     3241 taagctggcg cgcgccacag gctggaggaa gttgctgatt agcaagggat gaggaggagg
     3301 aggatgaagg cccattggaa aacccatttg gcggtgatgg tgcaccatta agagcagtag
     3361 gagaatggct ggtgccgtta gtcaaagtgg taggagtaaa actagaggtg cgaggcgccc
     3421 cctgggttgt tgggggtggc ggcatcgttg gcggtgtcaa gcgactttga gtcttcacgt
     3481 caactgggga atccggcatt gtggaatgct tttctgtacg gttacgcggc tcacgtgggc
     3541 cgtcaacggt gatcttgatt gcgcgatgat atgttgccac ttgcggcggg tttgtaaaga
     3601 ctgtgatggt taaggtgaat gatttcccac gcccactgcg acccacgaaa cgcagatcgt
     3661 tgaaacgtgc aacctgattc ttcattgctg cggtagcgtt acgcaactcc gcactgtaat
     3721 tttcatcgtt gcctgccatc actgttacca aagtaccgtc aggcacatca cccagcgcta
     3781 caaccttgaa cgcgattggc aaggttttat tgcagcgcca gtgtgtgggt aacacactgc
     3841 ataaaaaatt aggactatcc gtacgtacaa gctcaccggg atggtccgcc agaacttcaa
     3901 ccatactacg atccccgcta cgcagtttgc cggccagtgc agcaccggcg tcgggtgcgc
     3961 caaggggcaa cgcttccgac attttgcccg gagacaaggc ggtcgaggga ggggtaaagc
     4021 ggcgcgaagt actcgcatcg tgcacgtcac gcgacgggtt cattcctaaa atgcactcgc
     4081 gcatgaagca ttggggatat gaaggaaaag attcgaagat actatctgag gccattcccc
     4141 gggaagcggc cgcacttccc ccggagcctc cgcttccgcc cttgtacagc tcgtccatgc
     4201 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
     4261 tggggtcttt gctcagcttg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg
     4321 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct
     4381 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga
     4441 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct
     4501 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
     4561 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
     4621 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc
     4681 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct
     4741 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
     4801 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
     4861 ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt ggatcccggg
     4921 cccgcggtac cgtcgactgc agaattcggt tcactaaacg agcccggttc actaaacgag
     4981 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     5041 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     5101 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     5161 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     5221 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     5281 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     5341 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     5401 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     5461 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     5521 ccgtaattga ttactattaa taactagcac gcgttcgaag cttgagctcg gtcccctcgg
     5581 ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat
     5641 agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa
     5701 ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc
     5761 taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga
     5821 gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt
     5881 ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag cagtggtgca
     5941 aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc
     6001 cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg
     6061 ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa
     6121 ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc
     6181 caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct
     6241 tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata
     6301 taattcactt ctccaattgt ccctcatatc tcctccttat ggtgttttac taaacttttc
     6361 catgttctaa tcctcatcct gtctacttgc cacacaatca tcacctgcca tctgttttcc
     6421 ataatcccta atgatctttg cttttcttct tggcactact tttatgtcac tattatcttg
     6481 tattactact gccccttcac ctttccagag gagctttgct ggtcctttcc aaagtggatt
     6541 tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc
     6601 tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc
     6661 aatcccccct tttcttttaa aattgtggat gaatactgcc atttgtactg ctgtcttaag
     6721 atgttcagcc tgatctctta cctgtcctat aattttcttt aattctttat tcatagattc
     6781 tactactcct tgactttggg gattgtaggg aatttccagg tctgaagatc ctgctgcttg
     6841 ctgtgctttt ttcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt
     6901 gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga
     6961 tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca
     7021 gccttctgat gtttctaaca ggccagtatt aactgcgaat cgttctagct ccctgcttgc
     7081 ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc
     7141 ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt
     7201 ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc
     7261 cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc
     7321 tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg
     7381 agggatctct agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa
     7441 ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct
     7501 cagatctggt ctaaccagag agacccagta caggcaaaaa gcagctgctt atatgcagga
     7561 tctgagggct cgccactccc cagtcccgcc caggccacgc ctcccatggt aatagcgatg
     7621 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
     7681 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
     7741 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
     7801 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
     7861 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
     7921 tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa
     7981 tgtcgaccca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     8041 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     8101 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     8161 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8221 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8281 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8341 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     8401 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8461 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8521 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8581 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8641 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8701 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8761 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8821 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8881 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8941 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     9001 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9061 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9121 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9181 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9241 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     9301 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9361 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
//
