LOCUS       sequence-409196-       14153 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Cadherin circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14153)
  AUTHORS   Wauford N, Patel A, Tordoff J, Enghuus C, Jin A, Toppen J, Kemp ML, 
            Weiss R
  TITLE     Synthetic symmetry breaking and programmable multicellular structure
            formation.
  JOURNAL   Cell Syst. 2023 Sep 20;14(9):806-818.e5. doi: 
            10.1016/j.cels.2023.08.001. Epub 2023 Sep 8.
  PUBMED    37689062
REFERENCE   2  (bases 1 to 14153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2023.08.001"; journalName: "Cell Syst"; date: 
            "2023-09-20- 20"; volume: "14"; issue: "9"; pages: "806-818.e5"
COMMENT     Sequence Label: aCE121
FEATURES             Location/Qualifiers
     source          1..14153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(20..38)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(201..220)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             212..1021
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(279..298)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1176..1764
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1665..1684
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1892..1912
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1930..1967
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    1977..2001
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2017..2616
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2017..2036)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2513..2533
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(2618..2642)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2732..2751)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2797..2852
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2851..2870)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        3826..4037
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          4038..4976
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4933..4953
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5117..5126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5123..5125,5126..5128,5129..5842)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     polyA_signal    5895..5943
                     /note="synthetic polyadenylation signal"
     CDS             9029..9085
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9086..9778
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGH"
     polyA_signal    9843..9891
                     /note="synthetic polyadenylation signal"
     protein_bind    10532..10565
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     regulatory      10576..10585
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             13243..13299
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(13300..13302,13303..13305,13306..14019)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    14072..14120
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gttcgcaatt caggaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
       61 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgctggc ccgtgtctca
      121 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
      181 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
      241 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
      301 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
      361 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
      421 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
      481 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
      541 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
      601 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
      661 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
      721 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
      781 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
      841 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
      901 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
      961 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     1021 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     1081 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1141 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1201 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1261 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1321 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1381 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1441 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1501 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1561 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1621 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1681 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1741 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1801 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggcatgc
     1861 gtatcgatgt cgacgttaac gctagtgata tcaactttgt atagaaaagt tggctccgaa
     1921 tttctcgagg gcttgtcgac gacggcggtc tccgtcgtca ggatcatgaa ttcgacccaa
     1981 gtttgtacaa aaaagcaggc tgaacggcct gccaccatga ccgagtacaa gcccacggtg
     2041 cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc
     2101 gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag
     2161 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     2221 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     2281 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     2341 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     2401 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     2461 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     2521 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     2581 cgcacctggt gcatgacccg caagcccggt gcctgatacc cagctttctt gtacaaagtg
     2641 gtacgcgtga attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg
     2701 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg
     2761 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
     2821 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
     2881 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
     2941 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
     3001 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
     3061 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
     3121 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc
     3181 tcgacgagga agcagagcgt aaaacgacgg ccaggcagta ccggagttct gcaattcatg
     3241 tgatctgcgc ctgggagctc acggggacag ccccccccca aagcccccag ggatgtaatt
     3301 acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg
     3361 ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc
     3421 acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg
     3481 gatacgggga aaaagctagg ctgaaagagg atcttagaat gacacgcgcc tgggagctca
     3541 cggggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg
     3601 gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc
     3661 ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa
     3721 cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa aaagctggat
     3781 cataactcga cgtgcatact ttaattggct ccggtgcccg tcagtgggca gagcgcacat
     3841 cgcccacagt ccccgagaag ttgtggggag gggtcggcaa ttgaaccggt gcctagagaa
     3901 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     3961 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     4021 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     4081 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     4141 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     4201 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     4261 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     4321 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     4381 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     4441 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     4501 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     4561 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     4621 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     4681 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     4741 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     4801 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     4861 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     4921 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgg
     4981 atcccaaggg cagatagttt taaagttggt tattagttac tgtgatattt atcacggtac
     5041 ccaataacca atgaaggagt agtgccccaa ctggggtaac ctttgagttc tctcagttgg
     5101 gggcgtaggg tcaggtgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     5161 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgaggggcga
     5221 gggcgagggc gatgccacca acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     5281 gctgcccgtg ccctggccca ccctcgtgac caccctgagc cacggcgtgc agtgcttcgc
     5341 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     5401 cgtccaggag cgcaccatct tcttcaagga cgacggcacc tacaagaccc gcgccgaggt
     5461 gaagttcgag ggcgacaccc tagtgaaccg catcgagctg aagggcgtcg acttcaagga
     5521 ggacggcaac atcctggggc acaagctgga gtacaacttc aacagccaca acatctatat
     5581 catggccgtc aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacgtgga
     5641 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     5701 cgtgctgctg cccgacagcc actacctgag cacccagtcc gtgctgagca aagaccccaa
     5761 cgagaagcgc gatcacatgg tcctgctgga gttccgcacc gccgccggga tcactctcgg
     5821 catggacgag ctgtacaagt gagcttccac tggattgtac aattaccaac tgatctagaa
     5881 ccggtcatgg ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt
     5941 gtgttcgaac tagatgctgt cgaccgatgc gctcgcggag ttctgcaatt catgtgatct
     6001 gcgcctggga gctcacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc
     6061 ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc
     6121 ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga
     6181 tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg
     6241 gggaaaaagc taggctgaaa gaggatctta gaatgacacg cgcctgggag ctcacgggga
     6301 cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca
     6361 gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc
     6421 gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc
     6481 tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagct ggatcataac
     6541 tcgacgtgca tacttaagtt tgtacaaaaa agagcagaaa ggtagcgtca acgataggtg
     6601 taactgtcgt gtttgtaacg gtacttccaa cagctggcga ggtgccacca tgagaactta
     6661 ccggtacttc ttgctgctct tttgggtcgg ccagccctac ccaactttct caaacccatt
     6721 atctaaaagg actagtggct tcccagcaaa gaggaaagcc ctggagctct ctgcaaacag
     6781 caggaatgag ctgagccgtt cgaaaaggag ttggatgtgg aatcagttct tcctcctgga
     6841 ggaatacacg ggatccgatt atcagtacgt gggcaagtta cattcagacc aggatagagg
     6901 agatggatca cttaaatata tcctttcagg agatggagcg ggcgatctct tcattatcaa
     6961 tgagaacaca ggcgacatac aggccaccaa gaggctggac agggaagaaa aacctgttta
     7021 catccttcga gctcaagctg tcaacagaag gacaggtaga cccgtcgagc ctgagtctga
     7081 attcatcatc aagatccacg acatcaatga caatgagcca atattcacca aggacgttta
     7141 cacagccaca gtcccggaga tggctgatgt tggcacattt gtggtccaag tcacggcgac
     7201 tgatgctgac gacccaacat atgggaacag tgctaaagtc gtctatagca tcctgcaggg
     7261 acagccctac ttttcagtgg aatcggaaac aggtatcatc aagacagcgt tgctcaacat
     7321 ggatcgagaa aacagagaac agtaccaagt ggtgattcag gccaaggaca tgggcggcca
     7381 gatgggagga ctgtcgggga ctacgaccgt gaacatcacg ctgacagatg tcaatgacaa
     7441 cccaccccgt ttcccccaga gtacctacca gtttaagacc cccgagtcct ctccaccggg
     7501 aacgccaatt ggcaggatca aagccagtga tgccgatgtg ggagaaaatg cggagatcga
     7561 gtacagcatc actgacggtg agggacacga gatgtttgat gtcatcaccg accaggaaac
     7621 ccaggaaggg atcataactg tcaaaaagct cttggatttt gagaagaaga aggtgtacac
     7681 cctcaaggtg gaagcctcca atccccacgt cgaaccccga tttctctacc tgggtccctt
     7741 caaagactcg gccacggtga gaatcgtggt ggacgatgtg gacgaacctc ctgtgttcag
     7801 caaactggcc tacatcctac agatacggga ggatgcccgg ataaatacga ccataggctc
     7861 tgtcgcagct caggatcctg acgctgccag gaatcctgtc aagtattctg tggatagaca
     7921 cacagatatg gacaggatat tcaacattga ttctggaaat ggttcgattt ttacatcaaa
     7981 acttctcgac cgggaaaccc tgctgtggca caacatcaca gtgatcgcaa cggagattaa
     8041 taacccaaag caaagcagcc gcgttcctct atatattaaa gttctcgacg tcaacgacaa
     8101 tgccccagag tttgctgagt tctatgaaac ttttgtctgt gaaaaagcaa aggcagatca
     8161 gctgattcag accctgcgtg ctgttgacaa ggatgatcct tacagcggac accaattttc
     8221 cttctccttg gccccagaag cagccagcag ctcaaacttt actatacaag acaacaaaga
     8281 caacactgca gggatcctaa ctcggaaaaa tggctataac agacacgaga tgagcaccta
     8341 tctcctgcct gtggtcattt cggacaacga ctacccagtc caaagcagca ctgggacagt
     8401 gactgtccgg gtctgtgcat gtgaccacca tgggaacatg cagtcctgcc acgccgaggc
     8461 cctcatccac cccacagggc tgagcacagg ggctctggtg gccatccttc tgtgcatcgt
     8521 gatcctacta gtgacagtgg tgctgttcgc agctctgagg cggcagcgaa agaaagagcc
     8581 tttgatcatt tccaaagagg acatcagaga taacattgtc agttacaacg acgaaggtgg
     8641 cggagaggag gacacccagg cctttgatat cggaaccctg aggaaccctg aagccatgga
     8701 ggacagcaaa tcgcgcaggg acattgtgcc tgaagctctt tttctacccc ggcggactcc
     8761 aacagctcgt gacaacacgg atgtcagaga tttcattaac caaaggttaa aggaaaatga
     8821 cacggaccca acagcccctc cttacgactc cttggccacc tatgcctacg aaggcaccgg
     8881 ctctgtggcc gactccctga gctcgctgga atccgtgacc acggatggag atcaagatta
     8941 tgactatttg agtgactggg gccctcggtt caaaaagctg gcagatatgt atggagggat
     9001 ggacagtgac aaagactccg gatctggcgc caccaacttc tctctgctga agcaggccgg
     9061 cgacgtggag gagaacccag gcccaatggt gagcgagctg attaaggaga acatgcacat
     9121 gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga
     9181 aggcaagccc tacgagggca cccagaccat gagaatcaag gcggtcgagg gcggccctct
     9241 ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcaaaa ccttcatcaa
     9301 ccacacccag ggcatccccg acttctttaa gcagtccttc cccgagggct tcacatggga
     9361 gagagtcacc acatacgaag atgggggcgt gctgaccgct acccaggaca ccagcctcca
     9421 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcccat ccaacggccc
     9481 tgtgatgcag aagaaaacac tcggctggga ggcctccacc gagacactgt accccgctga
     9541 cggcggcctg gaaggcagag ccgacatggc cctgaagctc gtgggcgggg gccacctgat
     9601 ctgcaacctt aagaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg
     9661 cgtctactat gtggacagga gactggaaag aatcaaggag gccgacaaag agacatacgt
     9721 cgagcagcac gaggtggctg tggccagata ctgcgacctc cctagcaaac tggggcacaa
     9781 acttaattga gcttccactg gattgtacaa ttaccaactg atctagaacc ggtcatggcc
     9841 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gttcgaacta
     9901 gatgctgtcg accgatgcgc ttacggagtt ctgcaattca tgtgatctgc gcctgggagc
     9961 tcacggggac agcccccccc caaagccccc agggatgtaa ttacgtccct cccccgctag
    10021 ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc gcatccccga
    10081 gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc gctttcctct
    10141 gaacgcttct cgctgctctt tgagcctgca gacacctggg gggatacggg gaaaaagcta
    10201 ggctgaaaga ggatcttaga atgacacgcg cctgggagct cacggggaca gccccccccc
    10261 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
    10321 gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag
    10381 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
    10441 gagcctgcag acacctgggg ggatacgggg aaaaagctgg atcataactc gacgtgcata
    10501 cttaagtttg tacaaaaaag agcagatgcg ggtgccaggg cgtgcccttg ggctccccgg
    10561 gcgcgtactc caggtgccac catgggagcc cggtgccgca gcttttccgc gctcctgctc
    10621 ctgctgcagg tatcctcatg gctttgccag gagctggagc ctgagtcctg cagtcccggc
    10681 ttcagttccg aggtctacac cttcccggtg ccggagaggc acctggagag aggccatgtc
    10741 ctgggcagag tgagatttga aggatgcact ggccggccaa ggacagcctt cttttcggag
    10801 gactcccgat tcaaagtggc gacagacggc accatcacag tgaagcggca tctaaagctc
    10861 cacaagctgg agactagttt cctcgtccgc gcccgggact ccagtcatag ggagctgtct
    10921 accaaagtga cgctgaagtc catggggcac caccatcacc ggcaccacca ccgcgaccct
    10981 gcctctgaat ccaacccaga gctgctcatg tttcccagcg tgtacccagg tctaagaagg
    11041 cagaaacgag actgggtcat ccctcccatc agctgccccg aaaatgaaaa gggcgaattt
    11101 ccaaagaacc tggttcagat caaatccaac agggacaaag aaacaaaggt tttctacagc
    11161 atcaccggcc aaggagctga caaacccccc gttggcgttt tcatcattga gagggagaca
    11221 ggctggctga aagtgacaca gcctctggat agagaagcca ttgccaagta catcctctat
    11281 tctcatgccg tgtcatcaaa tggggaagcg gtggaggatc ccatggagat agtgatcaca
    11341 gtgacagatc agaatgacaa caggccagag tttacccagc cggtctttga gggattcgtt
    11401 gcagaaggcg ctgttccagg aacctccgtg atgaaggtat cagccaccga tgcagacgat
    11461 gacgtcaaca cctacaacgc tgccatcgcc tacaccatcg tcagccagga tcctgagctg
    11521 cctcacaaaa acatgttcac tgtcaatagg gacaccgggg tcatcagtgt gctcacctct
    11581 gggctggacc gagagagtta ccctacatac actctggtgg ttcaggctgc tgaccttcaa
    11641 ggtgaaggct tgagcacaac agccaaggct gtgatcactg tcaaggatat taatgacaac
    11701 gctcctgtgt tcaacccaag cacgtatcag ggtcaagtgc ctgagaatga ggtcaatgcc
    11761 cggatcgcca cactcaaagt gaccgatgat gatgccccca acactccggc gtggaaagct
    11821 gtgtacaccg tagtcaacga tcctgaccag cagttcgttg ttgtcacaga ccccacgacc
    11881 aatgatggca ttttgaaaac agccaagggc ttggattttg aggccaagca gcaatacatc
    11941 cttcatgtga gagtggagaa cgaggaaccc tttgaggggt cacttgtccc ttccacagcc
    12001 actgtcactg tggacgtggt agacgtgaat gaagccccca tctttatgcc tgcggagagg
    12061 agagtcgaag tgcccgagga ctttggtgtg ggtcaggaaa tcacatctta taccgctcga
    12121 gagccggaca cgttcatgga tcagaagatc acgtatcgga tttggaggga cactgccaac
    12181 tggctggaga ttaacccaga gactggtgcc attttcacgc gcgctgagat ggacagagaa
    12241 gatgctgagc atgtgaagaa cagcacatat gtagctctca tcatcgccac agatgatggt
    12301 tcacccattg ccactggcac gggcactctt ctcctggtcc tgttagacgt caatgacaac
    12361 gctcccatcc cagaacctcg aaacatgcag ttctgccaga ggaacccaca gcctcatatc
    12421 atcaccatct tggatccaga ccttcccccc aacacgtccc cctttactgc tgagctaacc
    12481 catggggcca gcgtcaactg gaccattgag tataatgacg cagctcaaga atctctcatt
    12541 ttgcaaccaa gaaaggactt agagattggc gaatacaaaa tccatctcaa gctcgcggat
    12601 aaccagaaca aagaccaggt gaccacgttg gacgtccatg tgtgtgactg tgaagggacg
    12661 gtcaacaact gcatgaaggc gggaatcgtg gcagcaggat tgcaagttcc tgccatcctc
    12721 ggaatccttg gagggatcct cgccctgctg attctgatcc tgctgctcct actgtttcta
    12781 cggaggagaa cggtggtcaa agagcccctg ctgccaccag atgatgatac ccgggacaat
    12841 gtgtattact atgatgaaga aggaggtgga gaagaggacc aggactttga tttgagccag
    12901 ctgcacaggg gcctggatgc ccgaccggaa gtgactcgaa atgatgtggc tcccaccctc
    12961 atgagcgtgc cccagtatcg tccccgtcct gccaatcctg atgaaattgg aaacttcatc
    13021 gatgaaaacc tgaaggcagc cgacagcgac cccacggcac ccccttacga ctctctgttg
    13081 gtgttcgatt acgagggcag tggttctgaa gccgctagcc tgagctcact gaactcctct
    13141 gagtcggatc aggaccagga ctacgattat ctgaacgagt ggggcaaccg attcaagaag
    13201 ctggcggaca tgtacggcgg tggtgaggac gacggatctg gcgccaccaa cttctctctg
    13261 ctgaagcagg ccggcgacgt ggaggagaac ccaggcccaa tggtgagcaa gggcgaggag
    13321 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
    13381 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
    13441 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac
    13501 ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
    13561 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
    13621 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
    13681 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
    13741 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
    13801 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
    13861 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaag
    13921 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
    13981 gccgggatca ctctcggcat ggacgagctg tacaagtgag cttccactgg attgtacaat
    14041 taccaactga tctagaaccg gtcatggccg caataaaata tctttatttt cattacatct
    14101 gtgtgttggt tttttgtgtg ttcgaactag atgctgtcga ccgatgcgct aga
//
