LOCUS       sequence-407876-       11089 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11089)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0322
FEATURES             Location/Qualifiers
     source          1..11089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6946..7534
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6999..7019)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7417..7428)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7606..7839
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7917..8038
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7954..7973)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8008..8027
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8078..8213
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8233..8252)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8234..8252)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8262..8279)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8262..8278)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8271..8293)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8420..8875
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8507..8526)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8717..8738
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8901..9005
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9006..9074,9075..9866)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9224..9243)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10037..10625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10526..10545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10779..10796
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10913..10934
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10949..10954,10955..10972,10973..10979)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10987..11003
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10992..11014
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11011..11027
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11011..11027
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11046..11066
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11048..11066
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggtg acattcaagg atgttgctgt
     4141 ggtcttcact gaggaggagc tggggctgct ggactctgtc cagaggaagc tgtaccgaga
     4201 tgtgatgctg gagaacttca ggaacctgct cttagtagca catcagccct tcaagccaga
     4261 cctaatatcc cagctggaga gagaagaaaa gcttttgatg gtggagacag aaaccccaag
     4321 agatggatgt tcaggaagga agaatcaaca aaagatggag agtattcagg aagtaacagt
     4381 aagctacttt tcccccaaag agctttcctc ccgtcagacc tggcaacaaa gtgcaggtgg
     4441 gttaatcagg tgtcaagatt tcctgaaagt ttttcaaggg aagaattctc agttgcaaga
     4501 acaaggtaat tccctcggcc aggtttgggc aggaatacca gttcagattt ctgaagataa
     4561 gaactatata ttcactcata tagggaatgg ctccaattat ataaaaagtc aagggtatcc
     4621 atcttggagg gcacatcatt cttggaggaa aatgtatctg aaagagtcac ataattatca
     4681 gtgtagatgt cagcaaattt ccatgaaaaa tcatttctgt aagtgtgaca gtgtcagttg
     4741 gctctcacat cacaatgata aactggaagt acacagaaaa gaaaactaca gctgccatga
     4801 ctgtggagaa gatatcatga aggtatcatt acttaatcag gagtcaattc aaacagagga
     4861 gaagccctat ccatgtactg ggtatagaaa agccttcagt aatgactcca gctctgaggt
     4921 tcatcagcag ttccacttgg aagggaagcc ctatacatac agttcatgtg gaaagggctg
     4981 taattatagt tcacttcttc atattcatca aaatattgag agagaagatg atattgagaa
     5041 ttcacatctg aaatcctatc agagagtgca tacagaggag aaaccatgca aatgtggtga
     5101 atatggtgag aacttcaatc actgttcccc tcttaacact tatgaactta tccacacagg
     5161 tgagatgtcc tataggcaca acatttatga gaaagccttc agtcatagct tagaccttaa
     5221 tagtattttt agggtccata ctagggatga accccatgaa tatgaggaaa atgagaatgt
     5281 ctttaatcag agttcatgtc ttcaagtcca tcaaaaaatc cacactgaag agaaactata
     5341 cacagatata gagtatggaa agagtttcat ttgtagttca aatcttgaca ttcagcatag
     5401 ggttcatatg gaagagaatt catataattc tgaggagtgt ggtaatggct tcagtctggc
     5461 ctcacatttt caggaccttc agatagtcca cactaaggaa caaccatata aacgctatgt
     5521 gtgtagtaac agcttcagcc ataatttata tcttcaaggt catccaaaaa ttcacattgg
     5581 agagaaacca cgtaaggagc atgggaatgg cttcaactgg agctcaaaac ttaaagatca
     5641 tcagagagtc cacactggac agaagccata caaatgcaat atatgcggca aaggtttcaa
     5701 tcatagatca gttctgaatg ttcatcagag agtccacaca ggagagaaac cttataaatg
     5761 cgaggaatgt gataagggat tcagtcggag ttcatatctt caagcccatc agagagtcca
     5821 cactggagaa aaaccttata aatgtgagga atgtgggaag gggttcagtc gaaattcata
     5881 ccttcaaggc catcagagag ttcacactgg agaaaaacca tacaagtgtg aggagtgtgg
     5941 gaagggcttc agtcggagtt cacaccttca aggccatcag agagtccaca ctggagaaaa
     6001 accattcaaa tgtgaggaat gtgggaaggg gttcagttgg agctttaatc ttcaaattca
     6061 tcagagggtt cacacaggag aaaaacccta taaatgtgaa gaatgtggta aaggcttcag
     6121 taaggcctca acacttttgg cccatcagag ggtccacacg ggagagaagc cataccaatg
     6181 tgatgagtgt ggtaagagtt tcagtcagag atcatacctt cagagtcatc agagtgtcca
     6241 ttctggagaa agaccatata tatgtgaggt atgtggaaag ggcttcagtc agagagcata
     6301 tcttcaaggt catcagagag tccacactag agtgaaaccg tataaatgtg agatgtgtgg
     6361 gaagggcttt agtcagagtt cgcgccttga agcacatcgg agggttcaca caggagggaa
     6421 accatacaaa tgtgaggtgt gtacaaaggg tttcagtgag agttcacgcc ttcaagcaca
     6481 ccaaagggtt catgtggaag ggagacccta taaatgtgaa cagtgtggta agggtttcag
     6541 tgggtattca agtcttcaag cccatcacag agtccacaca ggagagaaac catacaaatg
     6601 tgaggtatgt ggaaagggct tcagtcagag atcaaatctt caggctcacc agagagtcca
     6661 cacaggagag aaaccataca aatgtgatgc atgtggtaag ggtttccgtt ggagctcagg
     6721 tcttctcatt catcaaagag tccatagtag tgataaattc tataaaagcg aagactatgg
     6781 taaggactac ccttcatcag agaatctaca cagaaatgaa gattctgttt tgttttagta
     6841 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     6901 ggacgacttc ggaaggtcta ttgggagatc acgcgttaag tcgacaatca acctctggat
     6961 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7021 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7081 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7141 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7201 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7261 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7321 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     7381 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7441 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7501 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     7561 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     7621 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     7681 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7741 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7801 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     7861 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     7921 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7981 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8041 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8101 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8161 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     8221 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     8281 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     8341 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     8401 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     8461 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     8521 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     8581 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     8641 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     8701 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     8761 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     8821 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     8881 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8941 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     9001 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     9061 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     9121 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9181 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     9241 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     9301 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     9361 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9421 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9481 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9541 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9601 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9661 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9721 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     9781 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9841 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     9901 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9961 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    10021 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    10081 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    10141 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    10201 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    10261 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    10321 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    10381 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    10441 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    10501 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10561 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10621 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10681 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    10741 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    10801 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    10861 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    10921 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    10981 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    11041 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagc
//
