LOCUS       sequence-409517-        2847 bp    DNA     circular SYN 15-APR-2025
DEFINITION  D12: Promoter of TraesCS4D02G131100 as Golden Gate compatible MoClo 
            PROM part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2847)
  TITLE     Schwessinger Lab Plasmids 2023
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PROM_12
FEATURES             Location/Qualifiers
     source          1..2847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(891..907)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(891..907)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(904..926)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1079..1667)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1159..1178)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1800..1894)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1915..2574)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2489..2508
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2575..2677)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2764..2786
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2778..2795
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2779..2795
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctctgtggtc tcaggaggcg ctccggtgcg tatccttcct aaaggcattg cgttggagtc
       61 cacggttcgt catatgcggc ttcatctctt cgcggtggcg tgttcacggt cgaggacccg
      121 gctgctcttg tagtgcttgg ggtggtgttg ctgcgctcag cgcttatgta tcttgctttg
      181 ggtgtgtgcg tgtgttgtga tggcgtgagt ttgtattggg ttgttgttgg tcgatgcttt
      241 atatataaaa tgggacgaaa ggctttttcg ctatgccata ttccgtgcac atctcaccca
      301 gcgcgtcggc gccgtcgact gctgtttcac atcgcggcgg tgctggccgg ccgttgacgt
      361 ccgcgttgcc gagcttcatt attcgccatc tcatatccat taatccacgt gctgtcattg
      421 tttcacgttg tatgggagca tttgaccctt ggacataaaa catgatatcg tggtgcttaa
      481 ttaattgact caaggaatag aggtcgacgc atgcaccata ctacctccat gatcgatgac
      541 aaacttctgc aagggacctg tacgcgtaca aaacacggag gatccaaatg tgtgtgctgc
      601 accaaaatga cgcggaaact aaggcgtcgt tgctgacaca tttctccatc gctgccaaca
      661 tgttgtcttg tgttcgcacg ccgcgtatat atacacccac cacgcgcgcc atattatccg
      721 tcaaccgtat caaagttttt gtaacgacac aaatcaagtt gctagctccc atttcagctg
      781 gtgatacctg caaatttggt gataaggtag ctaggtgaat gcgagaccac gaagaggcta
      841 ggtggaggct cagtgatgat aagtctgcga tggtggatgc atgtgtcatg gtcatagctg
      901 tttcctgtgt gaaattgtta tccgctcaga gggcacaatc ctattccgcg ctatccgaca
      961 atctccaaga cattaggtgg agttcagttc ggcgtatggc atatgtcgct ggaaagaaca
     1021 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1081 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1141 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1201 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1261 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1321 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1381 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1441 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1501 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1561 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1621 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1681 tcttttctac ggggtctgac gctctattca acaaagccgc cgtcccgtca agtcagcgta
     1741 aatgggtagg gggcttcaaa tcgtcccccc atacgatata agttgttact agtgcttgga
     1801 ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     1861 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
     1921 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     1981 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     2041 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     2101 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     2161 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     2221 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     2281 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     2341 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     2401 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     2461 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     2521 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     2581 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     2641 attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattgat acctgaaaca
     2701 aaacccatcg tacggccaag gaagtctcca ataactgtga tccaccacaa gcgccagggt
     2761 tttcccagtc acgacgttgt aaaacgacgg ccagtcatgc ataatccgca cgcatctgga
     2821 ataaggaagt gccattccgc ctgacct
//
