LOCUS       sequence-413614-       13177 bp    DNA     circular SYN 14-APR-2025
DEFINITION  doxycycline-inducible expression of TAOK3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13177)
  AUTHORS   Ma X, Mandausch FJ, Wu Y, Sahoo VK, Ma W, Leoni G, Hostiuc M, 
            Wintgens JP, Qiu J, Kannaiyan N, Rossner MJ, Wehr MC
  TITLE     Comprehensive split TEV based protein-protein interaction screening 
            reveals TAOK2 as a key modulator of Hippo signalling to limit 
            growth.
  JOURNAL   Cell Signal. 2024 Jan;113:110917. doi: 
            10.1016/j.cellsig.2023.110917. Epub 2023 Oct 7.
  PUBMED    37813295
REFERENCE   2  (bases 1 to 13177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Signal."; date: "2024-01"; volume: "113:110917. doi"; pages: " 
            10.1016/j.cellsig.2023.110917. Epub 2023 Oct 7"
COMMENT     Sequence Label: pINDUCER21-puro_TAOK3
FEATURES             Location/Qualifiers
     source          1..13177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5426..5450)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5503..5568
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5586..5612
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5832..7010
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6063..7001
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6958..6978
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    7080..7113
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(7120..7737,7738..7743,7744..7779,7780..7782,7783..7818,
                     7819..7821,7822..7857,7858..7866)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    join(7919..8493,8494..8496,8497..8505)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8086..8103)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8313..8332
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8525..9124
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8525..8544)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9021..9041
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9260..9279
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    9567..10155
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9620..9640)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10038..10049)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10230..10463
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10582..10600)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10668..10772
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10773..10841,10842..11633)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10991..11010)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11804..12392
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12293..12312
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12546..12563
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12803..12824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12839..12844,12845..12862,12863..12869)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12877..12893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12882..12904
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12901..12917
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12901..12917
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12967..12986
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctccacc atgcgtaaag gggtgctgaa ggacccagag
     2761 attgccgatc tattctacaa agatgatcct gaggaacttt ttattggttt gcatgaaatt
     2821 ggacatggaa gttttggagc agtttatttt gctacaaatg ctcacaccag tgaggtggtg
     2881 gcaattaaga agatgtccta tagtgggaag cagacccatg agaaatggca agatattctt
     2941 aaggaagtta aatttttacg acaattgaag catcctaata ctattgagta caaaggctgt
     3001 tacttgaaag aacacactgc ttggttggtg atggaatatt gcttaggctc agcctctgat
     3061 ttattagaag ttcataaaaa accacttcag gaagtggaga tcgctgccat tactcatgga
     3121 gccttgcatg gactagccta cctacattct catgcattga ttcataggga tattaaagca
     3181 ggaaatattc ttctaacaga gccaggtcag gtaaaactag ctgattttgg atctgcttca
     3241 atggcttctc ctgccaactc cttcgtgggc acaccttact ggatggctcc agaggtgatc
     3301 ttagctatgg atgaaggaca gtatgatggg aaagttgata tttggtcact tggcatcact
     3361 tgtattgaat tggcggaacg gaagccgccc cttttcaaca tgaatgcaat gagtgcctta
     3421 tatcacattg cccagaatga ctccccaacg ttacagtcta atgaatggac agactccttt
     3481 aggagatttg ttgattactg cttgcagaaa atacctcagg aaaggccaac atcagcagaa
     3541 ctattaaggc atgactttgt tcgacgagac cggccactac gtgtcctcat tgacctcata
     3601 cagaggacaa aagatgcagt tcgtgagcta gataacctac agtaccgaaa aatgaaaaaa
     3661 atacttttcc aagagacacg gaatggaccc ttgaatgagt cacaggagga tgaggaagac
     3721 agtgaacatg gaaccagcct gaacagggaa atggacagcc tgggcagcaa ccattccatt
     3781 ccaagcatgt ccgtgagcac aggcagccag agcagcagtg tgaacagcat gcaggaagtc
     3841 atggacgaga gcagttccga acttgtcatg atgcacgatg acgaaagcac aatcaattcc
     3901 agctcctccg tcgtgcataa gaaagatcat gtattcataa gggatgaggc gggccacggc
     3961 gatcccaggc ctgagccgcg gcctacccag tcagttcaga gccaggccct ccactaccgg
     4021 aacagagagc gctttgccac gatcaaatca gcatctttgg ttacacgaca gatccatgag
     4081 catgagcagg agaacgagtt gcgggaacag atgtcaggtt ataagcggat gcggcgccag
     4141 caccagaagc agctgatcgc cctggagaac aagctgaagg ctgagatgga cgagcaccgc
     4201 ctcaagctac agaaggaggt ggagacgcat gccaacaact cgtccatcga gctggagaag
     4261 ctggccaaga agcaagtggc tatcatagaa aaggaggcaa aggtagctgc agcagatgag
     4321 aagaagttcc agcaacagat cttggcccag cagaagaaag atttgacaac tttcttagaa
     4381 agtcagaaga agcagtataa gatttgtaag gaaaaaataa aagaggaaat gaatgaggac
     4441 catagcacac ccaagaaaga gaagcaagag cggatctcca aacataaaga gaacttgcag
     4501 cacacacagg ctgaagagga agcccacctt ctcactcaac agagactgta ctacgacaaa
     4561 aattgtcgtt tcttcaagcg gaaaataatg atcaagcggc acgaggtgga gcagcagaac
     4621 attcgggagg aactaaataa aaagaggacc cagaaggaga tggagcatgc catgctaatc
     4681 cggcacgacg agtccacccg agagctagag tacaggcagc tgcacacgtt acagaagcta
     4741 cgcatggatc tgatccgttt acagcaccag acggaactgg aaaaccagct ggagtacaat
     4801 aagaggcgag aaagagaact gcacagaaag catgtcatgg aacttcggca acagccaaaa
     4861 aacttaaagg ccatggaaat gcaaattaaa aaacagtttc aggacacttg caaagtacag
     4921 accaaacagt ataaagcact caagaatcac cagttggaag ttactccaaa gaatgagcac
     4981 aaaacaatct taaagacact gaaagatgag cagacaagaa aacttgccat tttggcagag
     5041 cagtatgaac agagtataaa tgaaatgatg gcctctcaag cgttacggct agatgaggct
     5101 caagaagcag aatgccaggc cttgaggcta cagctccagc aggaaatgga gctgctcaac
     5161 gcctaccaga gcaaaatcaa gatgcaaaca gaggcacaac atgaacgtga gctccagaag
     5221 ctagagcaga gagtgtctct gcgcagagca caccttgagc agaagattga agaggagctg
     5281 gctgcccttc agaaggaacg cagcgagaga ataaagaacc tattggaaag gcaagagcga
     5341 gagattgaaa cttttgacat ggagagcctc agaatgggat ttgggaattt ggttacatta
     5401 gattttccta aggaggacta cagagaccca gctttcttgt acaaagtggt tgatatccag
     5461 cacagtggcg gccgctcgag tctagagggc ccgcggttcg aagactacaa agaccatgac
     5521 ggtgattata aagatcatga catcgattac aaggatgacg atgacaagta ggtaagtgat
     5581 tcgaataccc atacgacgtc ccagactacg cttagtaatg attaattaaa ctagaaattc
     5641 taccgggtag gggaggcgct tttcccaagg cagtctggag taacccaccc aagatctggc
     5701 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agccgcgtaa
     5761 gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag
     5821 cctagtcgtg aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     5881 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
     5941 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     6001 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     6061 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
     6121 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg
     6181 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
     6241 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
     6301 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
     6361 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
     6421 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
     6481 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
     6541 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
     6601 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
     6661 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
     6721 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
     6781 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
     6841 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
     6901 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
     6961 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga agcggccgcc
     7021 atgctagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacggatcca
     7081 taacttcgta taatgtatgc tatacgaagt tatgccacca tgtctagact ggacaagagc
     7141 aaagtcataa acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca
     7201 aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac
     7261 aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc
     7321 tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc
     7381 tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag
     7441 aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg
     7501 gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag
     7561 gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc
     7621 ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt
     7681 ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg
     7741 ccgaccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac
     7801 tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc
     7861 gggtaactaa gtaaggatcc gcggccgcac tagaggaatt ccgcccctct cccccccccc
     7921 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     7981 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     8041 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     8101 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     8161 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     8221 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     8281 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     8341 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     8401 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     8461 acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccacaa ggagacgacc
     8521 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     8581 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     8641 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     8701 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     8761 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     8821 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     8881 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     8941 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     9001 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     9061 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     9121 ctgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     9181 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     9241 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     9301 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     9361 ttctatcgcc ttcttgacga gttcttctga tgtacaagta aagcggccgc gactctagat
     9421 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     9481 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gtttagtccc tcccaattcg
     9541 atatcaagct tatcgcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat
     9601 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     9661 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     9721 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     9781 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     9841 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     9901 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     9961 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
    10021 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
    10081 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
    10141 gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct
    10201 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
    10261 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
    10321 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    10381 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    10441 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
    10501 acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag
    10561 atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
    10621 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    10681 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    10741 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    10801 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    10861 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    10921 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    10981 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
    11041 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    11101 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    11161 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    11221 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    11281 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    11341 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    11401 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    11461 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    11521 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    11581 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    11641 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    11701 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    11761 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    11821 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    11881 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    11941 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    12001 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    12061 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    12121 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    12181 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    12241 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    12301 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    12361 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    12421 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    12481 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    12541 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    12601 ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt tttttattta
    12661 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    12721 tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag
    12781 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    12841 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    12901 caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt
    12961 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    13021 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    13081 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    13141 attgtatttg ttaaatatgt actacaaact tagtagt
//
