LOCUS       sequence-410234-        3405 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  expresses 
            PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP 
            component in rat hippocampal neuron cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Cui M, Lee S, Ban SH, Ryu JR, Shen M, Yang SH, Kim JY, Choi SK, Han 
            J, Kim Y, Han K, Lee D, Sun W, Kwon HB, Lee D
  TITLE     A single-component, light-assisted uncaging switch for 
            endoproteolytic release.
  JOURNAL   Nat Chem Biol. 2024 Mar;20(3):353-364. doi: 
            10.1038/s41589-023-01480-6. Epub 2023 Nov 16.
  PUBMED    37973890
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-023-01480-6"; journalName: "Nat Chem Biol"; date: 
            "2024-03"; volume: "20"; issue: "3"; pages: "353-364"
COMMENT     Sequence Label: 
            hSyn-PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FK
            BP
FEATURES             Location/Qualifiers
     source          1..3405
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..63
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     CDS             64..93
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             97..243
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             274..723
                     /codon_start=1
                     /product="first 150 residues of the mouse kainate receptor 
                     subunit 2"
                     /label=KA2
                     /note="mediates targeting to the neuronal soma (Shemesh et 
                     al., 2017)"
                     /translation="MPAELLLLLIVAFANPSCQVLSSLRMAAILDDQTVCGRGERLALA
                     LAREQINGIIEVPAKARVEVDIFELQRDSQYETTDTMCQILPKGVVSVLGPSSSPASAS
                     TVSHICGEKEIPHIKVGPEETPRLQYLRFASVSLYPSNEDVSLAVS"
     CDS             1600..1626
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             1636..1662
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             2437..2457
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2458..3075
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(2528..2547)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             3082..3405
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacgaacaaa aactcatctc agaagaggat ctgaatgctg tgggccagga cacgcaggag
      121 gtcatcgtgg tgccacactc cttgcccttt aaggtggtgg tgatctcagc catcctggcc
      181 ctggtggtgc tcaccatcat ctcccttatc atcctcatca tgctttggca gaagaagcca
      241 cgtggtggct ctggaggtct cgagggaggt accatgcccg ccgaactgct gctgctgctg
      301 attgtcgctt ttgctaaccc ttcttgccag gtgctgtctt ctctgaggat ggccgctatt
      361 ctggacgatc agaccgtgtg cggaagagga gagaggctgg cactggcact ggctagggag
      421 cagatcaatg gcatcattga agtgcctgca aaggcccggg tcgaggtgga cattttcgaa
      481 ctgcagagag atagccagta cgagaccaca gacaccatgt gccagatcct gccaaaagga
      541 gtggtctccg tcctgggacc aagctcctct cctgcttctg caagtaccgt gtctcacatt
      601 tgtggcgaga aggaaatccc ccatatcaag gtcgggccag aggaaacacc caggctgcag
      661 tacctgcgct tcgcctcagt gagcctgtat ccatcaaacg aggatgtgtc actggcagtc
      721 agcggaggta gcctcgagaa gtccatgtcc agcatggtga gcgatactag ctgtaccttc
      781 ccatcatctg acggaatctt ctggaagcac tggattcaga ctaaggacgg ccagtgtggc
      841 agcccactgg tgagcacacg agacggattc atcgtgggga ttcacagcgc ctccaacttt
      901 acaaacacca ataactattt cacctcagtg ccaaagaact ttatggagct gctgaccaac
      961 caggaggccc agcagtgggt gagcgggtgg cgcctgaacg ccgattccgt gctgtggggc
     1021 gggcacaagg tgtttatgac cgcatgcggt ggcggtggct ctggaggtgg tgggtccgga
     1081 ggaggaggtg gcggttccgg cggaggtggg tctggtggag gtggatctgg aggtggtgga
     1141 tcaggaggag gaggttcagg cggccgcgag tttctggcaa ccacactgga acggatcgag
     1201 aaaaatttcg tgattactga tccgagactg cctgacaacc caatcatttt tgcgagcgat
     1261 tccttcctgc aactgacaga atattctcgg gaagagatcc tggggcgcaa ttgccgtttt
     1321 ctgcaaggac ccgagacaga ccgtgccact gttcggaaaa tcagagatgc tattgacaac
     1381 cagactgaag tgaccgttca gctgatcaat tataccaaga gcggcaagaa gttctggaac
     1441 gtgttccacc tgcaaccgat gcgcgattat aagggcgacg tccagtactt cattggcgtg
     1501 cagctggatg gcaccgaacg tcttcatggc gccgctgagc gtgaggcggt ctgcctgatc
     1561 aaaaagacag cctttcagat tgctggatcc atgcttcaac ttcctcctct tgaacgtctt
     1621 actctcatgt tgcagctacc accattagag agactaacac tgatgggcga gagcctgttc
     1681 aagggaccta gggactacaa ccctatcagt agcacaattt gtcacctgac caacgagagt
     1741 gatggccaca caacaagcct gtacggcatc gggttcggac cctttatcat caccaacaag
     1801 cacctgttca ggcggaataa tggcactctg ctggtgcaga gcctgcacgg ggtgttcaaa
     1861 gtgaagaaca caaccactct gcagcagcac ctgatcgatg ggcgggatat gatcatcatt
     1921 aggatgccca aggacttccc cccttttcct cagaaactga agttccgaga gccccagaga
     1981 gaggagagaa tctgtctggt gaccacaaac tttcagaccg agtttctggc aaccacactg
     2041 gaacggatcg agaaaaattt cgtgattact gatccgagac tgcctgacaa cccaatcatt
     2101 tttgcgagcg attccttcct gcagctgaca gaatattctc gggaagagat cctggggcgc
     2161 aattgccgtt ttctgcaggg acccgagaca gaccgtgcca ctgttcggaa aatcagagat
     2221 gctattgaca accagactga agtgaccgtt cagctgatca attataccaa gagcggcaag
     2281 aagttctgga acgtgttcca cctgcagccg atgcgcgatt ataagggcga cgtccagtac
     2341 ttcattggcg tgcagctgga tggcaccgaa cgtcttcatg gcgccgctga gcgtgaggcg
     2401 gtctgcctga tcaaaaagac agcctttcag attgctgaga acctgtactt ccagggctct
     2461 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc
     2521 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat
     2581 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg
     2641 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac
     2701 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt
     2761 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa
     2821 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta
     2881 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact
     2941 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca
     3001 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa
     3061 tgtgaaagtg ggtccaccgg tggagtgcag gtggaaacca tctccccagg agacgggcgc
     3121 accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct tgaagatgga
     3181 aagaaatttg attcctcccg ggacagaaac aagcccttta agtttatgct aggcaagcag
     3241 gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca gagagccaaa
     3301 ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat catcccacca
     3361 catgccactc tcgtcttcga tgtggagctt ctaaaactgg aatag
//
