LOCUS       sequence-412767-         858 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian Expression Plasmid of SynDIG1/Tmem90b scFv. Derived from 
            hybridoma L42/17..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, 
            Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles 
            ML, Froenicke L, Trimmer JS
  TITLE     High-volume hybridoma sequencing on the NeuroMabSeq platform enables
            efficient generation of recombinant monoclonal antibodies and scFvs 
            for neuroscience research.
  JOURNAL   Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4.
  PUBMED    37758930
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-09-27"; volume: "13(1):16200. doi"; pages: " 
            10.1038/s41598-023-43233-4"
COMMENT     Sequence Label: SynDIG1/Tmem90b scFv [L42/17]
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             787..813
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             838..855
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggctgga gctgtattat tttgtttctt gtggctaccg ctactggagt ccattctcaa
       61 gtacaattgc aacaatcagg cgccgaactt atgaaaccag gcgcatccgt taaaatgagt
      121 tgtaaagcga cgggatatac ttttaacgat tattggatcg aatggatcaa acaaagaccc
      181 gggcacggac tcgaatggat aggggatatc ttcccgggca gcggcaggac aaattataac
      241 gaaaagttta aaggaaaagc tacttttaca gtggacacct cttctaatag cgcgtatatg
      301 cagcttagtt cactcaccag cgaagatagc gcggtgtact attgcagcaa cggcaactat
      361 tggggtcagg gaacaaccct taccgtgagt tccggaggag gtggatctgg cggtggtggt
      421 tcaggcggtg gcgggtccgg aggagggtcc gacgtgttaa tgactcagac ccctttaagc
      481 ttaccagtat cactcgggga ccaggcatct attagttgtc gtagttcaca aagtatcgtc
      541 cactccaacg gtaatacata cctcgagtgg tttcttcaaa agcccggaca atcacctaaa
      601 ctgcttattt ataaggtctc taataggttc tcaggtgtgc ccgatcggtt ttccggctcc
      661 ggcagtggca ctgactttac cttgaaaatt agtcgagtcg aagcggaaga cttaggtgtg
      721 tactattgtt tccagggatc ccacgtgccc tttacctttg gttcaggcac taaactggag
      781 attaagtatc catatgatgt acctgactat gcttccggcc tgccagaaac tggtgggcat
      841 catcatcatc atcactaa
//
