LOCUS       sequence-411208-       10136 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human EYA1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10136)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 10136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-EYA1
FEATURES             Location/Qualifiers
     source          1..10136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2403..2421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2445..2463
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2487..2505
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2529..2547
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2571..2589
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2613..2631
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2664..2702
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2699..2723
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4617..5205
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4670..4690)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5088..5099)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5208..5224)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5209..5225)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5730..5910
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5936..5953)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5942..6166
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6212..6640
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6299..6318)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6509..6530
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6649..6669)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6654..6983
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6834..6969
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6896..6915
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7031..7078
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7097..7471
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7601..7722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7638..7657)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7692..7711
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7771..7787)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7771..7787)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7784..7806)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7795..7811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7819..7825,7826..7843,7844..7849))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7864..7885
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8002..8019)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8173..8761)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8253..8272)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8932..9723,9724..9792))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9555..9574
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9793..9897)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9972..9991)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
     2701 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     2761 gaagacaccg ggaccgatcc agcctccgcg gccccgaatt caccggttct agaatggaaa
     2821 tgcaggatct aaccagcccg catagccgtc tgagtggtag tagtgaatcc cccagtggcc
     2881 ccaaactcgg taactctcat ataaatagta attccatgac tcccaatggc accgaagtta
     2941 aaacagagcc aatgagcagc agtgaaacag cttcaacgac agccgacggg tctttaaaca
     3001 atttctcagg ttcagcaatt gggagcagta gtttcagccc acgaccaact caccagttct
     3061 ctccaccaca gatttaccct tccaacagac catacccaca tattctccct accccttcct
     3121 cacaaactat ggctgcatat gggcaaacac agtttaccac aggaatgcaa caagctacag
     3181 cctatgccac gtacccacag ccaggacagc cgtacggcat ttcctcatat ggtgcattgt
     3241 gggcaggcat caagactgaa ggtggattgt cacagtctca gtcacctgga cagacaggat
     3301 ttctcagcta tggcacaagc ttcagtaccc ctcaacctgg acaggcacca tacagctacc
     3361 agatgcaagg tagcagtttt acaacatcat caggaatata tacaggaaat aattcactca
     3421 caaattcctc tggatttaat agttcacagc aggactatcc gtcttatccc agttttggcc
     3481 agggtcagta cgcacagtat tataacagct caccgtatcc agcacattat atgaccagca
     3541 gcaacaccag cccaacgaca ccatccacca atgccactta ccagcttcaa gaaccgccat
     3601 ctggcatcac cagccaagca gttacggatc ccacagcaga gtacagcaca atccacagcc
     3661 catcaacacc cattaaagat tcagattctg atcgattgcg tcgaggttca gatgggaaat
     3721 cacgtggacg gggccgaaga aacaataatc cttcacctcc cccagattct gatcttgaga
     3781 gagtgttcat ctgggacttg gatgagacaa tcattgtttt ccactccttg cttactgggt
     3841 cctacgccaa cagatatggg agggatccac ccacttcagt ttcccttgga ctgcgaatgg
     3901 aagaaatgat tttcaacttg gcagacacac atttattttt taatgactta gaagaatgtg
     3961 accaagtcca tatagatgat gtttcttcag atgataacgg acaggaccta agcacatata
     4021 actttggaac agatggcttt cctgctgcag caaccagtgc taacttatgt ttggcaactg
     4081 gtgtacgggg cggtgtggac tggatgagaa agttggcctt ccgctacaga cgggtaaaag
     4141 agatctacaa cacctacaaa aataatgttg gaggtctgct tggtccagct aagagggaag
     4201 cctggctgca gttgagggcc gaaattgaag ccctgaccga ctcctggttg acactggccc
     4261 tgaaagcact ctcgctcatt cactcccgga caaactgtgt gaatatttta gtaacaacta
     4321 ctcagctcat cccagcattg gcgaaagtcc tgctgtatgg gttaggaatt gtatttccaa
     4381 tagaaaatat ttacagtgca actaaaatag gaaaagaaag ctgttttgag agaataattc
     4441 aaaggtttgg aagaaaagtg gtgtatgttg ttataggaga tggtgtagaa gaagaacaag
     4501 gagcaaaaaa gcacgcgatg cccttctgga ggatctccag ccactcggac ctcatggccc
     4561 tgcaccatgc cttggaactg gagtacctgt aattcgaaga tatcaagctt atcgataatc
     4621 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     4681 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     4741 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4801 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4861 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4921 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4981 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     5041 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5101 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5161 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     5221 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     5281 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     5341 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     5401 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     5461 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
     5521 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
     5581 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
     5641 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
     5701 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
     5761 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     5821 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     5881 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
     5941 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     6001 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     6061 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     6121 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     6181 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     6241 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6301 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6361 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6421 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6481 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6541 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6601 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     6661 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6721 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6781 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     6841 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6901 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     6961 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     7021 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     7081 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     7141 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     7201 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     7261 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     7321 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     7381 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     7441 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     7501 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     7561 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     7621 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7681 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     7741 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7801 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7861 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7921 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7981 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8041 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8101 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8161 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8221 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8281 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8341 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8401 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8461 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8521 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8581 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8641 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8701 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8761 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8821 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8881 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8941 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9001 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9061 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9121 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9181 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9241 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9301 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9361 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9421 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9481 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9541 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9601 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9661 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9721 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9781 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9841 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9901 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     9961 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
    10021 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
    10081 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcg
//
