LOCUS       sequence-412252-        9944 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Mouse Runx2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9944)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 9944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-Runx2
FEATURES             Location/Qualifiers
     source          1..9944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2403..2421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2445..2463
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2487..2505
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2529..2547
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2571..2589
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2613..2631
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2664..2702
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2699..2723
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4425..5013
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4478..4498)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4896..4907)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5016..5032)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5017..5033)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5538..5718
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5744..5761)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5750..5974
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6020..6448
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6107..6126)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6317..6338
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6457..6477)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6462..6791
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6642..6777
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6704..6723
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6839..6886
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6905..7279
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7409..7530
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7446..7465)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7500..7519
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7579..7595)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7579..7595)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7592..7614)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7603..7619
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7627..7633,7634..7651,7652..7657))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7672..7693
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7810..7827)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7981..8569)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8061..8080)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8740..9531,9532..9600))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9363..9382
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9601..9705)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9780..9799)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
     2701 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     2761 gaagacaccg ggaccgatcc agcctccgcg gccccgaatt caccggttct agaatggcgt
     2821 caaacagcct cttcagcgca gtgacaccgt gtcagcaaag cttcttttgg gatccgagca
     2881 ccagccggcg cttcagcccc ccctccagca gcctgcagcc cggcaagatg agcgacgtga
     2941 gcccggtggt ggctgcgcag cagcagcaac agcagcagca gcagcagcaa cagcagcagc
     3001 aacaacagca acagcaacaa cagcagcagc agcagcagca gcaggaggcg gccgcagcag
     3061 cagcggcggc agcggcggcg gcagcagcgg cggcggccgc agtgccccga ttgaggccgc
     3121 cgcacgacaa ccgcaccatg gtggagatca tcgcggacca cccggccgaa ctggtccgca
     3181 ccgacagtcc caacttcctg tgctccgtgc tgccctcgca ctggcggtgc aacaagaccc
     3241 tgcccgtggc cttcaaggtt gtagccctcg gagaggtacc agatgggact gtggttaccg
     3301 tcatggccgg gaatgatgag aactactccg ccgagctccg aaatgcctcc gctgttatga
     3361 aaaaccaagt agccaggttc aacgatctga gatttgtggg ccggagcgga cgaggcaaga
     3421 gtttcacctt gaccataaca gtcttcacaa atcctcccca agtggccact taccacagag
     3481 ctattaaagt gacagtggac ggtccccggg aaccaagaag gcacagacag aagcttgatg
     3541 actctaaacc tagtttgttc tctgatcgcc tcagtgattt agggcgcatt cctcatccca
     3601 gtatgagagt aggtgtcccg cctcagaacc cacggccctc cctgaactct gcaccaagtc
     3661 cttttaatcc acaaggacag agtcagatta cagatcccag gcaggcacag tcttccccac
     3721 cgtggtccta tgaccagtct tacccctcct atctgagcca gatgacatcc ccatccatcc
     3781 actccaccac gccgctgtct tccacacggg gcaccgggct acctgccatc actgacgtgc
     3841 ccaggcgtat ttcagatgat gacactgcca cctctgactt ctgcctctgg ccttcctctc
     3901 tcagtaagaa gagccaggca ggtgcttcag aactgggccc tttttcagac cccaggcagt
     3961 tcccaagcat ttcatccctc actgagagcc gcttctccaa cccacgaatg cactacccag
     4021 ccacctttac ctacaccccg ccagtcacgt caggcatgtc cctcggcatg tccgccacca
     4081 ctcactacca cacgtacctg ccaccaccct accccggctc ttcccaaagc cagagtggac
     4141 ccttccagac cagcagcact ccatatctct actatggtac ttcgtcagca tcctatcagt
     4201 tcccaatggt acccggggga gaccggtctc cttccaggat ggtcccacca tgcaccacca
     4261 cctcgaatgg cagcacgcta ttaaatccaa atttgcctaa ccagaatgat ggtgttgacg
     4321 ctgacggaag ccacagcagt tccccaactg ttttgaattc tagcggcaga atggatgagt
     4381 ctgtttggcg gccatattga ttcgaagata tcaagcttat cgataatcaa cctctggatt
     4441 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     4501 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4561 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     4621 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     4681 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     4741 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     4801 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     4861 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4921 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4981 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag
     5041 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     5101 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     5161 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     5221 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     5281 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     5341 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     5401 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     5461 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     5521 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5581 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5641 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5701 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     5761 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     5821 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     5881 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     5941 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     6001 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6061 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6121 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6181 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6241 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6301 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6361 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6421 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     6481 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6541 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6601 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6661 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6721 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6781 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     6841 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     6901 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     6961 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     7021 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     7081 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     7141 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     7201 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     7261 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
     7321 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     7381 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     7441 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7501 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     7561 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7621 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7681 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7741 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7801 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7861 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7921 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7981 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8041 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8101 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8161 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8221 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8281 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8341 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8401 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8461 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8521 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8581 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8641 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8701 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8761 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8821 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8881 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8941 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9001 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9061 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9121 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9181 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9241 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9301 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9361 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9421 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9481 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9541 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9601 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9661 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9721 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     9781 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     9841 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     9901 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcg
//
