LOCUS       sequence-412212-       12113 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human NOTCH2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 12113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-NOTCH2
FEATURES             Location/Qualifiers
     source          1..12113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4585..4594
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6594..7182
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6647..6667)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7065..7076)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7185..7201)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7186..7202)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7707..7887
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7913..7930)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7919..8143
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8189..8617
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8276..8295)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8486..8507
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8626..8646)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8631..8960
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8811..8946
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8873..8892
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9008..9055
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9074..9448
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9578..9699
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9615..9634)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9669..9688
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9748..9764)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9748..9764)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9761..9783)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9772..9788
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9796..9802,9803..9820,9821..9826))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9841..9862
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9979..9996)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10150..10738)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10230..10249)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10909..11700,11701..11769))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11532..11551
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11770..11874)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11949..11968)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcaccggta tgcccgccct gcgccccgct ctgctgtggg
     2881 cgctgctggc gctctggctg tgctgcgcgg cccccgcgca tgcattgcag tgtcgagatg
     2941 gctatgaacc ctgtgtaaat gaaggaatgt gtgttaccta ccacaatggc acaggatact
     3001 gcaaatgtcc agaaggcttc ttgggggaat attgtcaaca tcgagacccc tgtgagaaga
     3061 accgctgcca gaatggtggg acttgtgtgg cccaggccat gctggggaaa gccacgtgcc
     3121 gatgtgcctc agggtttaca ggagaggact gccagtactc aacatctcat ccatgctttg
     3181 tgtctcgacc ctgcctgaat ggcggcacat gccatatgct cagccgggat acctatgagt
     3241 gcacctgtca agtcgggttt acaggtaagg agtgccaatg gacggatgcc tgcctgtctc
     3301 atccctgtgc aaatggaagt acctgtacca ctgtggccaa ccagttctcc tgcaaatgcc
     3361 tcacaggctt cacagggcag aaatgtgaga ctgatgtcaa tgagtgtgac attccaggac
     3421 actgccagca tggtggcacc tgcctcaacc tgcctggttc ctaccagtgc cagtgccctc
     3481 agggcttcac aggccagtac tgtgacagcc tgtatgtgcc ctgtgcaccc tcaccttgtg
     3541 tcaatggagg cacctgtcgg cagactggtg acttcacttt tgagtgcaac tgccttccag
     3601 gttttgaagg gagcacctgt gagaggaata ttgatgactg ccctaaccac aggtgtcaga
     3661 atggaggggt ttgtgtggat ggggtcaaca cttacaactg ccgctgtccc ccacaatgga
     3721 caggacagtt ctgcacagag gatgtggatg aatgcctgct gcagcccaat gcctgtcaaa
     3781 atgggggcac ctgtgccaac cgcaatggag gctatggctg tgtatgtgtc aacggctgga
     3841 gtggagatga ctgcagtgag aacattgatg attgtgcctt cgcctcctgt actccaggct
     3901 ccacctgcat cgaccgtgtg gcctccttct cttgcatgtg cccagagggg aaggcaggtc
     3961 tcctgtgtca tctggatgat gcatgcatca gcaatccttg ccacaagggg gcactgtgtg
     4021 acaccaaccc cctaaatggg caatatattt gcacctgccc acaaggctac aaaggggctg
     4081 actgcacaga agatgtggat gaatgtgcca tggccaatag caatccttgt gagcatgcag
     4141 gaaaatgtgt gaacacggat ggcgccttcc actgtgagtg tctgaagggt tatgcaggac
     4201 ctcgttgtga gatggacatc aatgagtgcc attcagaccc ctgccagaat gatgctacct
     4261 gtctggataa gattggaggc ttcacatgtc tgtgcatgcc aggtttcaaa ggtgtgcatt
     4321 gtgaattaga aataaatgaa tgtcagagca acccttgtgt gaacaatggg cagtgtgtgg
     4381 ataaagtcaa tcgtttccag tgcctgtgtc ctcctggttt cactgggcca gtttgccaga
     4441 ttgatattga tgactgttcc agtactccgt gtctgaatgg ggcaaagtgt atcgatcacc
     4501 cgaatggcta tgaatgccag tgtgccacag gtttcactgg tgtgttgtgt gaggagaaca
     4561 ttgacaactg tgaccccgat ccttgccacc atggtcagtg tcaggatggt attgattcct
     4621 acacctgcat ctgcaatccc gggtacatgg gcgccatctg cagtgaccag attgatgaat
     4681 gttacagcag cccttgcctg aacgatggtc gctgcattga cctggtcaat ggctaccagt
     4741 gcaactgcca gccaggcacg tcaggggtta attgtgaaat taattttgat gactgtgcaa
     4801 gtaacccttg tatccatgga atctgtatgg atggcattaa tcgctacagt tgtgtctgct
     4861 caccaggatt cacagggcag agatgtaaca ttgacattga tgagtgtgcc tccaatccct
     4921 gtcgcaaggg tgcaacatgt atcaacggtg tgaatggttt ccgctgtata tgccccgagg
     4981 gaccccatca ccccagctgc tactcacagg tgaacgaatg cctgagcaat ccctgcatcc
     5041 atggaaactg tactggaggt ctcagtggat ataagtgtct ctgtgatgca ggctgggttg
     5101 gcatcaactg tgaagtggac aaaaatgaat gcctttcgaa tccgtgccag aatggaggaa
     5161 cttgtgacaa tctggtgaat ggatacaggt gtacttgcaa gaagggcttt aaaggctata
     5221 actgccaggt gaatattgat gaatgtgcct caaatccatg cctgaaccaa ggaacctgct
     5281 ttgatgacat aagtggctac acttgccact gtgtgctgcc atacacaggc aagaattgtc
     5341 agacagtatt ggctccctgt tccccaaacc cttgtgagaa tgctgctgtt tgcaaagagt
     5401 caccaaattt tgagagttat acttgcttgt gtgctcctgg ctggcaaggt cagcggtgta
     5461 ccattgacat tgacgagtgt atctccaagc cctgcatgaa ccatggtctc tgccataaca
     5521 cccagggcag ctacatgtgt gaatgtccac caggcttcag tggtatggac tgtgaggagg
     5581 acattgatga ctgccttgcc aatccttgcc agaatggagg ttcctgtatg gatggagtga
     5641 atactttctc ctgcctctgc cttccgggtt tcactgggga taagtaccag acagacatga
     5701 atgagtgtct gagtgaaccc tgtaagaatg gagggacctg ctctgactac gtcaacagtt
     5761 acacttgcaa gtgccaggca ggatttgatg gagtccattg tgagaacaac atcaatgagt
     5821 gcactgagag ctcctgtttc aatggtggca catgtgttga tgggattaac tccttctctt
     5881 gcttgtgccc tgtgggtttc actggatcct tctgcctcca tgagatcaat gaatgcagct
     5941 ctcatccatg cctgaatgag ggaacgtgtg ttgatggcct gggtacctac cgctgcagct
     6001 gccccctggg ctacactggg aaaaactgtc agaccctggt gaatctctgc agtcggtctc
     6061 catgtaaaaa caaaggtact tgcgttcaga aaaaagcaga gtcccagtgc ctatgtccat
     6121 ctggatgggc tggtgcctat tgtgacgtgc ccaatgtctc ttgtgacata gcagcctcca
     6181 ggagaggtgt gcttgttgaa cacttgtgcc agcactcagg tgtctgcatc aatgctggca
     6241 acacgcatta ctgtcagtgc cccctgggct atactgggag ctactgtgag gagcaactcg
     6301 atgagtgtgc gtccaacccc tgccagcacg gggcaacatg cagtgacttc attggtggat
     6361 acagatgcga gtgtgtccca ggctatcagg gtgtcaactg tgagtatgaa gtggatgagt
     6421 gccagaatca gccctgccag aatggaggca cctgtattga ccttgtgaac catttcaagt
     6481 gctcttgccc accaggcact cggggtatga aatcatcctt atccattttc catccagggc
     6541 attgtcttaa gttataatct agagctagct tcgaagatat caagcttatc gataatcaac
     6601 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6661 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     6721 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6781 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     6841 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6901 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6961 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     7021 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7081 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     7141 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     7201 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     7261 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     7321 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7381 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     7441 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     7501 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     7561 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     7621 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     7681 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     7741 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7801 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7861 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
     7921 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     7981 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     8041 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     8101 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     8161 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     8221 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     8281 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8341 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8401 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8461 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     8521 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     8581 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     8641 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     8701 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     8761 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     8821 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     8881 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8941 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     9001 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     9061 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     9121 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     9181 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     9241 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     9301 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     9361 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     9421 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
     9481 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     9541 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     9601 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     9661 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     9721 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     9781 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9841 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9901 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9961 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    10021 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    10081 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    10141 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    10201 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    10261 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    10321 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10381 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10441 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10501 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10561 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    10621 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10681 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10741 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10801 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10861 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10921 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10981 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    11041 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    11101 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    11161 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    11221 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    11281 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    11341 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    11401 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    11461 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11521 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11581 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11641 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11701 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11761 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11821 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11881 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
    11941 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
    12001 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
    12061 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcg
//
