LOCUS       sequence-408127-        9555 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses a mutant PDGFRb gene (559-562 deletion)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9555)
  AUTHORS   Shima Y, Sasagawa S, Ota N, Oyama R, Tanaka M, Kubota-Sakashita M, 
            Kawakami H, Kobayashi M, Takubo N, Ozeki AN, Sun X, Kim YJ, Kamatani
            Y, Matsuda K, Maejima K, Fujita M, Noda K, Kamiyama H, Tanikawa R, 
            Nagane M, Shibahara J, Tanaka T, Rikitake Y, Mataga N, Takahashi S, 
            Kosaki K, Okano H, Furihata T, Nakaki R, Akimitsu N, Wada Y, Ohtsuka
            T, Kurihara H, Kamiguchi H, Okabe S, Nakafuku M, Kato T, Nakagawa H,
            Saito N, Nakatomi H
  TITLE     Increased PDGFRB and NF-kappaB signaling caused by highly prevalent 
            somatic mutations in intracranial aneurysms.
  JOURNAL   Sci Transl Med. 2023 Jun 14;15(700):eabq7721. doi: 
            10.1126/scitranslmed.abq7721. Epub 2023 Jun 14.
  PUBMED    37315111
REFERENCE   2  (bases 1 to 9555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2023-06-14"; volume: "15(700):eabq7721. doi"; pages: "
            10.1126/scitranslmed.abq7721. Epub 2023 Jun 14"
COMMENT     Sequence Label: pCAGGS-PDGFRb559-562del-IRES2-EGFP
FEATURES             Location/Qualifiers
     source          1..9555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..498
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        500..775
                     /label=chicken beta-actin promoter
     intron          776..1792
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1716..1738
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             3446..3592
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KEIR"
     misc_feature    join(5420..5994,5995..5997,5998..6006)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5587..5604)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5814..5833
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6007..6009,6010..6012,6013..6726)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6007..6009,6010..6012,6013..6723)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6052..6073)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6313..6332)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6660..6681
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6756..6811
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6810..6829)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7172..7188)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7172..7188)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7185..7207)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7196..7212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7220..7226,7227..7244,7245..7250))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7265..7286
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7345..7540
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7391..7526
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7546..7680
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7583..7602)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7637..7656
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7748..7765)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7919..8507)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7999..8018)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8678..9469,9470..9538))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9301..9320
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9539..9555,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
      121 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      181 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      241 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      301 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      361 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      421 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      481 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
      541 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
      601 ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg
      661 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
      721 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
      781 cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg
      841 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
      901 ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct
      961 tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg
     1021 tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg
     1081 gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg
     1141 tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
     1201 gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc
     1261 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc
     1321 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc
     1381 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
     1441 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
     1501 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1561 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
     1621 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
     1681 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
     1741 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     1801 caacgtgctg gttattggga gagggcagta aggaggactt cctggagggg gtgactgtcc
     1861 agagcctgga actgtgccca caccagaagc catcagcagc aaggacacca tgcggcttcc
     1921 gggtgcgatg ccagctctgg ccctcaaagg cgagctgctg ttgctgtctc tcctgttact
     1981 tctggaacca cagatctctc agggcctggt cgtcacaccc ccggggccag agcttgtcct
     2041 caatgtctcc agcaccttcg ttctgacctg ctcgggttca gctccggtgg tgtgggaacg
     2101 gatgtcccag gagcccccac aggaaatggc caaggcccag gatggcacct tctccagcgt
     2161 gctcacactg accaacctca ctgggctaga cacgggagaa tacttttgca cccacaatga
     2221 ctcccgtgga ctggagaccg atgagcggaa acggctctac atctttgtgc cagatcccac
     2281 cgtgggcttc ctccctaatg atgccgagga actattcatc tttctcacgg aaataactga
     2341 gatcaccatt ccatgccgag taacagaccc acagctggtg gtgacactgc acgagaagaa
     2401 aggggacgtt gcactgcctg tcccctatga tcaccaacgt ggcttttctg gtatctttga
     2461 ggacagaagc tacatctgca aaaccaccat tggggacagg gaggtggatt ctgatgccta
     2521 ctatgtctac agactccagg tgtcatccat caacgtctct gtgaacgcag tgcagactgt
     2581 ggtccgccag ggtgagaaca tcaccctcat gtgcattgtg atcgggaatg aggtggtcaa
     2641 cttcgagtgg acataccccc gcaaagaaag tgggcggctg gtggagccgg tgactgactt
     2701 cctcttggat atgccttacc acatccgctc catcctgcac atccccagtg ccgagttaga
     2761 agactcgggg acctacacct gcaatgtgac ggagagtgtg aatgaccatc aggatgaaaa
     2821 ggccatcaac atcaccgtgg ttgagagcgg ctacgtgcgg ctcctgggag aggtgggcac
     2881 actacaattt gctgagctgc atcggagccg gacactgcag gtagtgttcg aggcctaccc
     2941 accgcccact gtcctgtggt tcaaagacaa ccgcaccctg ggcgactcca gcgctggcga
     3001 aatcgccctg tccacgcgca acgtgtcgga gacccggtat gtgtcagagc tgacactggt
     3061 tcgcgtgaag gtggcagagg ctggccacta caccatgcgg gccttccatg aggatgctga
     3121 ggtccagctc tccttccagc tacagatcaa tgtccctgtc cgagtgctgg agctaagtga
     3181 gagccaccct gacagtgggg aacagacagt ccgctgtcgt ggccggggca tgccccagcc
     3241 gaacatcatc tggtctgcct gcagagacct caaaaggtgt ccacgtgagc tgccgcccac
     3301 gctgctgggg aacagttccg aagaggagag ccagctggag actaacgtga cgtactggga
     3361 ggaggagcag gagtttgagg tggtgagcac actgcgtctg cagcacgtgg atcggccact
     3421 gtcggtgcgc tgcacgctgc gcaacgctgt gggccaggac acgcaggagg tcatcgtggt
     3481 gccacactcc ttgcccttta aggtggtggt gatctcagcc atcctggccc tggtggtgct
     3541 caccatcatc tcccttatca tcctcatcat gctttggcag aaggagatcc gatggaaggt
     3601 gattgagtct gtgagctctg acggccatga gtacatctac gtggacccca tgcagctgcc
     3661 ctatgactcc acgtgggagc tgccgcggga ccagcttgtg ctgggacgca ccctcggctc
     3721 tggggccttt gggcaggtgg tggaggccac ggctcatggc ctgagccatt ctcaggccac
     3781 gatgaaagtg gccgtcaaga tgcttaaatc cacagcccgc agcagtgaga agcaagccct
     3841 tatgtcggag ctgaagatca tgagtcacct tgggccccac ctgaacgtgg tcaacctgtt
     3901 gggggcctgc accaaaggag gacccatcta tatcatcact gagtactgcc gctacggaga
     3961 cctggtggac tacctgcacc gcaacaaaca caccttcctg cagcaccact ccgacaagcg
     4021 ccgcccgccc agcgcggagc tctacagcaa tgctctgccc gttgggctcc ccctgcccag
     4081 ccatgtgtcc ttgaccgggg agagcgacgg tggctacatg gacatgagca aggacgagtc
     4141 ggtggactat gtgcccatgc tggacatgaa aggagacgtc aaatatgcag acatcgagtc
     4201 ctccaactac atggcccctt acgataacta cgttccctct gcccctgaga ggacctgccg
     4261 agcaactttg atcaacgagt ctccagtgct aagctacatg gacctcgtgg gcttcagcta
     4321 ccaggtggcc aatggcatgg agtttctggc ctccaagaac tgcgtccaca gagacctggc
     4381 ggctaggaac gtgctcatct gtgaaggcaa gctggtcaag atctgtgact ttggcctggc
     4441 tcgagacatc atgcgggact cgaattacat ctccaaaggc agcacctttt tgcctttaaa
     4501 gtggatggct ccggagagca tcttcaacag cctctacacc accctgagcg acgtgtggtc
     4561 cttcgggatc ctgctctggg agatcttcac cttgggtggc accccttacc cagagctgcc
     4621 catgaacgag cagttctaca atgccatcaa acggggttac cgcatggccc agcctgccca
     4681 tgcctccgac gagatctatg agatcatgca gaagtgctgg gaagagaagt ttgagattcg
     4741 gccccccttc tcccagctgg tgctgcttct cgagagactg ttgggcgaag gttacaaaaa
     4801 gaagtaccag caggtggatg aggagtttct gaggagtgac cacccagcca tccttcggtc
     4861 ccaggcccgc ttgcctgggt tccatggcct ccgatctccc ctggacacca gctccgtcct
     4921 ctatactgcc gtgcagccca atgagggtga caacgactat atcatccccc tgcctgaccc
     4981 caaacccgag gttgctgacg agggcccact ggagggttcc cccagcctag ccagctccac
     5041 cctgaatgaa gtcaacacct cctcaaccat ctcctgtgac agccccctgg agccccagga
     5101 cgaaccagag ccagagcccc agcttgagct ccaggtggag ccggagccag agctggaaca
     5161 gttgccggat tcggggtgcc ctgcgcctcg ggcggaagca gaggatagct tcctgtaggg
     5221 ggctggcccc taccctgccc tgcctgaagc tccccccctg ccagcaccca gcatctcctg
     5281 gcctggcctg accgggcttc ctgtcagcca ggctgccctt atcagctgtc cccttctgga
     5341 agctttctgc tcctgacgtg ttgtgcccca aaccctgggg ctggcttagg aggcaagaat
     5401 gatatcgaat tgggatccgc ccctctccct cccccccccc taacgttact ggccgaagcc
     5461 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     5521 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     5581 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     5641 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     5701 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     5761 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     5821 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     5881 ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     5941 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     6001 acaaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     6061 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     6121 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     6181 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     6241 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     6301 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     6361 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     6421 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     6481 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     6541 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     6601 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     6661 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     6721 aagtaaagcg gccgctgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     6781 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     6841 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
     6901 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     6961 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     7021 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     7081 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
     7141 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7201 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7261 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7321 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct
     7381 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7441 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     7501 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca
     7561 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7621 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     7681 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7741 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7801 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7861 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7921 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7981 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8041 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8101 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8161 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8221 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8281 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8341 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8401 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8461 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8521 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8581 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8641 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8701 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8761 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8821 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8881 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8941 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9001 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9061 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9121 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9181 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9241 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9301 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9361 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9421 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9481 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9541 tcttcctttt tcaat
//
