LOCUS       sequence-411210-       11768 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human HDAC5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11768)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 11768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-HDAC5
FEATURES             Location/Qualifiers
     source          1..11768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    6249..6837
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6302..6322)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6720..6731)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6840..6856)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6841..6857)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7362..7542
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7568..7585)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7574..7798
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7844..8272
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7931..7950)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8141..8162
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8281..8301)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8286..8615
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8466..8601
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8528..8547
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8663..8710
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8729..9103
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9233..9354
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9270..9289)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9324..9343
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9403..9419)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9403..9419)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9416..9438)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9427..9443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9451..9457,9458..9475,9476..9481))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9496..9517
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9634..9651)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9805..10393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9885..9904)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10564..11355,11356..11424))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11187..11206
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11425..11529)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11604..11623)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcaccggtt ctagaatgaa ctctcccaac gagtcggatg
     2881 ggatgtcagg tcgggaacca tccttggaaa tcctgccgcg gacttctctg cacagcatcc
     2941 ctgtgacagt ggaggtgaag ccggtgctgc caagagccat gcccagttcc atggggggtg
     3001 ggggtggagg cagccccagc cctgtggagc tacggggggc tctggtgggc tctgtggacc
     3061 ccacactgcg ggagcagcaa ctgcagcagg agctcctggc gctcaagcag cagcagcagc
     3121 tgcagaagca gctcctgttc gctgagttcc agaaacagca tgaccacctg acaaggcagc
     3181 atgaggtcca gctgcagaag cacctcaagc agcagcagga gatgctggca gccaagcagc
     3241 agcaggagat gctggcagcc aagcggcagc aggagctgga gcagcagcgg cagcgggagc
     3301 agcagcggca ggaagagctg gagaagcagc ggctggagca gcagctgctc atcctgcgga
     3361 acaaggagaa gagcaaagag agtgccattg ccagcactga ggtaaagctg aggctccagg
     3421 aattcctctt gtcgaagtca aaggagccca caccaggcgg cctcaaccat tccctcccac
     3481 agcaccccaa atgctgggga gcccaccatg cttctttgga ccagagttcc cctccccaga
     3541 gcggcccccc tgggacgcct ccctcctaca aactgccttt gcctgggccc tacgacagtc
     3601 gagacgactt ccccctccgc aaaacagcct ctgaacccaa cttgaaagtg cgttcaaggc
     3661 taaaacagaa ggtggctgag cggagaagca gtcccctcct gcgtcgcaag gatgggactg
     3721 ttattagcac ctttaagaag agagctgttg agatcacagg tgccgggcct ggggcgtcgt
     3781 ccgtgtgtaa cagcgcaccc ggctccggcc ccagctctcc caacagctcc cacagcacca
     3841 tcgctgagaa tggctttact ggctcagtcc ccaacatccc cactgagatg ctccctcagc
     3901 accgagccct ccctctggac agctccccca accagttcag cctctacacg tctccttctc
     3961 tgcccaacat ctccctaggg ctgcaggcca cggtcactgt caccaactca cacctcactg
     4021 cctccccgaa gctgtcgaca cagcaggagg ccgagaggca ggccctccag tccctgcggc
     4081 agggtggcac gctgaccggc aagttcatga gcacatcctc tattcctggc tgcctgctgg
     4141 gcgtggcact ggagggcgac gggagccccc acgggcatgc ctccctgctg cagcatgtgc
     4201 tgttgctgga gcaggcccgg cagcagagca ccctcattgc tgtgccactc cacgggcagt
     4261 ccccactagt gacgggtgaa cgtgtggcca ccagcatgcg gacggtaggc aagctcccgc
     4321 ggcatcggcc cctgagccgc actcagtcct caccgctgcc gcagagtccc caggccctgc
     4381 agcagctggt catgcaacaa cagcaccagc agttcctgga gaagcagaag cagcagcagc
     4441 tacagctggg caagatcctc accaagacag gggagctgcc caggcagccc accacccacc
     4501 ctgaggagac agaggaggag ctgacggagc agcaggaggt cttgctgggg gagggagccc
     4561 tgaccatgcc ccgggagggc tccacagaga gtgagagcac acaggaagac ctggaggagg
     4621 aggacgagga agaggatggg gaggaggagg aggattgcat ccaggttaag gacgaggagg
     4681 gcgagagtgg tgctgaggag gggcccgact tggaggagcc tggtgctgga tacaaaaaac
     4741 tgttctcaga tgcccagccg ctgcagcctt tgcaggtgta ccaggcgccc ctcagcctgg
     4801 ccactgtgcc ccaccaggcc ctgggccgta cccagtcctc ccctgctgcc cctgggggca
     4861 tgaagagccc cccagaccag cccgtcaagc acctcttcac cacaggtgtg gtctacgaca
     4921 cgttcatgct aaagcaccag tgcatgtgcg ggaacacaca cgtgcaccct gagcatgctg
     4981 gccggatcca gagcatctgg tcccggctgc aggagacagg cctgcttagc aagtgcgagc
     5041 ggatccgagg tcgcaaagcc acgctagatg agatccagac agtgcactct gaataccaca
     5101 ccctgctcta tgggaccagt cccctcaacc ggcagaagct agacagcaag aagttgctcg
     5161 gccccatcag ccagaagatg tatgctgtgc tgccttgtgg gggcatcggg gtggacagtg
     5221 acaccgtgtg gaatgagatg cactcctcca gtgctgtgcg catggcagtg ggctgcctgc
     5281 tggagctggc cttcaaggtg gctgcaggag agctcaagaa tggatttgcc atcatccggc
     5341 ccccaggaca ccacgccgag gaatccacag ccatgggatt ctgcttcttc aactctgtag
     5401 ccatcaccgc aaaactccta cagcagaagt tgaacgtggg caaggtcctc atcgtggact
     5461 gggacattca ccatggcaat ggcacccagc aggcgttcta taatgacccc tctgtgctct
     5521 acatctctct gcatcgctat gacaacggga acttctttcc aggctctggg gctcctgaag
     5581 aggttggtgg aggaccaggc gtggggtaca atgtgaacgt ggcatggaca ggaggtgtgg
     5641 acccccccat tggagacgtg gagtacctta cagccttcag gacagtggtg atgcccattg
     5701 cccacgagtt ctcacctgat gtggtcctag tctccgccgg gtttgatgct gttgaaggac
     5761 atctgtctcc tctgggtggc tactctgtca ccgccagatg ttttggccac ttgaccaggc
     5821 agctgatgac cctggcaggg ggccgggtgg tgctggccct ggagggaggc catgacttga
     5881 ccgccatctg tgatgcctct gaggcttgtg tctcggctct gctcagtgta gagctgcagc
     5941 ccttggatga ggcagtcttg cagcaaaagc ccaacatcaa cgcagtggcc acgctagaga
     6001 aagtcatcga gatccagagc aaacactgga gctgtgtgca gaagttcgcc gctggtctgg
     6061 gccggtccct gcgagaggcc caagcaggtg agaccgagga ggccgagact gtgagcgcca
     6121 tggccttgct gtcggtgggg gccgagcagg cccaggctgc ggcagcccgg gaacacagcc
     6181 ccaggccggc agaggagccc atggagcagg agcctgccct gtgattcgaa gatatcaagc
     6241 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6301 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6361 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6421 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6481 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6541 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6601 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     6661 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6721 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6781 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     6841 gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     6901 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     6961 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7021 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     7081 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
     7141 ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     7201 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
     7261 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
     7321 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     7381 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7441 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7501 gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc
     7561 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     7621 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     7681 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     7741 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     7801 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     7861 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7921 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7981 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     8041 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     8101 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     8161 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     8221 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     8281 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     8341 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     8401 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     8461 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8521 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     8581 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     8641 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     8701 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
     8761 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
     8821 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
     8881 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
     8941 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
     9001 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
     9061 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
     9121 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     9181 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     9241 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     9301 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     9361 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     9421 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     9481 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     9541 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     9601 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     9661 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     9721 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     9781 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9841 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9901 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9961 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    10021 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    10081 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    10141 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    10201 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    10261 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    10321 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    10381 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    10441 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    10501 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    10561 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    10621 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    10681 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    10741 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    10801 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    10861 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    10921 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    10981 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    11041 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    11101 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    11161 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    11221 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    11281 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    11341 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    11401 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    11461 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    11521 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
    11581 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
    11641 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
    11701 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
    11761 ctgcttcg
//
