LOCUS       sequence-412243-        8699 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses the green 5-HT sensor GRAB_g5-HT2m in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8699)
  AUTHORS   Deng F, Wan J, Li G, Dong H, Xia X, Wang Y, Li X, Zhuang C, Zheng Y,
            Liu L, Yan Y, Feng J, Zhao Y, Xie H, Li Y
  TITLE     Dual-color GRAB sensors for monitoring spatiotemporal serotonin 
            release in vivo
  JOURNAL   bioRxiv 2023.05.27.542566
REFERENCE   2  (bases 1 to 8699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.27.542566"
COMMENT     Sequence Label: pDisplay-GRAB_g5-HT2m
FEATURES             Location/Qualifiers
     source          1..8699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        380..583
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     534..554
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     628..647
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        628..646
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     727..789
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     protein_bind    790..814
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1878..1899
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2005..2026)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2266..2285)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    join(2888..3461,3462..3464,3465..3473)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3055..3072)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3281..3300
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3474..4181
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3619..3637)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3887..3906
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4239..4268
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4272..4418
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4440..4457)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4446..4670
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4802..5390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4882..4901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5719..5766
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5791..5810
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5998..6792)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTYGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6109..6128)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6719..6738
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(6891..6910)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        6989..7180
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     7137..7157
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(7240..8031,8032..8100))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7863..7882
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8101..8205)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8232..8687
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8319..8338)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8529..8550
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        join(8699,1..379)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
       61 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      121 cgacccctgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      181 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      241 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      301 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      361 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      421 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      481 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      541 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
      601 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagcttg
      661 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttggggata
      721 tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt tccaggttcc
      781 actggtgaca caagtttgta caaaaaagtt ggcaccaccg gtgacaaact tgatgctaat
      841 gtgagttcca acgagggttt caggtccgtg gagaaggtcg tgctgctcac gttccttgca
      901 gtggttatcc tgatggccat cttgggcaac ctgctggtga tggtggctgt gtgcagggac
      961 aggcagctca ggaaaataaa aaccaactat ttcattgtgt ctctcgcctt tgctgacctg
     1021 ctggtttcgg tgctggtgat gccctttggt gccattgagc tggtccaaga catctgggct
     1081 tatggggaga tgttctgcct ggtccggacc tctctggatg tctaccttac cacagcatcg
     1141 atctttcacc tgtgctgtat ttccctggac aggtattacg ccatctgctg ccagcctttg
     1201 gtttatagga acaagatgac ccctctacgc atcgcattaa tgttgggagg ctgctgggtc
     1261 cttcccatgt ttatatcttt tctccccata atgcaaggct ggaacaacat cggcatagtt
     1321 gatgtgatag agaaaaggaa attcagccac aactctaact ccacgtggtg tgtcttcatg
     1381 gtcaacaagc cctatgctat cacctgctct gtggtggccg gctacatccc gtttctcctc
     1441 atggtgctgg cctattaccg aatctatgtc gtggctaagc ggggtccgga cgccgtcgcc
     1501 gcgccgccgg ggggcaccga gcgcaggccc aacggtctgg gccccgagcg cagcgcgggc
     1561 ccggggggcg cagaggccga accgctgccc acccagctca acggcgcccc tggcgagccc
     1621 gcgccggccg ggccgcgcga caccgacgcg ctggacctgg aggagggagg aaacgtctat
     1681 atcaaggccg acaagcagaa gaacggcatc aaggcgatct tccacatccg ccacaacatc
     1741 gaggacggcg gcgtgcagct cgccatccac taccagcaga acacccccat cggcgacggc
     1801 cccgtgctgc tgcccgacaa ccactacctg agcgtgcagt ccaaactttc gaaagacccc
     1861 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1921 ggcatggacg agctgtacaa gggcggtacc ggagggagca tggtgagaaa gggcgaggag
     1981 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     2041 ttcagcgtgc ggggcgaggg tgagggcgat gccaccaatg gcaagctgac cctgaagttc
     2101 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     2161 ggcgtgcagt gcttcagtcg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     2221 gccatgcccg aaggctacgt gcaggagcgc accatcttct tcaaggacga cggcaactac
     2281 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     2341 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacaccgga
     2401 gcagcagcac gcttccgctg gaggacagag accaaggcag ccaaggtgtt atgtgtcatc
     2461 atgggctgct tctgtttctg ctgggccccc ttctttgtca ccaatattgt ggaccctttc
     2521 atagactaca ctgtccccga gcaggtgtgg actgctttcc tctggcttgg ctatatcaat
     2581 tcggggttga acccttttct ctatgccttc ttgaataagt ctttcagacg tgccttcctc
     2641 atcatcctcc gttgcaatta taaggtagag aaaaagcctc ctgtcaggca gattccaaga
     2701 gttgccgcca ctgctttgtc tgggagggag cttaatgtta acatttatcg gcataccaat
     2761 gaaccggtga tcgagaaagc cagtgacaat gagcccggta tagagatgca agttgagaat
     2821 ttagagttac cagtaaatcc ctccagtgtg gttagcgaaa ggattagcag tgtgtgataa
     2881 gctagcgccc ctctccctcc cccccccnta acgttactgg ccgaagccgc ttggaataag
     2941 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     3001 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     3061 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     3121 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     3181 ggtgcctctg cggccaacgc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     3241 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     3301 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     3361 tcggtacaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     3421 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggtga
     3481 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     3541 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     3601 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     3661 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     3721 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     3781 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     3841 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     3901 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     3961 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     4021 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     4081 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     4141 agggccgcca ctccaccggc ggcatggacg agctgtacaa ggatggtaaa aagaagaaaa
     4201 agaagtcaaa gacaaagtgt gtaattatgt aagtcgacga acaaaaactc atctcagaag
     4261 aggatctgaa tgctgtgggc caggacacgc aggaggtcat cgtggtgcca cactccttgc
     4321 cctttaaggt ggtggtgatc tcagccatcc tggccctggt ggtgctcacc atcatctccc
     4381 ttatcatcct catcatgctt tggcagaaga agccacgtta ggcggccgct cgagatcagc
     4441 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4501 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4561 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4621 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     4681 ggaaagaacc agtggcggta atacggttat ccacagaatc gggggataac gcaggaaaga
     4741 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4801 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4861 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4921 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4981 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5041 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5101 actatcgtct tgagtccaac ccggtaagac gcgacttatc gccactggca gcagccactg
     5161 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5221 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     5281 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5341 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5401 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5461 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     5521 aatcaatcta aagtatatat gagtaacctg aggctatggc agggcctgcc gccccgacgt
     5581 tggctgcgag ccctgggcct tcacccgaac ttggggggtg gggtggggaa aaggaagaaa
     5641 cgcgggcgta ttggccccaa tggggtctcg gtggggtatc gacagagtgc cagccctggg
     5701 accgaacccc gcgtttatga acaaacgacc caacaccgtg cgttttattc tgtcttttta
     5761 ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca gttagcctcc
     5821 ccctagggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg
     5881 cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat
     5941 ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca
     6001 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     6061 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     6121 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     6181 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat aggtcacgac
     6241 gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag
     6301 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     6361 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     6421 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     6481 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     6541 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     6601 tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     6661 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     6721 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     6781 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcgatctttg caaaagccta
     6841 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccggg cctcggcctc
     6901 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     6961 taggggcgga atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     7021 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     7081 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     7141 taactgacac acattccaca gctggttctt tccgcctcag gactcttcct ttttcaataa
     7201 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7261 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7321 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7381 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7441 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7501 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7561 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7621 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7681 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7741 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7801 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7861 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7921 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7981 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8041 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8101 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8161 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8221 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     8281 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     8341 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     8401 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     8461 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     8521 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     8581 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     8641 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttacg cgcgcgttg
//
