LOCUS       sequence-409793-        1149 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  pGL3 promoter luciferase reporter vector containing 3 copies of the 
            NBRE DNA response element (5′-GAGTTTTAAAAGGTCATGCTCAATT TGTC-3′) 
            used for luciferase reporter assays in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Munoz-Tello P, Lin H, Khan P, de Vera IMS, Kamenecka TM, Kojetin DJ
  TITLE     Assessment of NR4A Ligands That Directly Bind and Modulate the 
            Orphan Nuclear Receptor Nurr1.
  JOURNAL   J Med Chem. 2020 Dec 24;63(24):15639-15654. doi: 
            10.1021/acs.jmedchem.0c00894. Epub 2020 Dec 8.
  PUBMED    33289551
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.0c00894"; journalName: "J Med Chem"; date: 
            "2020-12-24- 24"; volume: "63"; issue: "24"; pages: "15639-15654"
COMMENT     Sequence Label: pGL3-NBRE3
FEATURES             Location/Qualifiers
     source          1..1149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        151..347
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      198..333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      377..386
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(449..467)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gnnnnnanat ttctctatcg ataggtaccg agctcttacg cgtgctagga gttttaaaag
       61 gtcatgctca atttgtcgag ttttaaaagg tcatgctcaa tttgtcgagt tttaaaaggt
      121 catgctcaat ttgtctcgag atctgcgatc tgcatctcaa ttagtcagca accatagtcc
      181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      241 atcgctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
      301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggcattc
      361 cggtactgtt ggtaaagcca ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc
      421 attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata
      481 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta
      541 cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa
      601 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt
      661 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga
      721 attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt
      781 gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga
      841 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc
      901 tcccggtttt aatgaatacg atttngtgcc agagtccttc gatagggana nanantgcac
      961 tgatcatgan nncnntggnt ctactggnct gnctaaaggn ntcnntctgc ncatannact
     1021 gcnnnnngag attctcgcat gcaganatcn atttttggca ncaatcntnn nanacngcnn
     1081 ttnnnnngtc nntcatnnnn nnnnngntnc nnnnnnncga nnttnannng nngantnnnn
     1141 nncnncnnt
//
