LOCUS       sequence-412274-       11627 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human NFATC3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11627)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 11627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-NFATC3
FEATURES             Location/Qualifiers
     source          1..11627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    6108..6696
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6161..6181)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6579..6590)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6699..6715)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6700..6716)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7221..7401
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7427..7444)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7433..7657
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7703..8131
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7790..7809)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8000..8021
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8140..8160)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8145..8474
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8325..8460
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8387..8406
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8522..8569
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8588..8962
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9092..9213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9129..9148)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9183..9202
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9262..9278)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9262..9278)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9275..9297)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9286..9302
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9310..9316,9317..9334,9335..9340))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9355..9376
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9493..9510)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9664..10252)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9744..9763)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10423..11214,11215..11283))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11046..11065
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11284..11388)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11463..11482)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcaccggtt ctagaatgac tactgcaaac tgtggcgccc
     2881 acgacgagct cgacttcaaa ctcgtctttg gcgaggacgg ggcgccggcg ccgccgcccc
     2941 cgggctcgcg gcctgcagat cttgagccag atgattgtgc atccatttac atctttaatg
     3001 tagatccacc tccatctact ttaaccacac cactttgctt accacatcat ggattaccgt
     3061 ctcactcttc tgttttgtca ccatcgtttc agctccaaag tcacaaaaac tatgaaggaa
     3121 cttgtgagat tcctgaatct aaatatagcc cattaggtgg tcccaaaccc tttgagtgcc
     3181 caagtattca aattacatct atctctccta actgtcatca agaattagat gcacatgaag
     3241 atgacctaca gataaatgac ccagaacggg aatttttgga aaggccttct agagatcatc
     3301 tctatcttcc tcttgagcca tcctaccggg agtcttctct tagtcctagt cctgccagca
     3361 gcatctcttc taggagttgg ttctctgatg catcttcttg tgaatcgctt tcacatattt
     3421 atgatgatgt ggactcagag ttgaatgaag ctgcagcccg atttaccctt ggatcccctc
     3481 tgacttctcc tggtggctct ccagggggct gccctggaga agaaacttgg catcaacagt
     3541 atggacttgg acactcatta tcacccaggc aatctccttg ccactctcct agatccagtg
     3601 tcactgatga gaattggctg agccccaggc cagcctcagg accctcatca aggcccacat
     3661 ccccctgtgg gaaacggagg cactccagtg ctgaagtttg ttatgctggg tccctttcac
     3721 cccatcactc acctgttcct tcacctggtc actcccccag gggaagtgtg acagaagata
     3781 cgtggctcaa tgcttctgtc catggtgggt caggccttgg ccctgcagtt tttccatttc
     3841 agtactgtgt agagactgac atccctctca aaacaaggaa aacttctgaa gatcaagctg
     3901 ccatactacc aggaaaatta gagctgtgtt cagatgacca agggagttta tcaccagccc
     3961 gggagacttc aatagatgat ggccttggat ctcagtatcc tttaaagaaa gattcatgtg
     4021 gtgatcagtt tctttcagtt ccttcaccct ttacctggag caaaccaaag cctggccaca
     4081 cccctatatt tcgcacatct tcattacctc cactagactg gcctttacca gctcattttg
     4141 gacaatgtga actgaaaata gaagtgcaac ctaaaactca tcatcgagcc cattatgaaa
     4201 ctgaaggtag ccgaggggca gtaaaagcat ctactggggg acatcctgtt gtgaagctcc
     4261 tgggctataa cgaaaagcca ataaatctac aaatgtttat tgggacagca gatgatcgat
     4321 atttacgacc tcatgcattt taccaggtgc atcgaatcac tgggaagaca gtcgctactg
     4381 caagccaaga gataataatt gccagtacaa aagttctgga aattccactt cttcctgaaa
     4441 ataatatgtc agccagtatt gattgtgcag gtattttgaa actccgcaat tcagatatag
     4501 aacttcgaaa aggagaaact gatattggca gaaagaatac tagagtacga cttgtgtttc
     4561 gtgtacacat cccacagccc agtggaaaag tcctttctct gcagatagcc tctatacccg
     4621 ttgagtgctc ccagcggtct gctcaagaac ttcctcatat tgagaagtac agtatcaaca
     4681 gttgttctgt aaatggaggt catgaaatgg ttgtgactgg atctaatttt cttccagaat
     4741 ccaaaatcat ttttcttgaa aaaggacaag atggacgacc tcagtgggag gtagaaggga
     4801 agataatcag ggaaaaatgt caaggggctc acattgtcct tgaagttcct ccatatcata
     4861 acccagcagt tacagctgca gtgcaggtgc acttttatct ttgcaatggc aagaggaaaa
     4921 aaagccagtc tcaacgtttt acttatacac cagttttgat gaagcaagaa cacagagaag
     4981 agattgattt gtcttcagtt ccatctttgc ctgtgcctca tcctgctcag acccagaggc
     5041 cttcctctga ttcagggtgt tcacatgaca gtgtactgtc aggacagaga agtttgattt
     5101 gctccatccc acaaacatat gcatccatgg tgacctcatc ccatctgcca cagttgcagt
     5161 gtagagatga gagtgttagt aaagaacagc atatgattcc ttctccaatt gtacaccagc
     5221 cttttcaagt cacaccaaca cctcctgtgg ggtcttccta tcagcctatg caaactaatg
     5281 ttgtgtacaa tggaccaact tgtcttccta ttaatgctgc ctctagtcaa gaatttgatt
     5341 cagttttgtt tcagcaggat gcaactcttt ctggtttagt gaatcttggc tgtcaaccac
     5401 tgtcatccat accatttcat tcttcaaatt caggctcaac aggacatctc ttagcccata
     5461 cacctcattc tgtgcatacc ctgcctcatc tgcaatcaat gggatatcat tgttcaaata
     5521 caggacaaag atctctttct tctccagtgg ctgaccagat tacaggtcag ccttcgtctc
     5581 agttacaacc tattacatat ggtccttcac attcagggtc tgctacaaca gcttccccag
     5641 cagcttctca tcccttggct agttcaccgc tttctgggcc accatctcct cagcttcagc
     5701 ctatgcctta ccaatctcct agctcaggaa ctgcctcatc accgtctcca gccaccagaa
     5761 tgcattctgg acagcactca actcaagcac aaagtacggg ccaggggggt ctttctgcac
     5821 cttcatcctt aatatgtcac agtttgtgtg atccagcgtc atttccacct gatggggcaa
     5881 ctgtgagcat taaacctgaa ccagaagatc gagagcctaa ctttgcaacc attggtctgc
     5941 aggacatcac tttagatgat gtgaacgaga taattgggag agacatgtcc cagatttctg
     6001 tttcccaagg agcaggggtg agcaggcagg ctcccctccc gagtcctgag tccctggatt
     6061 taggaagatc tgatgggctc taattcgaag atatcaagct tatcgataat caacctctgg
     6121 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     6181 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     6241 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     6301 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     6361 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6421 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6481 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     6541 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6601 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6661 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc
     6721 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     6781 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     6841 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     6901 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     6961 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     7021 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     7081 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     7141 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     7201 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7261 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7321 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7381 cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct
     7441 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     7501 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     7561 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     7621 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     7681 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     7741 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     7801 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     7861 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     7921 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     7981 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     8041 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     8101 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     8161 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     8221 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     8281 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     8341 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     8401 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     8461 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     8521 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     8581 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     8641 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     8701 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     8761 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     8821 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     8881 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     8941 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     9001 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     9061 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     9121 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9181 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     9241 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     9301 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     9361 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     9421 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     9481 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9541 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9601 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9661 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9721 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9781 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9841 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9901 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9961 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    10021 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    10081 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
    10141 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    10201 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    10261 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    10321 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    10381 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    10441 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    10501 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    10561 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10621 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10681 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10741 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10801 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10861 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10921 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10981 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    11041 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    11101 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    11161 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    11221 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    11281 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    11341 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    11401 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
    11461 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
    11521 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
    11581 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcg
//
