LOCUS       sequence-412344-        8615 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses the genetically-encoded fluorescent dopamine (DA) sensor 
            GRAB_gDA3m and a membrane-localized mcherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8615)
  AUTHORS   Zhuo Y, Luo B, Yi X, Dong H, Miao X, Wan J, Williams JT, Campbell 
            MG, Cai R, Qian T, Li F, Weber SJ, Wang L, Li B, Wei Y, Li G, Wang 
            H, Zheng Y, Zhao Y, Wolf ME, Zhu Y, Watabe-Uchida M, Li Y
  TITLE     Improved green and red GRAB sensors for monitoring dopaminergic 
            activity in vivo.
  JOURNAL   Nat Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02100-w.
  PUBMED    38036855
REFERENCE   2  (bases 1 to 8615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02100-w."
COMMENT     Sequence Label: pDisplay-GRAB-gDA3m-IRES-mCherry-CAAX
FEATURES             Location/Qualifiers
     source          1..8615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        29..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     657..676
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        657..675
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     756..818
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     protein_bind    819..843
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1742..1763
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1869..1890)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(2833..3406,3407..3409,3410..3418)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3000..3017)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3226..3245
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3419..4126
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3564..3582)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3832..3851
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4184..4213
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4217..4363
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4385..4402)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4391..4615
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4747..5335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4827..4846)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5664..5711
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5736..5755
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5943..6737)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTYGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6054..6073)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6664..6683
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(6836..6855)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        6934..7125
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     7082..7102
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(7185..7976,7977..8045))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7808..7827
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8046..8150)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8177..8605
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8264..8283)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8474..8495
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attaacgctt acaatttacg cgcgcgttga cattgattat tgactagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccctgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
      661 acgactcact atagggagac ccaagcttgg taccgagctc ggatccacta gtaacggccg
      721 ccagtgtgct ggaattcggc ttggggatat ccaccatgga gacagacaca ctcctgctat
      781 gggtactgct gctctgggtt ccaggttcca ctggtgacac aagtttgtac aaaaaagttg
      841 gcaccaccgg taggactctg aacacctctg ccatggacgg gactgggctg gtggtggaga
      901 gggacttctc tgttcgtatc ctcactgcct gtttcctgtc gctgctcatc ctgtccacgc
      961 tcctggggaa cacgctggtc tgtgctgccg ttatcaggtt ccgacacctg cggtccaagg
     1021 tgaccaactt ctttgtcatc tccttggctg tgtcagatct cttggtggcc gtcctggtca
     1081 tgccctggaa ggcagtggct gagattgctg gcttctggcc ctttgggtcc ttctgtaaca
     1141 tctgggtggc ctttgacatc atgtgctcca ctgcatccat cctcaacctc tgtgtgatca
     1201 gcgtggacag gtattgggct atctccagcc ctgcccggta tgagagaaag atgaccccca
     1261 aggcagcctt catcctgatc agtgtggcat ggaccttgtc tgtactcatc tccttcatcc
     1321 cagtgcagct cagctggcac aaggcaaaac ccacaagccc ctctgatgga aatgccactt
     1381 ccctggctga gaccatagac aactgtgact ccagcctcag caggacatat gccatctcat
     1441 cctctgtaat aagcttttac atccctgtgg ccatcatgat tgtcttgtac accaggatct
     1501 acaggattgc tcagaaacag ctgaattcac tcatttatgt ctatatcaag gccgacaagc
     1561 agaagaacgg catcaaggcg aacttcggga tccgccacaa catcgaggac ggtggcgtgc
     1621 agctcgccta ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1681 acaaccacta cctgagcgtg cagtccaaac tttcgaaaga ccccaacgag aagcgcgatc
     1741 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     1801 acaagggcgg taccggaggg ctgatggtga gcaagggcga ggagctgttc accggggtgg
     1861 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1921 agggtgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1981 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     2041 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     2101 acatccagga gcgcaccatc ttcttcaaga atgacggctt ttacaagacc cgcgccgagg
     2161 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2221 aggacggcaa catcctgggg cacaagctgg agtacaacaa tcatgaccaa ctgaaaagag
     2281 aaactaaagt cctgaagact ctgtcggtga tcatgggtgt gtttgtgtgc tgttggctac
     2341 ctttcttcat cttgaactgc attttgccct tctgtgggtc tggggagacg cagcccttct
     2401 gcattgattc caacaccttt gacgtgtttg tgtggtttgg gtgggctaat tcatccttga
     2461 accccatcat ttatgccttt aatgctgatt ttcggaaggc attttcaacc ctcttaggat
     2521 gctacagact ttgccctgcg acgaataatg ccatagagac ggtgagtatc aataacaatg
     2581 gggccgcgat gttttccagc catcatgagc cacgaggctc catctccaag gagtgcaatc
     2641 tggtttacct gatcccacat gctgtgggct cctctgagga cctgaaaaag gaggaggcag
     2701 ctggcatcgc cagacccttg gagaagctgt ccccagccct atcggtcata ttggactatg
     2761 acactgacgt ctctctggag aagatccaac ccatcacaca aaacggtcag cacccaacct
     2821 gataagctag cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
     2881 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     2941 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     3001 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     3061 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     3121 cgacaggtgc ctctgcggcc aacgccacgt gtataagata cacctgcaaa ggcggcacaa
     3181 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     3241 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     3301 gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     3361 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat
     3421 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     3481 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     3541 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     3601 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     3661 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     3721 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     3781 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     3841 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     3901 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     3961 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     4021 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     4081 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaaggatg gtaaaaagaa
     4141 gaaaaagaag tcaaagacaa agtgtgtaat tatgtaagtc gacgaacaaa aactcatctc
     4201 agaagaggat ctgaatgctg tgggccagga cacgcaggag gtcatcgtgg tgccacactc
     4261 cttgcccttt aaggtggtgg tgatctcagc catcctggcc ctggtggtgc tcaccatcat
     4321 ctcccttatc atcctcatca tgctttggca gaagaagcca cgttaggcgg ccgctcgaga
     4381 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     4441 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     4501 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     4561 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     4621 gaggcggaaa gaaccagtgg cggtaatacg gttatccaca gaatcggggg ataacgcagg
     4681 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4741 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4801 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4861 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4921 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4981 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5041 cggtaactat cgtcttgagt ccaacccggt aagacgcgac ttatcgccac tggcagcagc
     5101 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5161 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     5221 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5281 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5341 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5401 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     5461 ttttaaatca atctaaagta tatatgagta acctgaggct atggcagggc ctgccgcccc
     5521 gacgttggct gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga
     5581 agaaacgcgg gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc
     5641 ctgggaccga accccgcgtt tatgaacaaa cgacccaaca ccgtgcgttt tattctgtct
     5701 ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg tttcagttag
     5761 cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
     5821 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
     5881 gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc
     5941 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     6001 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     6061 cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg
     6121 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccataggtc
     6181 acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag ttcggctggc
     6241 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     6301 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     6361 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     6421 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     6481 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     6541 cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     6601 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     6661 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     6721 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat ctttgcaaaa
     6781 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgggcctcg
     6841 gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc
     6901 ggagttaggg gcggaatggg cggagttagg ggcgggacta tggttgctga ctaattgaga
     6961 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct
     7021 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
     7081 caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc ttcctttttc
     7141 aataaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7201 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     7261 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     7321 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7381 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7441 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7501 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7561 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7621 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7681 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7741 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7801 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7861 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7921 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7981 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8041 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8101 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     8161 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     8221 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     8281 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     8341 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     8401 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     8461 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     8521 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     8581 atttaacaaa aatttaacgc gaattttaac aaaat
//
