LOCUS       sequence-413004-       12194 bp    DNA     circular SYN 15-APR-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, 
            this enhances the expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12194)
  AUTHORS   Page KM, Heblich F, Davies A, Butcher AJ, Leroy J, Bertaso F, Pratt 
            WS, Dolphin AC
  TITLE     Dominant-negative calcium channel suppression by truncated 
            constructs involves a kinase implicated in the unfolded protein 
            response.
  JOURNAL   J Neurosci. 2004 Jun 9;24(23):5400-9. doi: 
            10.1523/JNEUROSCI.0553-04.2004.
  PUBMED    15190113
REFERENCE   2  (bases 1 to 12194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.0553-04"; journalName: "J Neurosci"; date: 
            "2004-06-9- 9"; volume: "24"; issue: "23"; pages: "5400-9"
COMMENT     Sequence Label: Cav2.2 pCAGGS
FEATURES             Location/Qualifiers
     source          1..12194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..660
                     /label=chicken beta-actin promoter
     intron          661..1677
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1601..1623
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1685..1704
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(9215..9234)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9280..9335
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9334..9353)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9696..9712)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9696..9712)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9709..9731)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9720..9736
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9744..9750,9751..9768,9769..9774))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9789..9810
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9869..10064
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9915..10050
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    10070..10204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10107..10126)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10161..10180
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10272..10289)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10443..11031)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10523..10542)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11202..11993,11994..12062))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11825..11844
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12063..12167)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggsgsg ccaggggggg gggggggggg ggaggggggg
      541 ggsggggsga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
      721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg kgggggtgcc gggcggggcg
     1261 gggccgcctc gggccggggr gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cctcgagggt
     1741 accgggcccc ccctcgaggt cgaagcttga tcatggtccg cttcggggac gagctgggcg
     1801 gccgctatgg gggcgcgggc ggcgcggagc gggcccgggg cggcggggcc ggcggggctg
     1861 gcggcccggg ccccgggggg ctgccgccgg gccagcgggt cctctacaaa cagtcgatcg
     1921 cgcagcgcgc gcggaccatg gcgctgtaca accccatccc ggtcaagcag aactgcttca
     1981 ccgtcaaccg ctcgctcttc gtcttcagcg aggacaacgt cgtccgcaaa tacgccaagc
     2041 gcatcaccga gtggcctccg ttcgagtaca tgatcctggc caccatcatc gccaactgca
     2101 tcgtgctggc cctggagcag cacctccctg acggggacaa gacgcctatg tccgagcgcc
     2161 tggatgacac cgagccctac ttcattggga tcttctgctt cgaggcgggg atcaagatcc
     2221 tggccctggg ctttgttctg cacaagggct cctatctgcg gaacggctgg aacgtcatgg
     2281 acttcgtggt agtcctcacg gggattcttg ccacagccgg aaccgacttc gacctgcgaa
     2341 cgctgagggc tgtgcgtgtg ctaaggcccc tgaagctggt gtctgggatt ccgagcttgc
     2401 aggtggtgct caagtccatc atgaaggcca tggtcccgct gctgcagatc gggctgctgc
     2461 tcttcttcgc catcctcatg tttgccatca tcggcctcga gttctacatg ggcaagttcc
     2521 acaaggcctg cttccccaac agcacagacc cagatcccgt gggtgacttc ccctgtggca
     2581 aggaggcccc ggcccggctc tgtgagggag acacggagtg ccgggagtac tgggccggac
     2641 ccaactttgg tatcaccaac tttgacaaca tcctgtttgc catcttgacc gtgttccagt
     2701 gcatcaccat ggagggctgg actgacatcc tctacaatac aaacgatgca gccggcaaca
     2761 cgtggaactg gctctacttc atccctctca tcatcatcgg ctccttcttc atgctcaacc
     2821 tggtgctggg tgtgctctca ggagagtttg ctaaggagcg cgagagagtg gagaaccgcc
     2881 gcgccttcct gaagctccgg cggcagcagc agattgagcg ggagctcaac gggtacctgg
     2941 agtggatctt caaggctgag gaagtcatgc tggctgagga ggacaggaac gcagaggaga
     3001 agtcccctct ggatgcagtg ttgaagagag cagctgccaa gaagagcaga agcgacctga
     3061 tccaggccga ggagggggag ggccggctca caggcctctg tgctcccggt tccccgttcg
     3121 cccgtgccag cctgaaaagc gggaagacgg agagctcgtc gtacttccgg aggaaggaga
     3181 agatgttccg cttcttcatc cggcgcatgg tgaaggcaca gagcttctac tggacggtgc
     3241 tgtgtgtggt ggcactgaac acactgtgcg tggccatggt gcactacaac cagccgcagc
     3301 ggctcaccac ggcattgtac tttgcagagt ttgttttcct gggtctcttc ctcacagaga
     3361 tgtccctgaa gatgtatggc ctggggccca ggagctactt ccggtcctcc ttcaactgtt
     3421 ttgactttgg ggtcatcgtg ggcagtatct ttgaagtggt ctgggctgct gtcaagccag
     3481 ggacctcctt cgggatcagt gttctgcggg ctctgcgctt actgagaatc ttcaaggtca
     3541 ccaaatactg gaactcccta cggaacctgg tggtgtctct gctcaactcc atgaagtcca
     3601 tcatcagcct cctcttcctg ctcttcctgt tcattgttgt ctttgctctg ctggggatgc
     3661 agctgtttgg aggccagttc aactttaaag acgagacccc aacgaccaac tttgacacct
     3721 tccctgctgc tatcctcacc gtcttccaga tcctgaccgg agaggactgg aatgcggtca
     3781 tgtaccacgg gatcgagtcg caaggtggcg tcagcagagg aatgttctcc tccttctact
     3841 tcattgtcct cactctgttt gggaactaca ccctgctgaa tgtcttcctg gccattgctg
     3901 tggacaacct tgccaatgct caggagctga ccaaggatga agaggagatg gaagaagcag
     3961 ccaatcagaa actcgccttg cagaaggcca aggaagtggc tgaagtcagc cccatgtctg
     4021 ctgcgaacat ctccattgcc gccaggcagc agaactcggc caaagcgcgc tcggtgtggg
     4081 agcagcgagc cagccagctg cggctgcaga acctgagggc cagctgcgag gcgctgtaca
     4141 gcgagatgga ccccgaggag aggctgcgct acgccaccgc gcgccacctg cggcccgacg
     4201 tgaagacgca cctggaccgg ccgctggtgg ttgagcccgg ccgcgacgct ccgcggggcc
     4261 ccccgggggg gaagtcccgg ccggatggct cggaggcccc cgagggtgcg gatcccccgc
     4321 gcaggcatca ccggcaccga gacaaggaca aggctcccgc cacggtcccc tcggcggggg
     4381 aacaggacag agccgaagct ctgagggcag agggcgggga gctggggccc cgcgaggagc
     4441 gggggcggcc acgccgcagc cgcagcaagg aggccccggg cgccccggag gtgcgcagcg
     4501 atcgcggccg tggcccgtgt cctgaaggtg gtcggcgcca ccaccggcgc gggtcaccgg
     4561 aggaggcagc tgagagggag ccccgccgcc accgcgcgca ccggcacggg cccgacccgg
     4621 gcaaggaggg cccagcgtcc gggaccaggg gcgagcggcg cgcacgacac cgcacgggcc
     4681 cccgggcgtg cccccgggag gctgagagca gcgaggagcc tgcgcggcgg caccgggctc
     4741 ggcacaaggc gccgcccacc caggagaccg cggagaagga caaggaggct gccgagaagg
     4801 ggggcgaggc cacggaggcc gagaaggaca aggaggctcg gaaccaccag cccaaggagc
     4861 tgccctgtga cctggaggcc atcggcatgc tgggagtggg cgctgtgcat acgctgccca
     4921 gcacctgtct gcagaaggtg gaggagcagc cagaggatgc ggacaaccag cggaacgtca
     4981 cccgcatggg tagtcagccc ccggacacca gcaccactgt gcacattcct gtgacgctga
     5041 ccggcccccc cggggagacc acggtagtgc ccagtggaaa cgtggacctg gaaagtcaag
     5101 cagaggggaa gaaggaggtg gaaaccagcg atgtgatgag gagcggccct cggcccattg
     5161 tcccgtacag ttccatgttc tgtttaagcc caaccaacct gctgcgccgc tgctgccact
     5221 acattgtgac catgcgatac ttcgagatgg tcatcctcgt ggtcatcgcc ctgagcagca
     5281 tcgccctggc tgccgaggac cccgtgagaa cagactctcc caggaataat gctctgaagt
     5341 acatggacta catcttcaca ggcgtcttca ccttcgagat ggtgataaag atgatcgatt
     5401 tgggactgct gctacaccct ggagcctatt tccgggatct gtggaacatt ctggacttca
     5461 ttgtggtcag tggggccctg gtggcatttg ctttctcagg atccaaaggg aaagacatca
     5521 gcaccatcaa atccctgaga gtcctgcgtg tgctgcggcc actcaagacc atcaagcggc
     5581 tgcccaagct caaggccgtg tttgactgtg tggtgaactc actgaaaaac gtcctcaaca
     5641 tcctgatcgt ctacatgctc ttcatgttca tctttgccgt cattgccgtg cagctcttca
     5701 aagggaagtt cttctactgc acggacgaat ccaaggagct ggagcgtgac tgcaggggtc
     5761 agtatttgga ttatgagaag gaggaagtgg aagctcaacc gaggcagtgg aagaaatatg
     5821 acttccacta tgacaacgtg ctctgggctc tgctaacgct cttcacggtg tccacagggg
     5881 aagggtggcc catggtgctg aaacactctg tggatgccac ctacgaggag cagggcccga
     5941 gccccgggta ccgcatggag ctctccatct tctacgtggt ctactttgtg gtctttccct
     6001 tcttctttgt caacatcttc gtggccttga tcatcatcac cttccaggag cagggggaca
     6061 aggtgatgtc cgagtgcagc ctggagaaga acgagagggc ttgcatcgac tttgccatca
     6121 gcgccagacc cctgacgcgg tacatgcccc agaacaagca gtcgttccag tataagacgt
     6181 ggacgttcgt ggtgtcccca ccttttgagt acttcatcat ggctatgatc gccctcaaca
     6241 ctgtggtgct gatgatgaag ttctacgatg cgccgtacga gtatgagcta atgctgaaat
     6301 gcctgaacat cgtgttcacg tccatgttct ccatggagtg tgtgctgaag atcatcgcct
     6361 tcggggtgct gaactatttc agagatgcct ggaatgtctt tgactttgtc acggtgttgg
     6421 gaagtattac tgatattttg gtaacagaga ttgcgaacaa cttcatcaac ctcagcttcc
     6481 tgcgcctgtt ccgggccgcg cggctcatca agctgctgcg ccagggctac accatccgca
     6541 tcctgctttg gaccttcgtg cagtccttca aggcgctgcc ctacgtgtgt ctcctcatcg
     6601 ccatgctctt cttcatctac gccatcattg gcatgcaggt ttttgggaac atcgccctgg
     6661 acgatgacac cagcatcaac aggcacaata acttccggac gtttctgcag gccttgatgt
     6721 tgctgttcag gagcgccacg ggggaggcct ggcacgagat aatgctgtcg tgcctcagca
     6781 gccgggcctg cgacgagcac tccaatgcca gcgagtgcgg cagcgacttt gcctactttt
     6841 acttcgtctc cttcatcttc ctctgctcct ttctgatgtt gaacctcttt gtggctgtga
     6901 tcatggacaa ttttgagtac cttacacggg actcttccat cttagggcct caccacctgg
     6961 atgagttcat ccgggtctgg gctgaatacg acccggccgc gtgtgggcgc atcagttaca
     7021 gtgacatgtt tgagatgctg aagcacatgt ccccacccct ggggctgggg aagaagtgcc
     7081 ctgctcgagt cgcgtacaag cgcctggtcc gcatgaacat gcccatctcc agtgaggaca
     7141 tgacggtgca cttcacatcc acgctgatgg ctctcattcg gacagcgctg gacatcaagc
     7201 tggcaccagc tgggaccaag cagcaccagt gcgacgcaga gctgaggaag gagatttcct
     7261 gtgtgtgggc caatctgccc cagaagaccc tggacttact ggtaccaccc cataagcctg
     7321 acgagatgac ggtgggcaaa gtctatgcag ctctgatgat cttcgacttc tacaaacaga
     7381 acaaaaccag cagagaccag actcagcaag cccccggagg cctgtcccag ctgggccccg
     7441 tgtccctgtt ccacccgctg aaggccacgc tggagcagac tcagccggcg ctgcgtggag
     7501 cccgggcttt cctgcggcag aagagttctg cctccctgag caacggcggg gccgtacaaa
     7561 cccaggagag cggcatcaag gaatcggttt cttggggcac tcagaggacc caggatgtgc
     7621 tttgcgaggc cagggcaccc ctggagcggg gccactcagc tgagatccca gtcgggcagc
     7681 ctgggacact ggctgtagat gtccagatgc agaacatgac tctgagtggc cctgacgccg
     7741 agccgcagcc agggctggag agccagggcc gagccgcctc catgccccgc ctggccgcag
     7801 aaacacagcc cgcgcctgac gccagcccca tgaagcgctc catctccacg ctggccccgc
     7861 gcccccacac ggcccggctc ggcagcaccg cgctggaccg cccagccccc agccaggccc
     7921 cgcaccatca ccaccaccgc tgccaccgcc gcagggacag gaagcagcgg tccctggaga
     7981 agggtcccag cctgtctgct gacacagatg gggcccccga cagcacggtg gggccaggac
     8041 tgcccacggg ggagggcccc cccggctgca ggcgggagcg ggagcgcagg caggagcggg
     8101 gccggtccca ggagcggagg cagccctcgt cctcctcctc ggagaagcat cgcttctact
     8161 cctgtgaccg cttcgggggc cgcgaacccc cacagcccaa gccctccctc agtagtcacc
     8221 ccacgtcgcc gacggccggg caggagccgg gaccccaccc gcaggggagt ggttcggttc
     8281 acgggagccc cctgctgtcc acatctggtg ctagcacccc gggccggggg cggaggcagc
     8341 tcccccagac ccccttgacc ccccgcccca gcgtcaccta caagacggcc aactcctcgc
     8401 ctgtccactt cgccggggca ccgagcggcc tccccgcctt ctcccccggc cggctcagcc
     8461 ggggcctctc agaacacaac gccctgctgc agagggaccc gctcagccgg cccctggcgc
     8521 ccggctcgcg catcggctcc gacccctacc tggggcagcg gctggacagc gaggcgcccg
     8581 cccgtgccct gcccgaggac gcgcccgcct ttgaggagac cgcggccagc aactcgggcc
     8641 gctcctcccg gacctcgtac gtgtcctcac tgacctcgca gcccccgccg ctgcgccgcg
     8701 tgcccaacgg ctaccactgc actctggggc tgggcggggg cggccgggcg cggcgcggct
     8761 gccaccaccc cgaccgcgac cgccggtgct agccgctgcg cgcccgcccc cggcggtggg
     8821 cttcatcttc cgcgctgggc tgcggggacg cgggcaccga ggccctggac ggggcctggg
     8881 cggaggcccc tgctcccctc cccctcctcc ctgacagcag acggatcaat aaagcccctt
     8941 ccggcgggac gcggctgtct ctgccccctg acgtgctgcc ttcccaggtc tcccaccaca
     9001 ggctgatcct gtgtcagaga ccccggtgtc agtgtgcagg ggtggggggt gcggggcctg
     9061 tgtcggaccc ccagggttaa tggggccgga attcgcatgc ggccgctagc tcgaggtacc
     9121 gatatcgagc tcaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg
     9181 tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg
     9241 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
     9301 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     9361 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc
     9421 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg
     9481 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
     9541 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
     9601 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat
     9661 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     9721 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     9781 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     9841 tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc
     9901 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     9961 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    10021 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat
    10081 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    10141 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    10201 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    10261 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    10321 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    10381 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    10441 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    10501 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    10561 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    10621 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    10681 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    10741 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    10801 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    10861 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    10921 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    10981 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    11041 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    11101 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    11161 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    11221 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    11281 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    11341 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    11401 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    11461 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    11521 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    11581 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    11641 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    11701 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    11761 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    11821 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    11881 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    11941 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    12001 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    12061 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    12121 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    12181 aaagtgccac ctgg
//
