LOCUS       sequence-410738-       13967 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Module for the expression of geneticin resistance, Nluc under PgpdA 
            promoter and luciferase under a synthetic promoter containing the 
            target sequence for gRNA1 (1xLuc)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13967)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 13967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB398
FEATURES             Location/Qualifiers
     source          1..13967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(286..1080)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(397..416)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1007..1026
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             5359..7011
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5425..5443)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    7285..7309
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8610..9239
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9668..10741
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10807..11001
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11237..11259)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11345..11485
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11400..11419
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11500..11517)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11671..12259)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11751..11770)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12347..13138)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    13924..13948
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gagagcgtca tcatgcaaca tgcatgtact gtctgatgta ttaagagtat aggggtcaac
       61 aactagagtg tctgacaggt atatctagca gtttgatttg accattcttt tattaaaaca
      121 ctgtaacccg caacgaagaa ctcctccgct tcaagtcaag aatgcgcata tgtgcgtaaa
      181 atcgacaagc ccggcggcat tccaccgagc agggtattgc ctttggcact ttacgtgcgc
      241 agaggagcct gaatgttgag tggaatgatg gtggaatgat gaagctcaga agaactcgtc
      301 aagaaggcga tagaaggcta tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
      361 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
      421 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
      481 attttccacc atgatattcg gcaagcaggc gtcgccgtgg gtcacgacga gatcctcgcc
      541 gtcgggcatc cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
      601 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
      661 gcggtgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
      721 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
      781 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
      841 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg
      901 gagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
      961 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     1021 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     1081 tttggatgct tgggtagaat aggtaagtca gattgaatct gaaataaagg gaggaagggc
     1141 gaacttaaga aggtatgacc gggtcgtcca cttaccttgc ttgacaaacg caccaagtta
     1201 tcgtgcacca agcagcagat gataataatg tcctcgttcc tgtctgctaa taagagtcac
     1261 acttcgagcg ccgccgctac tgctacaagt ggggctgatc tgaccagttg cctaaatgaa
     1321 ccatcttgtc aaacgacaca aattttgtgc tcaccgcctg gacgactaaa ccaaaatagg
     1381 cattcattgt tgacctccac tagctccagc caagcccaaa aaatgctcct tcaatatcag
     1441 ttctcctgac ggagtggcgc atgcggacag acggacggac gcagagagaa gggctgagta
     1501 ataagcgcca ctgcgccaga cagctctggc ggctctgagg tgcagtggat gattattaat
     1561 ccgggaccgg ccgcccctcc gccccgaagt ggaaaggctg gtgtgcccct cgttgaccaa
     1621 gaatctattg catcatcgga gaatatggag cttcatcgaa tcaccggcag taagcgaagg
     1681 agaatgtgaa gccaggggtg tatagccgtc ggcgaaatag catgccatta acctaggtac
     1741 agaagtccaa ttgcttccga tctggtaaaa gattcacgag atagtacctt ctccgaagta
     1801 ggtagagcga gtacccggcg cgtaagctcc ctaattggcc catccggcat ctgtagggcg
     1861 tccaaatatc gtgcctctcc tgctttgccc ggtgtatgaa accggaaagg ccgctcagga
     1921 gctggccagc ggcgcagacc gggaacacaa gctggcagtc gacccatccg gtgctctgca
     1981 ctcgacctgc tgaggtccct cagtccctgg taggcagctt tgccccgtct gtccgcccgg
     2041 tgtgtcggcg gggttgacaa ggtcgttgcg tcagtccaac atttgttgcc atattttcct
     2101 gctctcccca ccagctgctc ttttcttttc tctttctttt cccatcttca gtatattcat
     2161 cttcccatcc aagaaccttt aaaatggttt tcacccttga agatttcgtc ggcgactggc
     2221 gccaaaccgc cggatataac cttgaccagg tgctggagca gggaggagtc tcttccttgt
     2281 tccagaatct cggcgtttcc gttactccta tccagcgtat tgtgttgagc ggagagaacg
     2341 gtcttaagat tgacatccac gtcattatcc catacgaggg cctctctggc gatcaaatgg
     2401 gacagatcga gaaaattttc aaggtcgttt atccagtcga cgatcatcac tttaaagtta
     2461 tcctgcacta cggtactctt gtgatcgatg gcgtcacccc caacatgatt gactacttcg
     2521 gccgacccta cgagggtatc gctgtgttcg acggtaagaa gattactgtg accggtactt
     2581 tgtggaatgg aaacaagatt atcgatgagc gcctcatcaa ccctgatggc tccttgctgt
     2641 ttcgtgttac cattaacgga gttactggtt ggcgcctttg cgaacgtatt ctcgcataag
     2701 cttcatcatt ccaccatcat tccactcaac attcaggctc ctctgcgcac gtaaagtgcc
     2761 aaaggcaata ccctgctcgg tggaatgccg ccgggcttgt cgattttacg cacatatgcg
     2821 cattcttgac ttgaagcgga ggagttcttc gttgcgggtt acagtgtttt aataaaagaa
     2881 tggtcaaatc aaactgctag atatacctgt cagacactct agttgttgac ccctatactc
     2941 ttaatacatc agacagtaca tgcatgttgc atgatgacgc tctccgtcag gagtggctat
     3001 tgttgttatc atcaaaatct atattgatac tattatactc gatatgttat agactttatt
     3061 aaaaaaaact ttgtttgttt gcagaaagtg gatttttaat caaatttaat tgtgatgatt
     3121 acaatgtcaa ttggttatgc aagatattaa ttaatatgtt taagattttt cacttttaat
     3181 aagtaaaata aaatacgtca aaaaaatttc ctaaagataa tgccaaaaag gttaaattat
     3241 agaaattaaa ctaaaatata ttaaaagttg acttaaaaat gcaatgttga ccaccgacct
     3301 aggggaaaac aaatttaccg tatctgggat agaatctctt ctgtatcatc tcagataaag
     3361 attactacat aaacacactt tacagtttta cgacgcccaa aattcaattc ccggagatta
     3421 aaaaccctaa aacaaacact ggcgattcga ttttccctcc caattcaaat catctcttaa
     3481 tcttcttctt ctcactatcc tctcaaattc gccgccgatc ttccatcaat tttgatttct
     3541 cgcaccgaaa tttcgacttt tcatctccta tcgacgaaac cctaattttt cacactccag
     3601 aaatcgaact ttgacgacga tgttaatggc taaaccccgc tgtcaggagt ctcaatggta
     3661 actttactct ttatttaacc atacattttt ttttattttt ttcactttgt tcttcatcca
     3721 ctattgttct ttgttcatct tgaacaaaag ctccctcctt ctttgttctt catccaccat
     3781 tgttcttcat caatcatttc gctgtcagga gtgtttcatg tccaagcgaa aagccgctct
     3841 acggaatgga tctacgttac tgcctgcata aggagatcgg tgtagccaag gacgaaagcg
     3901 accctaggtt ctaaccgtcg actttggcgg aaaggtttca ctcaggaagc agacactgat
     3961 tgacacggtt tagcagaacg tttgaggact aggtcaaatt gagtggttta atatcggcat
     4021 gtctggcttt aaaattcagt atagtgcgct gatcggagat gaattaaaaa cacgagttcc
     4081 caaaaccagg cgggctcgcc acgtcggcta atcctggtac attatgtgaa caatgttctg
     4141 aagaaaattt gtgaaagaag gacgggtcat cgcctactat tagcaacaac ggtcggccac
     4201 accttccatt gtcgtggcca cgctcggatt acacggcaga ggtgcttgtg ttccgacagg
     4261 ctagcatatt atcctaaggc gttaccccaa tcgtttaccg tcggatttgc tatagcccct
     4321 gaacgctaca tgtacgaaac catgttatgt atgcactagg tcaacaatag gacatagcct
     4381 tgtagttaac acgtagcccg gtcgtataag tacagtagac ccttcgccgg catcctattt
     4441 attaagttat ttctacagca aaacgatcat atgcagatcc gcagtgggcg gtagacacac
     4501 gtccacccgg ctgctctgta acagggacta aagaggtgat gattatcgtg tgtgccccgt
     4561 tatggtcgtg ttcgatcaga gcgcccttgc gagcagtcgt atgctttctc gaattccgag
     4621 cggttaagcg tgacagtccc agcgaaccca caaaacgtga tcgcagtcca tgcgatcata
     4681 cgcaagaagg aaggtcccca tacaccgacg caccagttta cacgccgtat gcataaacga
     4741 gctgcacaaa cgagagtgtt tgaactggac ctctagttcc tctacaaaga acaccttgac
     4801 ctgttgcgaa gttgccttgc ctagatgcaa tgtcggacgt atcacttttg cctcaacgac
     4861 tgctgctttc gctgtaaccc aagacagaca acagtaaccg ccttttgaag gcaagtcctc
     4921 cgcctgtgac taactgtgcc aaatcgtctt ccaaacccct tatccaattt aactcaccaa
     4981 attattgcga tacagaccct aatttcacat catatgacac taattgcctc tgccaaaatt
     5041 ctgtcctcaa tgacgctgta tctaatagaa tcttgggaca tgggcgtttg gcactaccga
     5101 cacgaacctc agttccatct tctctcacca accagtctga aacactggaa atgggacgtg
     5161 aggtaggatc ggttgtccta tctttcgttg ccgactgcgc agaattccct gtcacctttc
     5221 acacagagct catgatctgg tttataaagg cggcttcatg accctcaatt ccatatagta
     5281 tcactcccat cacagcattt cgatatcttc aaccacttta accttctcca gaggatcatc
     5341 atctcaagcc cttcataaat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc
     5401 tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc
     5461 ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct
     5521 gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca
     5581 aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc
     5641 gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg
     5701 ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa
     5761 aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct
     5821 aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc
     5881 ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg
     5941 atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact
     6001 gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat
     6061 actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga
     6121 tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg
     6181 aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc
     6241 ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct
     6301 ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca
     6361 ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag
     6421 ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg
     6481 gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt
     6541 cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag
     6601 gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc
     6661 gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg
     6721 gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac
     6781 gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg
     6841 gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga
     6901 ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa
     6961 atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta atgagcttac
     7021 ttaacgttac tgaaatcatc aaacagcttg acgaatctgg atataagatc gttggtgtcg
     7081 atgtcagctc cggagttgag acaaatggtg ttcaggatct cgataagata cgttcatttg
     7141 tccaagcagc aaagagtgcc ttctagtgat ttaatagctc catgtcaaca agaataaaac
     7201 gcgtttcggg tttacctctt ccagatacag ctcatctgca atgcatcgac cgtctgagac
     7261 atgcgctgtc atgagaccgg atcctgacag gatatattgg cgggtaaacc taagagaaaa
     7321 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     7381 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc
     7441 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtcat ctgaaaacga
     7501 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt
     7561 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta
     7621 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga
     7681 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc
     7741 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct
     7801 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga
     7861 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga
     7921 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat
     7981 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa
     8041 ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc
     8101 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt
     8161 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc
     8221 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc
     8281 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg
     8341 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg
     8401 cccgcgcacc tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag
     8461 ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg
     8521 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga
     8581 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag
     8641 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg
     8701 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc
     8761 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga
     8821 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg
     8881 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg
     8941 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg
     9001 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg
     9061 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc
     9121 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg
     9181 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag
     9241 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag
     9301 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg
     9361 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca
     9421 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag
     9481 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag
     9541 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag
     9601 gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac tggaaccccc aagcccgagg
     9661 aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga
     9721 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga
     9781 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca
     9841 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga
     9901 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt
     9961 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct
    10021 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta
    10081 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg
    10141 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg
    10201 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa
    10261 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt
    10321 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc
    10381 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa
    10441 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt
    10501 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac
    10561 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa
    10621 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg
    10681 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta
    10741 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggact
    10801 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc
    10861 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat
    10921 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa
    10981 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc
    11041 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc
    11101 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc
    11161 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg
    11221 gtgatgacgg tgaaaacctc tgacacatgc agctcccggt gacggtcaca gcttgtctgt
    11281 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
    11341 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
    11401 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    11461 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
    11521 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    11581 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    11641 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    11701 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    11761 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    11821 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    11881 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    11941 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    12001 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    12061 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
    12121 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    12181 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    12241 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    12301 gaacgaaaac tcacgttaag ggattttggt catgcattct aggtgattat ttgccgacta
    12361 ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg
    12421 ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat
    12481 actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc
    12541 cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcaccag
    12601 cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct
    12661 gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt
    12721 ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac
    12781 ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac
    12841 gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc
    12901 tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt
    12961 catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc
    13021 catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga
    13081 tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa
    13141 tgtttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca
    13201 tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta
    13261 ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc
    13321 gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac
    13381 gaaccgaaca ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc
    13441 acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg gaacagaact
    13501 tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca
    13561 agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt
    13621 tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac
    13681 cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg
    13741 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa
    13801 catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg
    13861 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
    13921 tggtggcagg atatattgtg gtgtaaacat aacggatccg gtctcag
//
