LOCUS       sequence-407733-       12554 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression of human ROS1 receptor tyrosine kinase in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12554)
  AUTHORS   Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, 
            Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova 
            Bosakova M, Trantirek L, Mayer J, Krejci P
  TITLE     One reporter for in-cell activity profiling of majority of protein 
            kinase oncogenes.
  JOURNAL   Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536.
  PUBMED    28199182
REFERENCE   2  (bases 1 to 12554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-15"; volume: "6. pii: e21536. doi"; pages: " 
            10.7554/eLife.21536"
COMMENT     Sequence Label: pcDNA3.1-ROS1-V5/HIS
FEATURES             Location/Qualifiers
     source          1..12554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1287..1328
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1338..1355
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1378..1395)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1384..1608
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1654..2082
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1741..1760)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1951..1972
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2091..2111)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2096..2425
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2276..2411
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2338..2357
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2492..3286
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2546..2565)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3156..3175
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3462..3583
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3499..3518)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3553..3572
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3632..3648)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3632..3648)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3645..3667)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3656..3672
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3680..3686,3687..3704,3705..3710))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3725..3746
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3863..3880)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4034..4622)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4114..4133)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4793..5584,5585..5653))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5416..5435
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5654..5758)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5833..5852)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6024..6403
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6404..6607
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6558..6578
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6652..6671
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6652..6670
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aaaaactgac tctgcgtctc ttgctgggaa gtggagcctt tggagaagtg tatgaaggaa
       61 cagcagtgga catcttagga gttggaagtg gagaaatcaa agtagcagtg aagactttga
      121 agaagggttc cacagaccag gagaagattg aattcctgaa ggaggcacat ctgatgagca
      181 aatttaatca tcccaacatt ctgaagcagc ttggagtttg tctgctgaat gaaccccaat
      241 acattatcct ggaactgatg gagggaggag accttcttac ttatttgcgt aaagcccgga
      301 tggcaacgtt ttatggtcct ttactcacct tggttgacct tgtagacctg tgtgtagata
      361 tttcaaaagg ctgtgtctac ttggaacgga tgcatttcat tcacagggat ctggcagcta
      421 gaaattgcct tgtttccgtg aaagactata ccagtccacg gatagtgaag attggagact
      481 ttggactcgc cagagacatc tataaaaatg attactatag aaagagaggg gaaggcctgc
      541 tcccagttcg gtggatggct ccagaaagtt tgatggatgg aatcttcact actcaatctg
      601 atgtatggtc ttttggaatt ctgatttggg agattttaac tcttggtcat cagccttatc
      661 cagctcattc caaccttgat gtgttaaact atgtgcaaac aggagggaga ctggagccac
      721 caagaaattg tcctgatgat ctgtggaatt taatgaccca gtgctgggct caagaacccg
      781 accaaagacc tacttttcat agaattcagg accaacttca gttattcaga aattttttct
      841 taaatagcat ttataagtcc agagatgaag caaacaacag tggagtcata aatgaaagct
      901 ttgaaggtga agatggcgat gtgatttgtt tgaattcaga tgacattatg ccagttgctt
      961 taatggaaac gaagaaccga gaagggttaa actatatggt acttgctaca gaatgtggcc
     1021 aaggtgaaga aaagtctgag ggtcctctag gctcccagga atctgaatct tgtggtctga
     1081 ggaaagaaga gaaggaacca catgcagaca aagatttctg ccaagaaaaa caagtggctt
     1141 actgcccttc tggcaagcct gaaggcctga actatgcctg tctcactcac agtggatatg
     1201 gagatgggtc tgataagggt caagacaatt ctgcagatat ccagcacagt ggcggccgct
     1261 cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg
     1321 attctacgcg taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct
     1381 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     1441 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     1501 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     1561 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     1621 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     1681 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     1741 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     1801 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     1861 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     1921 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     1981 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     2041 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     2101 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     2161 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     2221 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     2281 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     2341 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     2401 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     2461 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     2521 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     2581 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     2641 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     2701 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     2761 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     2821 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     2881 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     2941 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     3001 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     3061 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     3121 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     3181 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     3241 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     3301 ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     3361 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     3421 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     3481 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3541 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     3601 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3661 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     3721 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     3781 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3841 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3901 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3961 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4021 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4081 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4141 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4201 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4261 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4321 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4381 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4441 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4501 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4561 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4621 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4681 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4741 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4801 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4861 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4921 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4981 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5041 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5101 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5161 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5221 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5281 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5341 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5401 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5461 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5521 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5581 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5641 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5701 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5761 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     5821 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     5881 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     5941 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     6001 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
     6061 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     6121 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     6181 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     6241 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     6301 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     6361 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     6421 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     6481 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     6541 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     6601 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
     6661 tcactatagg gagacccaag ctggctagtt aagcttggta ccgagctcgg atccagtacc
     6721 cttcaccatg aagaacattt actgtcttat tccgaagctt gtcaattttg caactcttgg
     6781 ctgcctatgg atttctgtgg tgcagtgtac agttttaaat agctgcctaa agtcgtgtgt
     6841 aactaatctg ggccagcagc ttgaccttgg cacaccacat aatctgagtg aaccgtgtat
     6901 ccaaggatgt cacttttgga actctgtaga tcagaaaaac tgtgctttaa agtgtcggga
     6961 gtcgtgtgag gttggctgta gcagcgcgga aggtgcatat gaagaggaag tactggaaaa
     7021 tgcagaccta ccaactgctc cctttgcttc ttccattgga agccacaata tgacattacg
     7081 atggaaatct gcaaacttct ctggagtaaa atacatcatt cagtggaaat atgcacaact
     7141 tctgggaagc tggacttata ctaagactgt gtccagaccg tcctatgtgg tcaagcccct
     7201 gcaccccttc actgagtaca ttttccgagt ggtttggatc ttcacagcgc agctgcagct
     7261 ctactcccct ccaagtccca gttacaggac tcatcctcat ggagttcctg aaactgcacc
     7321 tttgattagg aatattgaga gctcaagtcc cgacactgtg gaagtcagct gggatccacc
     7381 tcaattccca ggtggaccta ttttgggtta taacttaagg ctgatcagca aaaatcaaaa
     7441 attagatgca gggacacaga gaaccagttt ccagttttac tccactttac caaatactat
     7501 ctacaggttt tctattgcag cagtaaatga agttggtgag ggtccagaag cagaatctag
     7561 tattaccact tcatcttcag cagttcaaca agaggaacag tggctctttt tatccagaaa
     7621 aacttctcta agaaagagat ctttaaaaca tttagtagat gaagcacatt gccttcggtt
     7681 ggatgctata taccataata ttacaggaat atctgttgat gtccaccagc aaattgttta
     7741 tttctctgaa ggaactctca tatgggcgaa gaaggctgcc aacatgtctg atgtatctga
     7801 cctgagaatt ttttacagag gttcaggatt aatttcttct atctccatag attggcttta
     7861 tcaaagaatg tatttcatca tggatgaact ggtatgtgtc tgtgatttag agaactgctc
     7921 aaacatcgag gaaattactc caccctctat tagtgcacct caaaaaattg tggctgattc
     7981 atacaatggg tatgtctttt acctcctgag agatggcatt tatagagcag accttcctgt
     8041 accatctggc cggtgtgcag aagctgtgcg tattgtggag agttgcacgt taaaggactt
     8101 tgcaatcaag ccacaagcca agcgaatcat ttacttcaat gacactgccc aagtcttcat
     8161 gtcaacattt ctggatggct ctgcttccca tctcatccta cctcgcatcc cctttgctga
     8221 tgtgaaaagt tttgcttgtg aaaacaatga ctttcttgtc acagatggca aggtcatttt
     8281 ccaacaggat gctttgtctt ttaatgaatt catcgtggga tgtgacctga gtcacataga
     8341 agaatttggg tttggtaact tggtcatctt tggctcatcc tcccagctgc accctctgcc
     8401 aggccgcccg caggagcttt cggtgctgtt tggctctcac caggctcttg ttcaatggaa
     8461 gcctcctgcc cttgccatag gagccaatgt catcctgatc agtgatatta ttgaactctt
     8521 tgaattgggc ccttctgcct ggcagaactg gacctatgag gtgaaagtat ccacccaaga
     8581 ccctcctgaa gtcactcata ttttcttgaa cataagtgga accatgctga atgtacctga
     8641 gctgcagagt gctatgaaat acaaggtttc tgtgagagca agttctccaa agaggccagg
     8701 cccctggtca gagccctcag tgggtactac cctggtgcca gctagtgaac caccatttat
     8761 catggctgtg aaagaagatg ggctttggag taaaccatta aatagctttg gcccaggaga
     8821 gttcttatcc tctgatatag gaaatgtgtc agacatggat tggtataaca acagcctcta
     8881 ctacagtgac acgaaaggcg acgtttttgt gtggctgctg aatgggacgg atatctcaga
     8941 gaattatcac ctacccagca ttgcaggagc aggggcttta gcttttgagt ggctgggtca
     9001 ctttctctac tgggctggaa agacatatgt gatacaaagg cagtctgtgt tgacgggaca
     9061 cacagacatt gttacccacg tgaagctatt ggtgaatgac atggtggtgg attcagttgg
     9121 tggatatctc tactggacca cactctattc agtggaaagc accagactaa atggggaaag
     9181 ttcccttgta ctacagacac agccttggtt ttctgggaaa aaggtaattg ctctaacttt
     9241 agacctcagt gatgggctcc tgtattggtt ggttcaagac agtcaatgta ttcacctgta
     9301 cacagctgtt cttcggggac agagcactgg ggataccacc atcacagaat ttgcagcctg
     9361 gagtacttct gaaatttccc agaatgcact gatgtactat agtggtcggc tgttctggat
     9421 caatggcttt aggattatca caactcaaga aataggtcag aaaaccagtg tctctgtttt
     9481 ggaaccagcc agatttaatc agttcacaat tattcagaca tcccttaagc ccctgccagg
     9541 gaacttttcc tttaccccta aggttattcc agattctgtt caagagtctt catttaggat
     9601 tgaaggaaat gcttcaagtt ttcaaatcct gtggaatggt ccccctgcgg tagactgggg
     9661 tgtagttttc tacagtgtag aatttagtgc tcattctaag ttcttggcta gtgaacaaca
     9721 ctctttacct gtatttactg tggaaggact ggaaccttat gccttattta atctttctgt
     9781 cactccttat acctactggg gaaagggccc caaaacatct ctgtcacttc gagcacctga
     9841 aacagttcca tcagcaccag agaaccccag aatatttata ttaccaagtg gaaaatgctg
     9901 caacaagaat gaagttgtgg tggaatttag gtggaacaaa cctaagcatg aaaatggggt
     9961 gttaacaaaa tttgaaattt tctacaatat atccaatcaa agtattacaa acaaaacatg
    10021 tgaagactgg attgctgtca atgtcactcc ctcagtgatg tcttttcaac ttgaaggcat
    10081 gagtcccaga tgctttattg ccttccaggt tagggccttt acatctaagg ggccaggacc
    10141 atatgctgac gttgtaaagt ctacaacatc agaaatcaac ccatttcctc acctcataac
    10201 tcttcttggt aacaagatag tttttttaga tatggatcaa aatcaagttg tgtggacgtt
    10261 ttcagcagaa agagttatca gtgccgtttg ctacacagct gataatgaga tgggatatta
    10321 tgctgaaggg gactcactct ttcttctgca cttgcacaat cgctctagct ctgagctttt
    10381 ccaagattca ctggtttttg atatcacagt tattacaatt gactggattt caaggcacct
    10441 ctactttgca ctgaaagaat cacaaaatgg aatgcaagta tttgatgttg atcttgaaca
    10501 caaggtgaaa tatcccagag aggtgaagat tcacaatagg aattcaacaa taatttcttt
    10561 ttctgtatat cctcttttaa gtcgcttgta ttggacagaa gtttccaatt ttggctacca
    10621 gatgttctac tacagtatta tcagtcacac cttgcaccga attctgcaac ccacagctac
    10681 aaaccaacaa aacaaaagga atcaatgttc ttgtaatgtg actgaatttg agttaagtgg
    10741 agcaatggct attgatacct ctaacctaga gaaaccattg atatactttg ccaaagcaca
    10801 agagatctgg gcaatggatc tggaaggctg tcagtgttgg agagttatca cagtacctgc
    10861 tatgctcgca ggaaaaaccc ttgttagctt aactgtggat ggagatctta tatactggat
    10921 catcacagca aaggacagca cacagattta tcaggcaaag aaaggaaatg gggccatcgt
    10981 ttcccaggtg aaggccctaa ggagtaggca tatcttggct tacagttcag ttatgcagcc
    11041 ttttccagat aaagcgtttc tgtctctagc ttcagacact gtggaaccaa ctatacttaa
    11101 tgccactaac actagcctca caatcagatt acctctggcc aagacaaacc tcacatggta
    11161 tggcatcacc agccctactc caacatacct ggtttattat gcagaagtta atgacaggaa
    11221 aaacagctct gacttgaaat atagaattct ggaatttcag gacagtatag ctcttattga
    11281 agatttacaa ccattttcaa catacatgat acagatagct gtaaaaaatt attattcaga
    11341 tcctttggaa catttaccac caggaaaaga gatttgggga aaaactaaaa atggagtacc
    11401 agaggcagtg cagctcatta atacaactgt gcggtcagac accagcctca ttatatcttg
    11461 gagagaatct cacaagccaa atggacctaa agaatcagtc cgttatcagt tggcaatctc
    11521 acacctggcc ctaattcctg aaactcctct aagacaaagt gaatttccaa atggaaggct
    11581 cactctcctt gttactagac tgtctggtgg aaatatttat gtgttaaagg ttcttgcctg
    11641 ccactctgag gaaatgtggt gtacagagag tcatcctgtc actgtggaaa tgtttaacac
    11701 accagagaaa ccttattcct tggttccaga gaacactagt ttgcaattta attggaaggc
    11761 tccattgaat gttaacctca tcagattttg ggttgagcta cagaagtgga aatacaatga
    11821 gttttaccat gttaaaactt catgcagcca aggtcctgct tatgtctgta atatcacaaa
    11881 tctacaacct tatacttcat ataatgtcag agtagtggtg gtttataaga cgggagaaaa
    11941 tagcacctca cttccagaaa gctttaagac aaaagctgga gtcccaaata aaccaggcat
    12001 tcccaaatta ctagaaggga gtaaaaattc aatacagtgg gagaaagctg aagataatgg
    12061 atgtagaatt acatactata tccttgagat aagaaagagc acttcaaata atttacagaa
    12121 ccagaattta aggtggaaga tgacatttaa tggatcctgc agtagtgttt gcacatggaa
    12181 gtccaaaaac ctgaaaggaa tatttcagtt cagagtagta gctgcaaata atctagggtt
    12241 tggtgaatat agtggaatca gtgagaatat tatattagtt ggagatgatt tttggatacc
    12301 agaaacaagt ttcatactta ctattatagt tggaatattt ctggttgtta caatcccact
    12361 gacctttgtc tggcatagaa gattaaagaa tcaaaaaagt gccaaggaag gggtgacagt
    12421 gcttataaac gaagacaaag agttggctga gctgcgaggt ctggcagccg gagtaggcct
    12481 ggctaatgcc tgctatgcaa tacatactct tccaacccaa gaggagattg aaaatcttcc
    12541 tgccttccct cggg
//
