LOCUS       sequence-412914-        9421 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-IGSF3-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..9421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             6763..6807
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6808..6825
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6965..6982)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6971..7082
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7200..7220)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7205..7534
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7385..7520
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7447..7466
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7582..7629
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7648..8247
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7648..7667)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8144..8164
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8377..8498
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8414..8433)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8468..8487
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8551..9342,9343..9411))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9174..9193
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9412..9421,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccca aagacaagtg
     2761 acagtgcaag agggcccact gtacagaaca gagggcagcc acatcaccat ctggtgcaac
     2821 gtgtccggct atcagggccc cagcgagcag aatttccagt ggtccatcta cctgcctagc
     2881 agccccgaac gggaagtgca gatcgtgtct acaatggaca gcagcttccc ctacgccatc
     2941 tacacccaga gagtcagagg cggcaagatc ttcatcgaga gagtgcaggg caacagcacc
     3001 ctgctgcaca tcacagatct gcaggccaga gatgccggcg agtacgagtg tcacacccct
     3061 agcaccgaca agcagtactt cggcagctac agcgccaaga tgaacctggt ggtcatcccc
     3121 gatagcctgc agaccacagc catgcctcag acactgcaca gagtggaaca ggaccctctg
     3181 gaactgacat gcgaggtggc cagcgagaca atccagcact ctcatctgtc tgtggcctgg
     3241 ctgagacaga aagtgggcga gaagcccgtg gaagtgatca gcctgagccg ggactttatg
     3301 ctgcacagca gcagcgagta cgcccagaga cagagtctgg gagaagtgcg gctggacaag
     3361 ctgggcagaa ccaccttccg gctgaccatc ttccatctgc aacccagcga tcagggcgag
     3421 ttctactgtg aagccgccga gtggattcag gaccccgatg gatcttggta cgccatgacc
     3481 agaaagcgga gcgaaggcgc cgtggtcaat gtgcagccta ccgacaaaga attcaccgtg
     3541 cgcctggaaa ccgagaagag actgcacacc gtgggcgagc ctgtggaatt cagatgcatc
     3601 ctggaagccc agaacgtgcc cgacagatac tttgccgtgt cctgggcctt caacagcagc
     3661 ctgattgcca caatgggccc caatgctgtg cccgtgctga atagcgagtt tgcccacaga
     3721 gaggccagag gccagctgaa ggtggccaaa gaaagcgaca gcgtgttcgt gctgaagatc
     3781 taccacctga gacaagagga cagcggcaag tacaactgca gagtgaccga gcgggaaaag
     3841 accgtgaccg gcgagttcat cgacaaagag agcaagcggc ccaagaacat ccccatcatc
     3901 gtgctgcctc tgaagtccag catctctgtg gaagtggcca gcaacgccag cgtgatcctc
     3961 gaaggcgagg acctgagatt cagctgttct gtgcggacag ccggcagacc tcagggcaga
     4021 ttttctgtga tctggcagct ggtggaccgg cagaacagac ggtccaacat catgtggctg
     4081 gacagagatg gcaccgtgca gccaggcagc agctactggg agagaagctc ttttggcggc
     4141 gtccagatgg aacaagtgca gcccaatagc ttcagcctgg gcatcttcaa ctcccggaaa
     4201 gaggacgagg gccagtacga atgccacgtg acagagtggg tccgagccgt tgatggcgag
     4261 tggcagattg tgggagagag aagggccagc acacccatct ctatcacagc cctggaaatg
     4321 ggcttcgccg tgaccgccat tagcagaaca cctggcgtga cctacagcga cagcttcgac
     4381 ctgcagtgca tcatcaagcc tcactaccct gcctgggtgc cagtgtccgt gacttggaga
     4441 tttcagcctg tgggcaccgt ggaatttcac gacctggtca ccttcacaag agatggcgga
     4501 gtgcagtggg gcgacagaag cagcagcttt agaaccagaa ccgccatcga gaaggccgag
     4561 agcagcaaca atgtgcggct gagcatcagc agagccagcg atacagaggc cggaaagtac
     4621 cagtgtgtgg ccgagctgtg gcggaagaac tacaacaaca cctggaccag actggccgag
     4681 cggaccagca atctgctgga aatcagagtg ctgcagcccg tgaccaagct ccaggtgtcc
     4741 aagagcaaga gaaccctgac actggtggaa aacaagccca tccagctgaa ctgcagcgtg
     4801 aagtcccaga ccagccagaa cagccatttc gccgtgcttt ggtacgtgca caagccctct
     4861 gatgccgacg gcaagctgat cctgaaaacc acacacaaca gcgcctttga gtacggcacc
     4921 tacgccgagg aagaaggcct tagagccaga ctgcagttcg agcggcatgt gtctggcggc
     4981 ctgttttctc tgactgtgca gcgggccgaa gtgtccgata gcggcagcta ttactgccac
     5041 gtggaagaat ggctgctgag ccccaactac gcctggtata agctggccga agaggtgtcc
     5101 ggacggaccg aagtgactgt gaaacagccc gacagcagac tgaggctgtc tcaggctcag
     5161 ggcaatctga gcgtgctgga aacaagacag gtgcagctgg aatgcgtggt gctgaacaga
     5221 accagcatca ccagccagct gatggttgag tggttcgtgt ggaagcctaa tcaccccgag
     5281 agggaaaccg tggccagact gagtagagat gccaccttcc actatggcga gcaggccgct
     5341 aagaacaacc tgaagggcag actgcacctg gaatcaccct ctccaggcgt gtaccggctg
     5401 ttcatccaga atgtggccgt gcaggactct ggcacctaca gctgtcatgt cgaagagtgg
     5461 ctgccttctc cttccggcat gtggtacaag agagccgagg ataccgccgg acagacagcc
     5521 ctgacagtga tgaggcctga tgccagcctg caggttgaca ccgtggtgcc taatgccacc
     5581 gtgtctgaga aagccgcctt ccagctcgac tgctccatcg tgtccagaag ctcccaggac
     5641 agcagattcg ccgtggcctg gtacagcctg agaacaaaag ccggcggaaa gcggtctagc
     5701 cctggcctgg aagaacaaga ggaagagagg gaagaagaag aagaagagga cgacgacgac
     5761 gatgacgacc ccacagaaag aacagccctg ctgagcgtgg gacccgatgc tgtgtttgga
     5821 cctgagggct ctccttggga ggggagactg agattccaga gactgagccc cgtgctgtac
     5881 agactgacag tgctgcaagc ctctcctcag gataccggca actactcctg tcacgtcgag
     5941 gaatggctcc caagtcctca gaaagagtgg tacaggctga ccgaagaaga gagcgcccct
     6001 atcggcattc gggtgctcga tacaagcccc acactgcaga gcattatctg cagcaacgac
     6061 gctggcgcgc cgggcggcga caagacccac acctgccccc cctgcccagc tccagaactg
     6121 ctgggagggc cttccgtgtt cctgttcccc cccaagccca aggacaccct gatgatcagc
     6181 aggacccccg aggtgacctg cgtggtggtg gccgtgtccc acgaggaccc agaggtgaag
     6241 ttcaactggt acgtggacgg cgtggaggtg cacaacgcca agaccaagcc cagagaggag
     6301 cagtacaaca gcacctacag ggtggtgtcc gtgctgaccg tgctgcacca ggactggctg
     6361 aacggcaaag aatacaagtg caaagtctcc aacaaggccc tggctgcccc aatcgaaaag
     6421 acaatcagca aggccaaggg ccagccacgg gagccccagg tgtacaccct gccccccagc
     6481 cgggaggaga tgaccaagaa ccaggtgtcc ctgacctgtc tggtgaaggg cttctacccc
     6541 agcgatatcg ccgtggagtg ggagagcaac ggccagcccg agaacaacta caagaccacc
     6601 cccccagtgc tggacagcga cggcagcttc ttcctgtaca gcaagctgac cgtggacaag
     6661 tccaggtggc agcagggcaa cgtgttcagc tgcagcgtga tgcacgaggc cctgcacaac
     6721 cactacaccc agaagtccct gagcctgagc cccggcggca gcggtcttaa tgatattttc
     6781 gaggctcaga agattgagtg gcatgaacac caccaccatc accactaagg ccggcctgta
     6841 gtcactggca caacgcgtta cccggtaagc caatcgggta tacacggtcg tcatactgca
     6901 gacagggttc ttctactttg caagatagtc ttgagtagta aaataaatag atagagaaaa
     6961 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     7021 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     7081 tcacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     7141 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     7201 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7261 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     7321 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     7381 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7441 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     7501 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     7561 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     7621 taatacgaca aggtgaggaa ctaaaccatg accgagtaca agcccacggt gcgcctcgcc
     7681 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     7741 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     7801 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     7861 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     7921 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     7981 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     8041 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     8101 gagcgcgccg gggtgcccgc cttcctggag acatccgcgc cccgcaacct ccccttctac
     8161 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     8221 tgcatgaccc gcaagcccgg tgcctgacac gtgctacgag atttcgattc caccgccgcc
     8281 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     8341 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     8401 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8461 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     8521 tctagctaga gcttggcgta atcatggtca ttaccaatgc ttaatcagtg aggcacctat
     8581 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8641 tacgatacgg gagggcttac catctggccc cagcgctgcg atgataccgc gagaaccacg
     8701 ctcaccggct ccggatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8761 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8821 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc atcgctacag gcatcgtggt
     8881 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8941 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9001 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9061 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9121 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9181 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9241 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9301 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9361 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tattcttcct
     9421 t
//
