LOCUS       sequence-412249-        8591 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human HMGN3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8591)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 8591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-HMGN3
FEATURES             Location/Qualifiers
     source          1..8591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(8..28)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13..342
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      193..328
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     255..274
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        390..437
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             456..830
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    960..1081
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(997..1016)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1051..1070
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1130..1146)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1130..1146)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1143..1165)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1154..1170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1178..1184,1185..1202,1203..1208))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1223..1244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1361..1378)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1532..2120)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1612..1631)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2291..3082,3083..3151))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2914..2933
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3152..3256)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3331..3350)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3522..3901
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3903..4101
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             4119..4299
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4346..4471
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4964..5197
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5382..5426
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5724..5841
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    5898..5916
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5940..5958
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5982..6000
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6024..6042
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6066..6084
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6108..6126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        6159..6197
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6194..6218
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    6567..7155
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6620..6640)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7038..7049)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7158..7174)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7159..7175)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7680..7860
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7886..7903)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7892..8116
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8162..8590
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8249..8268)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8459..8480
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
       61 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
      121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
      181 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
      241 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
      301 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
      361 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
      421 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
      481 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
      541 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
      601 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
      661 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
      721 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
      781 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
      841 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
      901 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
      961 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     1021 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     1081 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     1141 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     1201 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1261 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     1321 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1381 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1441 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1501 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1561 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1621 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1681 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1741 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1801 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1861 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1921 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1981 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2041 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2101 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2161 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2221 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2281 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2341 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2401 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2461 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2521 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2581 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2641 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2701 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2761 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2821 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2881 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2941 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3001 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3061 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3121 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3181 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3241 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     3301 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3361 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     3421 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     3481 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     3541 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     3601 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     3661 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     3721 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     3781 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     3841 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     3901 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     3961 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     4021 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     4081 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
     4141 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     4201 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     4261 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     4321 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     4381 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     4441 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     4501 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     4561 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     4621 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     4681 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     4741 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     4801 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     4861 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     4921 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     4981 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     5041 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     5101 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     5161 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     5221 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     5281 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     5341 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     5401 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     5461 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     5521 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     5581 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     5641 acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga
     5701 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     5761 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     5821 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta
     5881 gatctcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     5941 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     6001 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     6061 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     6121 tagagaaaag tgaaagtcga gctcggtacc cgggtcgagg taggcgtgta cggtgggagg
     6181 cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
     6241 gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggcccc gaattcaccg
     6301 gttctagaat gccgaagaga aagtctccag agaatacaga gggcaaagat ggatccaaag
     6361 taactaaaca ggagcccaca agacggtctg ccagattgtc agcgaaacct gctccaccaa
     6421 aacctgaacc caaaccaaga aaaacatctg ctaagaaaga acctggagca aagattagca
     6481 gaggtgctaa agggaagaag gaggaaaagc aggaagctgg aaaggaaggc acagaaaact
     6541 gattcgaaga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     6601 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6661 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6721 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6781 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6841 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6901 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6961 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     7021 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7081 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7141 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca
     7201 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     7261 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     7321 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7381 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     7441 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     7501 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     7561 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     7621 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     7681 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7741 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7801 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7861 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     7921 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     7981 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     8041 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     8101 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     8161 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8221 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8281 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8341 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8401 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8461 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8521 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8581 aacgcgaatt a
//
