LOCUS       sequence-413373-        9662 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian expression of ATP11C isoform b mutant E184Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Inoue H, Takatsu H, Hamamoto A, Takayama M, Nakabuchi R, Muranaka Y,
            Yagi T, Nakayama K, Shin HW
  TITLE     The interaction of ATP11C-b with ezrin contributes to its polarized 
            localization.
  JOURNAL   J Cell Sci. 2021 Oct 15;134(20):jcs258523. doi: 10.1242/jcs.258523. 
            Epub 2021 Oct 18.
  PUBMED    34528675
REFERENCE   2  (bases 1 to 9662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-15"; volume: "134(20):jcs258523. doi"; pages: 
            " 10.1242/jcs.258523. Epub 2021 Oct 18"
COMMENT     Sequence Label: pMx/ATP11C-b(E184Q)-HA
FEATURES             Location/Qualifiers
     source          1..9662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1938..1962
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5073..5084)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     protein_bind    complement(5368..5392)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5424..5450
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(5489..5509)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5494..5823
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5674..5809
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6180..6974
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6234..6253)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6844..6863
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             7167..7636
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7766..7783)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7937..8525)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8017..8036)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8689..9480,9481..9549))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9312..9331
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9550..9654)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 ccctagttaa gctatcaaca agtttgtaca aaaaagcagg ctctttaaag gaaccaattc
     1981 agtcgactgg atccaccatg cagatggtcc catctctccc tccagcctct gagtgtgctg
     2041 gagaagagaa acgagttggc acacgcacag tgtttgttgg caatcatcca gtttcggaaa
     2101 cagaagctta cattgcacaa agattttgtg ataatagaat agtctcatct aagtatacac
     2161 tttggaattt tctcccaaag aatctgtttg aacagtttag aagaattgca aatttttatt
     2221 ttctcataat cttccttgta caggtcacag tagacacacc aactagccca gttaccagtg
     2281 gacttccact tttctttgtt ataactgtta cagccatcaa gcagggatat gaggattggc
     2341 tgagacacag agctgacaat gaagtcaaca aaagcactgt ttacattatt gaaaatgcaa
     2401 agcgagtgag aaaagaaagt gaaaaaatca aggttggtga tgtagtagaa gtacaggcag
     2461 atgaaacctt tccctgtgat cttattcttc tatcatcttg caccactgat ggaacctgtt
     2521 atgtcactac agccagtctt gatgggcaat ccaattgcaa gacacattat gcagtacgtg
     2581 ataccattgc actgtgtaca gcagaatcca tcgataccct ccgagcagca attgaatgtg
     2641 aacagcctca acctgacctc tacaaatttg ttgggcgaat caatatctac agtaatagtc
     2701 ttgaggctgt tgccaggtct ttgggacctg aaaatctctt gctgaaagga gctacgctaa
     2761 aaaataccga gaagatatat ggagttgctg tttacactgg aatggaaacc aaaatggctt
     2821 tgaactacca agggaaatct cagaaacgtt ctgctgttga aaaatctatt aatgctttcc
     2881 tgattgtata tttatttatc ttactgacca aagctgcagt atgcactact ctaaagtatg
     2941 tttggcaaag taccccatac aatgatgaac cttggtataa ccaaaagact cagaaagagc
     3001 gagagacctt gaaggtttta aaaatgttca ccgacttcct atcatttatg gttctattca
     3061 actttatcat tcctgtctcc atgtacgtca cagtagaaat gcagaaattc ttgggctcct
     3121 tcttcatctc atgggataag gacttttatg atgaagaaat taatgaagga gccctggtta
     3181 acacatcaga ccttaatgaa gaacttggtc aggtggatta tgtatttaca gataagactg
     3241 gaacactcac tgaaaacagc atggaattca ttgaatgctg catagatggc cacaaatata
     3301 aaggtgtaac tcaagaggtt gatggattat ctcaaactga tggaacttta acatattttg
     3361 acaaagtaga taagaatcga gaagagctgt ttctacgtgc cttgtgttta tgtcatactg
     3421 tagaaatcaa aacaaacgat gctgttgatg gagctacaga atcagctgaa ttaacctata
     3481 tctcctcttc accagatgaa atagctttgg tgaaaggagc taaaaggtac gggttcacat
     3541 ttttaggaaa tcgaaatgga tatatgagag tagagaacca aagaaaagaa atagaagaat
     3601 atgaacttct tcacacctta aactttgatg ctgtccggcg acgtatgagt gtaattgtga
     3661 agactcaaga aggagacata cttctctttt gtaaaggagc agactcggca gtttttccca
     3721 gagtgcaaaa tcatgaaatt gagttaacta aagtccatgt ggaacgtaat gcaatggatg
     3781 ggtatcggac actctgtgta gccttcaaag aaattgctcc agatgattat gaaagaatta
     3841 acagacagct catagaggca aaaatggcct tacaagacag agaagaaaaa atggaaaaag
     3901 ttttcgatga tattgagaca aacatgaatt taattggagc cactgcagtt gaagacaagc
     3961 tacaagatca agctgcagag accattgaag ctctgcatgc agcaggcctg aaagtctggg
     4021 tgctcactgg ggacaagatg gagacagcta aatccacatg ctatgcctgc cgccttttcc
     4081 agaccaacac tgagctctta gaactaacca caaaaaccat tgaagaaagt gaaaggaaag
     4141 aagatcgatt acatgaatta ttgatagaat atcgcaagaa attgctgcat gagtttccta
     4201 aaagtactag aagctttaaa aaagcatgga cagaacatca ggaatatgga ttaatcatag
     4261 atggctccac attgtcactc atactaaatt ctagtcaaga ctctagttca aacaattaca
     4321 aaagcatttt cctacaaata tgtatgaagt gtactgcagt gctctgctgt cggatggcac
     4381 cattacagaa agcccagatt gtcagaatgg tgaagaattt aaaaggcagc ccaataactc
     4441 tgtcgatagg tgatggtgcc aatgatgtta gtatgatctt ggaatcccat gtgggaatag
     4501 gtattaaagg caaagaaggt cgccaagcag ctaggaatag cgattattct gttccaaagt
     4561 ttaaacactt aaagaaactg ctgttggctc atggacatct atattatgtg agaatagcac
     4621 accttgtaca gtacttcttc tataagaacc tttgtttcat tttgccacag tttttgtacc
     4681 agttcttctg tggattctca caacagccac tgtatgatgc tgcttacctt acaatgtaca
     4741 atatctgctt cacatccttg cccatcctgg cctatagtct actggaacag cacatcaaca
     4801 ttgacactct gacctcagat ccccgattgt atatgaaaat ttctggcaat gccatgctac
     4861 agttgggccc cttcttatat tggacatttc tggctgcctt tgaagggaca gtgttcttct
     4921 ttgggactta ctttcttttt cagactgcat ccctagaaga aaatggaaag gtatacggaa
     4981 actggacttt tggaaccatt gtttttacag tcttagtatt cactgtaacc ctgaagcttg
     5041 ccttggatac ccgattctgg acgtggataa atcactttgt gatttggggt tctttagcct
     5101 tctatgtatt tttctcattc ttctggggag gaattatttg gccttttctc aagcaacaga
     5161 gaatgtattt tgtatttgcc caaatgctgt cttctgtatc cacatggttg gctataattc
     5221 ttctaatatt tatcagcctg ttccctgaga ttcttctgat agtattaaag aatgtaagaa
     5281 gaagaagtgc caggaatccg aatcttgaac tgcctatgtt attgtcctac aagcatactg
     5341 acagtggtta cagctcgaga tatctagacc cagctttctt gtacaaagtg gttgatctag
     5401 agggcccgcg gttcgaactc gagtatccat acgatgtacc agactacgca gagatccact
     5461 agcggccgcc agcacagtgg tcgactgtgg aatgtgtgtc agttagggtg tggaaagtcc
     5521 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5581 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5641 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5701 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5761 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5821 aaaaagcttc acgctgccgc aagcactcag ggcgcaaggg ctgctaaagg aagcggaaca
     5881 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
     5941 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     6001 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     6061 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     6121 ggatctgatg gcgcagggga tcaagatctg atcaagagac aggatgagga tcgtttcgca
     6181 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     6241 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     6301 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     6361 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     6421 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     6481 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     6541 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     6601 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     6661 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     6721 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     6781 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     6841 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     6901 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     6961 acgagttctt ctgagcggga ctctggggtt cgcatcgtcg acgataaaat aaaagatttt
     7021 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc
     7081 ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag
     7141 atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag
     7201 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag
     7261 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat
     7321 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga
     7381 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     7441 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag
     7501 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
     7561 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
     7621 cagcgggggt ctttcacatg cagcatgtat caaaattaat ttggtttttt ttcttaagta
     7681 tttacattaa atggccatag ttgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7741 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7801 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7861 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7921 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7981 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8041 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8101 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8161 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8221 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8281 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8341 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8401 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8461 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8521 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8581 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttgcggc
     8641 cggccgcaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8701 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8761 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8821 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8881 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8941 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9001 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9061 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9121 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9181 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9241 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9301 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9361 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9421 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9481 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9541 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9601 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9661 tc
//
