LOCUS       sequence-408440-        8719 bp    DNA     circular SYN 14-APR-2025
DEFINITION  In vitro transcription plasmid for production of dSpCas9-VPR mRNA 
            for transcriptional activation (VPR = tripartite transactivator 
            VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Jensen TI, Mikkelsen NS, Gao Z, Fosselteder J, Pabst G, Axelgaard E,
            Laustsen A, Konig S, Reinisch A, Bak RO
  TITLE     Targeted regulation of transcription in primary cells using CRISPRa 
            and CRISPRi.
  JOURNAL   Genome Res. 2021 Nov;31(11):2120-2130. doi: 10.1101/gr.275607.121. 
            Epub 2021 Aug 18.
  PUBMED    34407984
REFERENCE   2  (bases 1 to 8719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.275607.121"; journalName: "Genome Res"; date: "2021-11";
            volume: "31"; issue: "11"; pages: "2120-2130"
COMMENT     Sequence Label: dSpCas9-VPR
FEATURES             Location/Qualifiers
     source          1..8719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      475..484
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(481..507,508..510,511..2994,2995..3000,3001..3066,
                     3067..3069,3070..4584)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4597..4617
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    4654..4678
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4708..4857
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4882..4902
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5332..5688
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             5707..6276
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     primer_bind     complement(6498..6514)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6498..6514)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6511..6533)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6522..6538
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6546..6552,6553..6570,6571..6576))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6591..6612
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6729..6746)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6900..7488)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6980..6999)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7659..8450,8451..8519))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8282..8301
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8520..8624)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8692..8710
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctaatacg
      421 actcactata aggaaataag agagaaaaga agagtaagaa gaaatatagc tagcgccacc
      481 atggacaaga agtactccat tgggctcgct atcggcacaa acagcgtcgg ctgggccgtc
      541 attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc
      601 cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga gacggccgaa
      661 gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc
      721 tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg
      781 ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc
      841 aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag
      901 aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat
      961 atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat
     1021 gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga agagaacccg
     1081 atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc caaatcccgg
     1141 cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct gtttggtaat
     1201 cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga cctggccgaa
     1261 gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa tctgctggcc
     1321 cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc agacgccatt
     1381 ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct gagcgctagt
     1441 atgatcaagc gctatgatga gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga
     1501 cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc
     1561 ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa gcccatcttg
     1621 gaaaaaatgg acggcaccga ggagctgctg gtaaagctta acagagaaga tctgttgcgc
     1681 aaacagcgca ctttcgacaa tggaagcatc ccccaccaga ttcacctggg cgaactgcac
     1741 gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag ggaaaagatt
     1801 gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg gggaaattcc
     1861 agattcgcgt ggatgactcg caaatcagaa gagaccatca ctccctggaa cttcgaggaa
     1921 gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa
     1981 aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgagta cttcacagtt
     2041 tataacgagc tcaccaaggt caaatacgtc acagaaggga tgagaaagcc agcattcctg
     2101 tctggagagc agaagaaagc tatcgtggac ctcctcttca agacgaaccg gaaagttacc
     2161 gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc tgttgaaatc
     2221 agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct cctgaaaatc
     2281 attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga ggacattgtc
     2341 ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa aacttacgct
     2401 catctcttcg acgacaaagt catgaaacag ctcaagaggc gccgatatac aggatggggg
     2461 cggctgtcaa gaaaactgat caatgggatc cgagacaagc agagtggaaa gacaatcctg
     2521 gattttctta agtccgatgg atttgccaac cggaacttca tgcagttgat ccatgatgac
     2581 tctctcacct ttaaggagga catccagaaa gcacaagttt ctggccaggg ggacagtctt
     2641 cacgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat actgcagacc
     2701 gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga gaatatcgtt
     2761 atcgagatgg cccgagagaa ccaaactacc cagaagggac agaagaacag tagggaaagg
     2821 atgaagagga ttgaagaggg tataaaagaa ctggggtccc aaatccttaa ggaacaccca
     2881 gttgaaaaca cccagcttca gaatgagaag ctctacctgt actacctgca gaacggcagg
     2941 gacatgtacg tggatcagga actggacatc aatcggctct ccgactacga cgtggctgct
     3001 atcgtgcccc agtcttttct caaagatgat tctattgata ataaagtgtt gacaagatcc
     3061 gataaagcta gagggaagag tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa
     3121 aattattggc ggcagctgct gaacgccaaa ctgatcacac aacggaagtt cgataatctg
     3181 actaaggctg aacgaggtgg cctgtctgag ttggataaag ccggcttcat caaaaggcag
     3241 cttgttgaga cacgccagat caccaagcac gtggcccaaa ttctcgattc acgcatgaac
     3301 accaagtacg atgaaaatga caaactgatt cgagaggtga aagttattac tctgaagtct
     3361 aagctggtct cagatttcag aaaggacttt cagttttata aggtgagaga gatcaacaat
     3421 taccaccatg cgcatgatgc ctacctgaat gcagtggtag gcactgcact tatcaaaaaa
     3481 tatcccaagc ttgaatctga atttgtttac ggagactata aagtgtacga tgttaggaaa
     3541 atgatcgcaa agtctgagca ggaaataggc aaggccaccg ctaagtactt cttttacagc
     3601 aatattatga attttttcaa gaccgagatt acactggcca atggagagat tcggaagcga
     3661 ccacttatcg aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg tagggatttc
     3721 gcgacagtcc ggaaggtcct gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta
     3781 cagaccggag gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc
     3841 gcacgcaaaa aagattggga ccccaagaaa tacggcggat tcgattctcc tacagtcgct
     3901 tacagtgtac tggttgtggc caaagtggag aaagggaagt ctaaaaaact caaaagcgtc
     3961 aaggaactgc tgggcatcac aatcatggag cgatcaagct tcgaaaaaaa ccccatcgac
     4021 tttctcgagg cgaaaggata taaagaggtc aaaaaagacc tcatcattaa gcttcccaag
     4081 tactctctct ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc gggcgagctg
     4141 cagaaaggta acgagctggc actgccctct aaatacgtta atttcttgta tctggccagc
     4201 cactatgaaa agctcaaagg gtctcccgaa gataatgagc agaagcagct gttcgtggaa
     4261 caacacaaac actaccttga tgagatcatc gagcaaataa gcgaattctc caaaagagtg
     4321 atcctcgccg acgctaacct cgataaggtg ctttctgctt acaataagca cagggataag
     4381 cccatcaggg agcaggcaga aaacattatc cacttgttta ctctgaccaa cttgggcgcg
     4441 cctgcagcct tcaagtactt cgacaccacc atagacagaa agcggtacac ctctacaaag
     4501 gaggtcctgg acgccacact gattcatcag tcaattacgg ggctctatga aacaagaatc
     4561 gacctctctc agctcggtgg agacagcagg gctgacccca agaagaagag gaaggtgtcg
     4621 ccagggatcc gtcgacttga cgcgttgata tcaacaagtt tgtacaaaaa agcaggctac
     4681 aaagaggcca gcggttccgg acgggctgac gcattggacg attttgatct ggatatgctg
     4741 ggaagtgacg ccctcgatga ttttgacctt gacatgcttg gttcggatgc ccttgatgac
     4801 tttgacctcg acatgctcgg cagtgacgcc cttgatgatt tcgacctgga catgctgatt
     4861 aactctagaa gttccggatc tccgaaaaag aaacgcaaag ttggtagcca gtacctgccc
     4921 gacaccgacg accggcaccg gatcgaggaa aagcggaagc ggacctacga gacattcaag
     4981 agcatcatga agaagtcccc cttcagcggc cccaccgacc ctagacctcc acctagaaga
     5041 atcgccgtgc ccagcagatc cagcgccagc gtgccaaaac ctgcccccca gccttacccc
     5101 ttcaccagca gcctgagcac catcaactac gacgagttcc ctaccatggt gttccccagc
     5161 ggccagatct ctcaggcctc tgctctggct ccagcccctc ctcaggtgct gcctcaggct
     5221 cctgctcctg caccagctcc agccatggtg tctgcactgg ctcaggcacc agcacccgtg
     5281 cctgtgctgg ctcctggacc tccacaggct gtggctccac cagcccctaa acctacacag
     5341 gccggcgagg gcacactgtc tgaagctctg ctgcagctgc agttcgacga cgaggatctg
     5401 ggagccctgc tgggaaacag caccgatcct gccgtgttca ccgacctggc cagcgtggac
     5461 aacagcgagt tccagcagct gctgaaccag ggcatccctg tggcccctca caccaccgag
     5521 cccatgctga tggaataccc cgaggccatc acccggctcg tgacaggcgc tcagaggcct
     5581 cctgatccag ctcctgcccc tctgggagca ccaggcctgc ctaatggact gctgtctggc
     5641 gacgaggact tcagctctat cgccgatatg gatttctcag ccttgctggg ctctggcagc
     5701 ggcagccggg attccaggga agggatgttt ttgccgaagc ctgaggccgg ctccgctatt
     5761 agtgacgtgt ttgagggccg cgaggtgtgc cagccaaaac gaatccggcc atttcatcct
     5821 ccaggaagtc catgggccaa ccgcccactc cccgccagcc tcgcaccaac accaaccggt
     5881 ccagtacatg agccagtcgg gtcactgacc ccggcaccag tccctcagcc actggatcca
     5941 gcgcccgcag tgactcccga ggccagtcac ctgttggagg atcccgatga agagacgagc
     6001 caggctgtca aagcccttcg ggagatggcc gatactgtga ttccccagaa ggaagaggct
     6061 gcaatctgtg gccaaatgga cctttcccat ccgcccccaa ggggccatct ggatgagctg
     6121 acaaccacac ttgagtccat gaccgaggat ctgaacctgg actcacccct gaccccggaa
     6181 ttgaacgaga ttctggatac cttcctgaac gacgagtgcc tcttgcatgc catgcatatc
     6241 agcacaggac tgtccatctt cgacacatct ctgttttgag cggccgctta attaagctgc
     6301 cttctgcggg gcttgccttc tggccatgcc cttcttctct cccttgcacc tgtacctctt
     6361 ggtctttgaa taaagcctga gtaggaagtc taraaaaaaa aaaaaaaaaa aaaaaaaaaa
     6421 aaaaaaaaaa aaaaaaaaaa aaaaaagaag agcatcatcg cctgcagagg cctgcatgca
     6481 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6541 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6601 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6661 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6721 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6781 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6841 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6901 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6961 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7021 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7081 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7141 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7201 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7261 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7321 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7381 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7441 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7501 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7561 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7621 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7681 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7741 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7801 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7861 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7921 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7981 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8041 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8101 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     8161 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     8221 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     8281 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     8341 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     8401 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     8461 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8521 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8581 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8641 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     8701 atcacgaggc cctttcgtc
//
