LOCUS       sequence-412270-        8759 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human ID3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 8759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-ID3
FEATURES             Location/Qualifiers
     source          1..8759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3240..3828
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3293..3313)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3711..3722)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3831..3847)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3832..3848)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4353..4533
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4559..4576)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4565..4789
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4835..5263
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4922..4941)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5132..5153
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5272..5292)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5277..5606
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5457..5592
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5519..5538
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5654..5701
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5720..6094
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6224..6345
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6261..6280)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6315..6334
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6394..6410)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6394..6410)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6407..6429)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6418..6434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6442..6448,6449..6466,6467..6472))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6487..6508
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6625..6642)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6796..7384)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6876..6895)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7555..8346,8347..8415))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8178..8197
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8416..8520)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8595..8614)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcaccggtt ctagaatgaa ggcgctgagc ccggtgcgcg
     2881 gctgctacga ggcggtgtgc tgcctgtcgg aacgcagtct ggccatcgcc cggggccgag
     2941 ggaagggccc ggcagctgag gagccgctga gcttgctgga cgacatgaac cactgctact
     3001 cccgcctgcg ggaactggta cccggagtcc cgagaggcac tcagcttagc caggtggaaa
     3061 tcctacagcg cgtcatcgac tacattctcg acctgcaggt agtcctggcc gagccagccc
     3121 ctggaccccc tgatggcccc caccttccca tccagacagc cgagctcgct ccggaacttg
     3181 tcatctccaa cgacaaaagg agcttttgcc actgattcga agatatcaag cttatcgata
     3241 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3301 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3361 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3421 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3481 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3541 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3601 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     3661 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3721 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3781 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     3841 gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     3901 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     3961 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     4021 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     4081 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga
     4141 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg
     4201 tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga
     4261 tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc
     4321 acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag
     4381 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     4441 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     4501 gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc
     4561 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     4621 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     4681 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     4741 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     4801 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     4861 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4921 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4981 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5041 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5101 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5161 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5221 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     5281 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5341 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     5401 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     5461 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5521 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     5581 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     5641 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     5701 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg
     5761 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt
     5821 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca
     5881 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct
     5941 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat
     6001 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa
     6061 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac
     6121 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     6181 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     6241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     6361 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6421 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6481 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6541 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6601 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6661 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6721 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6781 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6841 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6901 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6961 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7021 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7081 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7141 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7201 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7261 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7321 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7381 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7441 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7501 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7561 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7621 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7681 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7741 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7801 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7861 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7921 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7981 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8041 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8101 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8161 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8221 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8281 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8341 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8401 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8461 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8521 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     8581 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     8641 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     8701 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcg
//
