LOCUS       sequence-409148-        9214 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0793
FEATURES             Location/Qualifiers
     source          1..9214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2152..2740
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2205..2225)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2623..2634)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2812..3045
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3123..3244
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3160..3179)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3214..3233
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3284..3419
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3439..3458)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3440..3458)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3468..3485)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3468..3484)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3477..3499)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3626..4081
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3713..3732)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3923..3944
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4107..4211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4212..4280,4281..5072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4430..4449)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5243..5831
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5732..5751
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5985..6002
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6119..6140
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6155..6160,6161..6178,6179..6185)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6193..6209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6198..6220
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6217..6233
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6217..6233
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6252..6272
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6254..6272
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6300..6526
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6527..6707
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6754..6879
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7372..7605
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7790..7834
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8065..8275
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8087..8283
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8134..8269
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8315..8917
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8315..8334)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8811..8831
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8960..9077
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtc
     1201 actcctggcg accctggggc tggagctgga cagggccctg ctcccagcta gtgggctggg
     1261 atggctcgta gactatggga aactcccccc ggcccctgcc cccctggctc cctatgaggt
     1321 ccttggggga gccctggagg gcgggcttcc agtgggggga gagcccctgg caggtgatgg
     1381 cttctctgac tggatgactg agcgagttga tttcacagct ctcctccctc tggagcctcc
     1441 cttacccccc ggcaccctcc cccaaccttc cccaaccccm cctgacctgg aagctatggc
     1501 ctccctcctc aagaaggagc tggaacagat ggaagacttc ttcctagats ccccgccccy
     1561 cccmcccccc cccccgccgc cactaccacc accaccacta ccaccagccc cctccctccc
     1621 cctgtccctc ccctcctttg acctccccca gccccctgtc ttggatactc tggacttgct
     1681 ggccatctac tgccgcaacg aggccgggca ggaggaagtg gggatgccgc ctctgccccc
     1741 gccacagcag ccccctcctc cttctccacc tcaaccttct cgcctggccc cctacccaca
     1801 tcctgccacc acccgagggg accgcaagca aaagaagaga gaccagaaca agtcggcggc
     1861 tctgaggtac cgccagcgga agcgggcaga gggtgaggcc ctggagggcg agtgccaggg
     1921 gctggaggca cggaatcgcg agctgaagga acgggcagag tccgtggagc gcgagatcca
     1981 gtacgtcaag gacctgctca tcgaggttta caaggcccgg agccagagga cccgtagctg
     2041 ctagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     2101 tggaaaggac gagcatggga agtggacacg tacgcaacgc gttaagtcga caatcaacct
     2161 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     2221 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     2281 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     2341 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     2401 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     2461 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     2521 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     2581 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     2641 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     2701 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     2761 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     2821 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     2881 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     2941 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     3001 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     3061 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     3121 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3181 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3241 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     3301 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3361 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3421 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     3481 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     3541 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     3601 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3661 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3721 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3781 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3841 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3901 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3961 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4021 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     4081 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4141 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4201 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4261 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4321 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4381 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4441 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4501 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4561 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4621 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4681 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4741 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4801 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4861 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     4921 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     4981 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5041 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5101 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5161 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5221 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5281 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5341 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5401 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5461 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5521 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5581 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5641 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5701 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5761 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5821 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5881 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5941 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6001 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6061 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6121 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6181 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     6241 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     6301 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     6361 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     6421 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     6481 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     6541 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6601 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6661 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     6721 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     6781 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     6841 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     6901 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     6961 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     7021 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     7081 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     7141 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     7201 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     7261 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     7321 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     7381 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     7441 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     7501 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     7561 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     7621 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     7681 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     7741 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     7801 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     7861 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     7921 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     7981 gacccacctc ccaaccccga ggggacccat gcattgcatc tcaattagtc agcaaccagg
     8041 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgcg tctcaattag
     8101 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8161 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8221 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     8281 aaaaagcttt ctagaggtac caccggagct taccatgacc gagtacaagc ccacggtgcg
     8341 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     8401 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     8461 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     8521 cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     8581 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     8641 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     8701 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     8761 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     8821 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     8881 cacctggtgc atgacccgca agcccggtgc cagttaacga attctagatc ttgagacaaa
     8941 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     9001 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     9061 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
     9121 tcgaggcctg caggtgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
     9181 atggaccttc taggtcttga aaggagtggg aatt
//
