LOCUS       sequence-408878-       11251 bp    DNA     circular SYN 14-APR-2025
DEFINITION  lentiviral plasmid for expression of mouse PD-L1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11251)
  AUTHORS   Pizzato HA, Alonso-Guallart P, Woods J, Connelly JP, Fehniger TA, 
            Atkinson JP, Pruett-Miller SM, Monsma FJ Jr, Bhattacharya D
  TITLE     Engineering human pluripotent stem cell lines to evade xenogeneic 
            transplantation barriers.
  JOURNAL   Stem Cell Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003.
  PUBMED    38215755
REFERENCE   2  (bases 1 to 11251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003."
COMMENT     Sequence Label: TRE-UBC-mPD-L1-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..11251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2610..2628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2646..2664
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2681..2699
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2717..2735
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2753..2771
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2788..2806
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2824..2842
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2854..2892
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2889..2913
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2988..4199
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4036..4055
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5106..5159
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5163..5165,5166..5879)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIKV
                     NFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5205..5226)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5466..5485)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5813..5834
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5939..6527
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5992..6012)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6410..6421)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6530..6546)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6531..6547)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7056..7236
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7262..7279)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7268..7492
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7538..7966
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7625..7644)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7835..7856
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7975..7995)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7980..8309
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8160..8295
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8222..8241
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8357..8404
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8423..8797
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8927..9048
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8964..8983)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9018..9037
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9097..9113)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9097..9113)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9110..9132)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9121..9137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9145..9151,9152..9169,9170..9175))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9190..9211
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9328..9345)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9499..10087)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9579..9598)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10258..11049,11050..11118))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10881..10900
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11119..11223)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagttttact ccctatcagt gatagagaac gtatgtcgag
     2641 tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa
     2701 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     2761 agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt
     2821 tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat
     2881 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattcgtta acggatcctc
     2941 tagagctcag cctcgagatt taaatttaat taacccaccc aagatctggc ctccgcgccg
     3001 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     3061 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     3121 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     3181 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     3241 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     3301 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     3361 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     3421 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     3481 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     3541 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     3601 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     3661 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     3721 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     3781 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     3841 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     3901 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     3961 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     4021 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     4081 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     4141 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
     4201 aagcttggta ccgagctcgg atctccaccc cgtacatgag gatatttgct ggcattatat
     4261 tcacagcctg ctgtcacttg ctacgggcgt ttactatcac ggctccaaag gacttgtacg
     4321 tggtggagta tggcagcaac gtcacgatgg agtgcagatt ccctgtagaa cgggagctgg
     4381 acctgcttgc gttagtggtg tactgggaaa aggaagatga gcaagtgatt cagtttgtgg
     4441 caggagagga ggaccttaag cctcagcaca gcaacttcag ggggagagcc tcgctgccaa
     4501 aggaccagct tttgaaggga aatgctgccc ttcagatcac agacgtcaag ctgcaggacg
     4561 caggcgttta ctgctgcata atcagctacg gtggtgcgga ctacaagcga atcacgctga
     4621 aagtcaatgc cccataccgc aaaatcaacc agagaatttc cgtggatcca gccacttctg
     4681 agcatgaact aatatgtcag gccgagggtt atccagaagc tgaggtaatc tggacaaaca
     4741 gtgaccacca acccgtgagt gggaagagaa gtgtcaccac ttcccggaca gaggggatgc
     4801 ttctcaatgt gaccagcagt ctgagggtca acgccacagc gaatgatgtt ttctactgta
     4861 cgttttggag atcacagcca gggcaaaacc acacagcgga gctgatcatc ccagaactgc
     4921 ctgcaacaca tcctccacag aacaggactc actgggtgct tctgggatcc atcctgttgt
     4981 tcctcattgt agtgtccacg gtcctcctct tcttgagaaa acaagtgaga atgctagatg
     5041 tggagaaatg tggcgttgaa gatacaagct caaaaaaccg aaatgataca caattcgagg
     5101 agacggaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggccctg
     5161 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     5221 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     5281 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     5341 tcgtgaccac cttgacctac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc
     5401 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     5461 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     5521 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     5581 agctggagta caactacaac agccacaagg tctatatcac cgccgacaag cagaagaacg
     5641 gcatcaaggt gaacttcaag acccgccaca acatcgagga cggcagcgtg cagctcgccg
     5701 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     5761 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     5821 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat
     5881 gtacaagtaa agcggccgcg actctagtcc ctcccaattc gatatcaagc ttatcgataa
     5941 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6001 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6061 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6121 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6181 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6241 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6301 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6361 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6421 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6481 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     6541 acctcgatcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     6601 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     6661 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6721 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     6781 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     6841 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     6901 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     6961 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     7021 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     7081 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7141 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7201 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     7261 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     7321 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     7381 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     7441 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     7501 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     7561 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7621 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7681 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7741 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7801 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7861 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7921 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     7981 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     8041 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     8101 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     8161 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8221 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     8281 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     8341 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     8401 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     8461 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     8521 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     8581 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     8641 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     8701 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     8761 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     8821 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     8881 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     8941 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9001 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     9061 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     9121 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     9181 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9241 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9301 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9361 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9421 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9481 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9541 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9601 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9661 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9721 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9781 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9841 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9901 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9961 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10021 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10081 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10141 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    10201 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10261 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10321 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10381 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10441 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10501 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10561 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10621 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10681 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10741 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10801 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10861 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10921 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10981 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    11041 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    11101 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    11161 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    11221 gcgcacattt ccccgaaaag tgccacctga c
//
