LOCUS       sequence-407488-        9051 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express mEGFP-tagged fusion protein, VAV2_COL27A1 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9051)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_VAV2_COL27A1
FEATURES             Location/Qualifiers
     source          1..9051
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(3812..4522,4523..4525,4526..4528))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3854..3875)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4203..4222
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4462..4483
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4525..4534
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4610..4813)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4639..4659)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4814..5117
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5374..5418)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5603..5836
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6196..6313
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6805..6930
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6977..7234
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7236..7615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7646..7750
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7751..7819,7820..8611)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7969..7988)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8782..9051,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcaca
     1801 gaaaacacgc cggacccacc tccagacgat actgcttacc agacgaagcc ggcggaaggt
     1861 tatcaacgct gataatgggc agttgctgcg ggtcctgggt gcgaaaggta aacagcgtct
     1921 gatgccagcg accgtcctgc accttgcagc cgtccatgct tacctccggg cgaaattgac
     1981 cgccggcttc aaagatctga ccattccatg cacgaaaacg caccgcttgc ttagccggag
     2041 tttgacccgt accctcctgc caaaccgtca tattcaagca atggatggta atatgctggg
     2101 tcacttctga agacagcagg tgaagaaagt tcatctgaac acggctaatc gcaaattcca
     2161 ctttgctggc agtaatcggt ttcaggcacg tttgaccacc atgggtgaaa ttacaggata
     2221 cctcaatggt gtcgctgcta caacccaagt tggggtcaac ccaataggtg ccatcaacca
     2281 ttttctgttc acaatccatc aggtcacggc agacacgcgc cgggttctct ttggtgccca
     2341 gcggggtttt aatgctttgg attaagttgg acaggtagtg cagggtcttg aagatctcac
     2401 ccccctgatc gagcaccaga cgatccgggt agctgtagct ctccggacgc agcgcaccgc
     2461 tggtatccat ccaggtctga aatgcggcgc cgagatcgtc ttgttgaagt tggatcggac
     2521 cacccggcgg acctggcgga cctggtctac cgcgaggacc tggcgggccg cgcgggcctt
     2581 gcaaacccca gtcgccacga gatcctttgt cacccttcgg ccccggcaga ccactagggc
     2641 ccaaaatgcc caacgggccg acgatgccct ctttacccat caggcccggc ggaccacgcg
     2701 gcccctggat gcctttgaaa ccgatatcgc ccggaggtcc ctttgggccg aataaacctg
     2761 ccatgcccgg gaaacccatc tcgccgctgt cgcccggctg acctttggag ccaggctctc
     2821 cctggttacc cgggagaccg gtacgccctt ccgtacctct cttgcccgga ggacccaatt
     2881 gacccggcaa accggactca cccttgaagc ccggcggacc ctgcgcgccc ggcaggcctg
     2941 cgacacccgg attaccgcgc ttgcccgctg gtcccatcgg gcccgggtcg ccgtcatcac
     3001 cttgctcacc ttgttgacca gcttggccgt ctctgcctgg caggccgtcg gggcccgcga
     3061 tgccttccag accctggcgg cccacaacgc cacgcggacc cggcagaccc tgcagacctt
     3121 gcacgccacg gcgccccgga gcacccgcct ttccctgctt acctttcggg ccttcagcgc
     3181 ctttggaacc cttcggaccc ggccagccac gcgggcccgg gtgaccgggt tgaccttgtt
     3241 gacccggttt accacgcagg ccctgcacac cttcttggcc cggataaccc ggatcacccg
     3301 gttcaccacg atcgccacga tctcctaccg gaccgcggtc gcccggcgga cctggtgggc
     3361 cttccgcctt cccgtcttcg ccttgctcgc ctttctcgcc cttcgggccc ggcggaccca
     3421 acggacccac gatgcctttg tgacctttac cgaagtcacg aacgtcgaac aggtcaaacg
     3481 gatcgaacag ttcgctgtta cgcagaccga atttatcgtg gcacacctta aggaaggtac
     3541 gaatgttctt caggcacagg aactggctca tctgcgggcg gaagttgata tctttcagat
     3601 caatagagcc cggcgacaag ttgtgcagga gctggcataa cagcacaccg tcgcgcagcg
     3661 cctgcgccag gtcaaaaaca actgcgctag gccaaacaac acgatgattc ggcggcagga
     3721 ccttgcaatc tatcagccat cttccacatt gcctccactg ttccattccc cgggaagcgg
     3781 ccgcacttcc cccggagcct ccgcttccgc ccttgtacag ctcgtccatg ccgagagtga
     3841 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
     3901 tgctcagctt ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
     3961 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
     4021 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
     4081 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
     4141 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
     4201 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
     4261 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact
     4321 gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
     4381 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
     4441 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
     4501 tgaacagctc ctcgcccttg ctcaccatgg tggcgaccgg tggatcccgg gcccgcggta
     4561 ccgtcgactg cagaattcgg ttcactaaac gagcccggtt cactaaacga gctctgctta
     4621 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
     4681 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
     4741 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
     4801 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     4861 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     4921 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     4981 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     5041 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     5101 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
     5161 attactatta ataactagca cgcgttcgaa gcttgagctc ggtcccctcg gggttgggag
     5221 gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac
     5281 agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata attttatata
     5341 ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa
     5401 taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa
     5461 gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg
     5521 ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt
     5581 tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag
     5641 gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc
     5701 ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc
     5761 ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc
     5821 aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc
     5881 cttggtgggt gctactccta atggttcaat ttttactact ttatatttat ataattcact
     5941 tctccaattg tccctcatat ctcctcctta tggtgtttta ctaaactttt ccatgttcta
     6001 atcctcatcc tgtctacttg ccacacaatc atcacctgcc atctgttttc cataatccct
     6061 aatgatcttt gcttttcttc ttggcactac ttttatgtca ctattatctt gtattactac
     6121 tgccccttca cctttccaga ggagctttgc tggtcctttc caaagtggat ttctgctgtc
     6181 cctgtaataa acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg
     6241 tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc
     6301 ttttctttta aaattgtgga tgaatactgc catttgtact gctgtcttaa gatgttcagc
     6361 ctgatctctt acctgtccta taattttctt taattcttta ttcatagatt ctactactcc
     6421 ttgactttgg ggattgtagg gaatttccag gtctgaagat cctgctgctt gctgtgcttt
     6481 tttcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat
     6541 ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc
     6601 ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga
     6661 tgtttctaac aggccagtat taactgcgaa tcgttctagc tccctgcttg cccatactat
     6721 atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga
     6781 tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc
     6841 gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc
     6901 ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc
     6961 cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct gagggatctc
     7021 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
     7081 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
     7141 tctaaccaga gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc
     7201 tcgccactcc ccagtcccgc ccaggccacg cctcccatgg taatagcgat gactaatacg
     7261 tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg
     7321 ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt
     7381 actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct
     7441 attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc
     7501 ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta
     7561 tgaactaatg accccgtaat tgattactat taataactag tcaataatca atgtcgaccc
     7621 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7681 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7741 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7801 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7861 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7921 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7981 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8041 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8101 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8161 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8221 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8281 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8341 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8401 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8461 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8521 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8581 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8641 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8701 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8761 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8821 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8881 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8941 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9001 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg g
//
