LOCUS       sequence-413694-        8815 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentivirus for inducible knockdown of human AHR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  TITLE     Halawani et al. bHLH-PAS in neuroregeneration
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLKO-Tet-On-AHR-shRNA2
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    400..525
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             572..752
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(753..979)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1007..1027)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1007..1025)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1046..1062)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1046..1062)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1059..1081)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1070..1086
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1094..1100,1101..1118,1119..1124))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1139..1160
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1277..1294)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1448..2036)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1528..1547)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2207..2998,2999..3067))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2830..2849
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3068..3172)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3198..3653)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3335..3356)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3547..3566
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3780..3802
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3794..3811
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3795..3811
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3821..3839
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3860..3995
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4035..4156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4046..4065)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4100..4119
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4228..4461
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(4589..5188)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4672..4692)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5169..5188
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    5208..5781
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5368..5387)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     5597..5614
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(5816..6436)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     6344..6363
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        complement(6447..6465)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6493..6512)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          6520..7092
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        complement(7101..7611)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7164..7182)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    7660..7777
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(7877..7971)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    7877..7895
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7905..7923
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(7941..7960)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(8260..8304)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8489..8722
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc
       61 cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc
      121 cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata
      181 taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg
      241 tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt ctagctccct
      301 gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat
      361 tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct
      421 ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc
      481 tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc
      541 tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag
      601 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
      661 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
      721 caggctcaga tctggtctaa ccagagagac ccgtttatgt atcgagctag gcacttaaat
      781 acaatatctc tgcaatgcgg caattcagtg gttcgtccaa tccatgtcag acccgtctgt
      841 tgccttccta ataaggcacg atcgtaccac cttacttcca ccaatcggca tgcacggtgc
      901 tttttctctc cttgtaaggc atgttgctaa ctcatcgtta ccatgttgca agactacaag
      961 agtattgcat aagactacat taagcttgca gctccagctt ttgttccctt tagtgagggt
     1021 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1081 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     1141 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     1201 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     1261 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1321 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1381 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1441 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1501 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1561 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1621 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1681 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1741 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1801 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1861 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1921 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1981 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2041 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2101 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2161 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2221 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2281 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2341 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2401 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2461 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2521 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2581 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2641 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2701 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2761 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2821 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2881 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     2941 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3001 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3061 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3121 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3181 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     3241 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     3301 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     3361 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     3421 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     3481 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     3541 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     3601 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     3661 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     3721 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     3781 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     3841 cgaattgggt acgtccctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     3901 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     3961 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggatagct
     4021 agagccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     4081 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     4141 ctgcaataaa caagttcctc tcactctctg atattcattt ctttgcaagt tataaatact
     4201 gaataataag atgacatgaa ctactactgc tagagatttt ccacactgac taaaagggtc
     4261 tgagggatct ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc
     4321 aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg
     4381 ctcagatctg gtctaaccag agagacccag tacaagcaaa aagcagatct tgtcttcgtt
     4441 gggagtgaat tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag
     4501 ctgccttgta agtcattggt cttaaaggta ccgatgcatg gggtcgtgcg ctcctttcgg
     4561 tcgggcgctg cgggtcgtgg ggcgggcgtc aggcaccggg cttgcgggtc atgcaccagg
     4621 tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc cgctcgtaga
     4681 aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg cgctcggccg
     4741 cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg tcgggcgaga
     4801 cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc aggaggcctt
     4861 ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc gggccgatct
     4921 cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc accgcggcgc
     4981 cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg aagagttctt
     5041 gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc gtggcggggt
     5101 agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg cgggtggcga
     5161 ggcgcaccgt gggcttgtac tcggtcatgg taagctccgg tggatcctta tcatcgtgtt
     5221 tttcaaagga aaaccacgtc cccgtggttc ggggggccta gacgtttttt taacctcgac
     5281 taaacacatg taaagcatgt gcaccgaggc cccagatcag atcccataca atggggtacc
     5341 ttctgggcat ccttcagccc cttgttgaat acgcttgagg agagccattt gactctttcc
     5401 acaactatcc aactcacaac gtggcactgg ggttgtgccg cctttgcagg tgtatcttat
     5461 acacgtggct tttggccgca gaggcacctg tcgccaggtg gggggttccg ctgcctgcaa
     5521 agggtcgcta cagacgttgt ttgtcttcaa gaagcttcca gaggaactgc ttccttcacg
     5581 acattcaaca gaccttgcat tcctttggcg agaggggaaa gacccctagg aatgctcgtc
     5641 aagaagacag ggccaggttt ccgggccctc acattgccaa aagacggcaa tatggtggaa
     5701 aataacatat agacaaacgc acaccggcct tattccaagc ggcttcggcc agtaacgtta
     5761 gggggggggg agggagaggg gcagatccct agaactcccg ggagccgctg tacgcggacc
     5821 cactttcaca tttaagttgt ttttctaatc cgcatatgat caattcaagg ccgaataaga
     5881 aggctggctc tgcaccttgg tgatcaaata attcgatagc ttgtcgtaat aatggcggca
     5941 tactatcagt agtaggtgtt tccctttctt ctttagcgac ttgatgctct tgatcttcca
     6001 atacgcaacc taaagtaaaa tgccccacag cgctgagtgc atataatgca ttctctagtg
     6061 aaaaaccttg ttggcataaa aaggctaatt gattttcgag agtttcatac tgtttttctg
     6121 taggccgtgt acctaaatgt acttttgctc catcgcgatg acttagtaaa gcacatctaa
     6181 aacttttagc gttattacgt aaaaaatctt gccagctttc cccttctaaa gggcaaaagt
     6241 gagtatggtg cctatctaac atctcaatgg ctaaggcgtc gagcaaagcc cgcttatttt
     6301 ttacatgcca atacaatgta ggctgctcta cacctagctt ctgggcgagt ttacgggttg
     6361 ttaaaccttc gattccgacc tcattaagca gctctaatgc gctgttaatc actttacttt
     6421 tatctaatct agacatatca attcgcccta tagtgagtcg tattacaatt ctttgccaaa
     6481 atgatgagac agcacaataa ccagcacgtt gcccaggagc tgtaggaaaa agaagaaggc
     6541 atgaacatgg ttagcagagg ggcccggttt ggactcagag tattttatcc tcatctcaaa
     6601 cagtgtatat cattgtaacc ataaagagaa aggcaggatg atgaccagga tgtagttgtt
     6661 tctaccaata agaatatttc cacgccagcc agaatttata tgcagaaata ttctacctta
     6721 tcatttaatt ataacaattg ttctctaaaa ctgtgctgaa gtacaatata atataccctg
     6781 attgccttga aaaaaaagtg attagagaaa gtacttacaa tctgacaaat aaacaaaagt
     6841 gaatttaaaa attcgttaca aatgcaagct aaagtttaac gaaaaagtta cagaaaatga
     6901 aaagaaaata agaggagaca atggttgtca acagagtaga aagtgaaaga aacaaaatta
     6961 tcatgagggt ccatggtgat acaagggaca tcttcccatt ctaaacaaca ccctgaaaac
     7021 tttgccccct ccatataaca tgaattttac aatagcgaaa aagaaagaac aatcaagggt
     7081 ccccaaactc acagatcccc ctggggagag aggtcggtga ttcggtcaac gagggagccg
     7141 actgccgacg tgcgctccgg aggcttgcag aatgcggaac accgcgcggg caggaacagg
     7201 gcccacacta ccgccccaca ccccgcctcc cgcaccgccc cttcccggcc gctgctctcg
     7261 gcgcgccctg ctgagcagcc gctattggcc acagcccatc gcggtcggcg cgctgccatt
     7321 gctccctggc gctgtccgtc tgcgagggta ctagtgagac gtgcggcttc cgtttgtcac
     7381 gtccggcacg ccgcgaaccg caaggaacct tcccgactta ggggcggagc aggaagcgtc
     7441 gccggggggc ccacaagggt agcggcgaag atccgggtga cgctgcgaac ggacgtgaag
     7501 aatgtgcgag acccagggtc ggcgccgctg cgtttcccgg aaccacgccc agagcagccg
     7561 cgtccctgcg caaacccagg gctgccttgg aaaaggcgca accccaaccc cctcgagccg
     7621 cggccaaagt ggatctctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat
     7681 ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc
     7741 tgcactgtac cccccaatcc ccccttttct tttaaaattg tggatgaata ctgccatttg
     7801 tctcgaggtc gagaattaaa aaatccacag tcagccataa taactcgagt tattatggct
     7861 gactgtggat ccggtgtctc tatcactgat agggaactta taagtctcta tcactgatag
     7921 ggatttcacg tttatggtga tttcccagaa cacatagcga catgcaaata ttgcggccgc
     7981 tcgaggctag tctcgtgatc gataccgtcg agatccgttc actaatcgaa tggatctgtc
     8041 tctgtctctc tctccacctt cttcttctat tccttcgggc ctgtcgggtc ccctcggggt
     8101 tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga
     8161 aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta tgaataattt
     8221 tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatctaa
     8281 ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa ttaaggagtg
     8341 tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg tgtgattcca
     8401 aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag tggtgcaaat
     8461 gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt atctttccac
     8521 agccaggatt cttgcctgga gctgcttgat gccccagact gtgagttgca acagatgctg
     8581 ttgcgcctca atagccctca gcaaattgtt ctgctgctgc actataccag acaataattg
     8641 tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa
     8701 gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca ccactcttct
     8761 ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat attta
//
